Brain hsp70 expression and DNA damage in mice exposed to extremely low frequency magnetic fields: A dose-response study

Villarini, Milena; Ambrosini, Maria Vittoria; Moretti, Massimo; Dominici, Luca; Taha, Elena; Piobbico, Danilo; Gambelunghe, Cristiana; Mariucci, Giuseppina

doi:10.3109/09553002.2013.782449

Cited by 10 publications

(4 citation statements)

References 63 publications

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“…The expression on hsp70 mRNA in the brain tissues of CD1 mice exposed to 50 magnetic fields was investigated by Villarini et al (2013). Animals were exposed to 0.1, 0.2, 1 or 2 mT for 15h/day for 7 days using a pair of rectangular coils in a Helmholtz-like configuration.…”

Section: Mechanistic Studiesmentioning

confidence: 99%

Ecological study on the penetration of induction heating cookers and birth outcomes in Japan

et al. 2020

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About the Scientific CommitteesThree independent non-food Scientific Committees provide the Commission with the scientific advice it needs when preparing policy and proposals relating to consumer safety, public health and the environment. The Committees also draw the Commission's attention to the new or emerging problems which may pose an actual or potential threat.They are: the Scientific Committee on Consumer Safety (SCCS), the Scientific Committee on Health and Environmental Risks (SCHER) and the Scientific Committee on Emerging and Newly Identified Health Risks (SCENIHR).In addition, the Commission relies upon the work of the European Food Safety Authority (EFSA), the European Medicines Agency (EMA), the European Centre for Disease prevention and Control (ECDC) and the European Chemicals Agency (ECHA). SCENIHRThis Committee deals with questions related to emerging or newly identified health and environmental risks and on broad, complex or multidisciplinary issues requiring a comprehensive assessment of risks to consumer safety or public health and related issues not covered by other Community risk assessment bodies. Examples of potential areas of activity include potential risks associated with interaction of risk factors, synergic effects, cumulative effects, antimicrobial resistance, new technologies such as nanotechnologies, medical devices including those incorporating substances of animal and/or human origin, tissue engineering, blood products, fertility reduction, cancer of endocrine organs, physical hazards such as noise and electromagnetic fields (from mobile phones, transmitters and electronically controlled home environments), and methodologies for assessing new risks. It may also be invited to address risks related to public health determinants and non-transmissible diseases.

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Section: Mechanistic Studiesmentioning

confidence: 99%

Ecological study on the penetration of induction heating cookers and birth outcomes in Japan

et al. 2020

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“…According to recent literature, ELF‐MF exposure is believed to play a role in the later stages (promotion and progression) of tumorigenesis, especially in brain‐derived tumors (Poulletier de Gannes et al, ; Turner et al, ). Critical cellular pathways and functions have been proposed as targets of ELF magnetic fields: DNA damage profile (Panagopoulos et al, ; Villarini et al, ; Yoon et al, ), proliferation and/or survival dynamics (Martínez et al, ; Trillo et al, ; Razavi et al, ), signaling pathways (Nie and Henderson, ; Patruno et al, ), and redox balance (Falone et al, ; Falone et al, ; Di Loreto et al, ; Mattsson and Simkó, ). We have previously shown that the exposure to an ELF magnetic field increases the proliferation of cells isolated from neuroblastoma, and this was linked to the novel expression of protein associated to a more malignant phenotype (Sulpizio et al, ).…”

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confidence: 99%

Improved Mitochondrial and Methylglyoxal‐Related Metabolisms Support Hyperproliferation Induced by 50 Hz Magnetic Field in Neuroblastoma Cells

Falone

Santini

Loreto

et al. 2016

Journal Cellular Physiology

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Extremely low frequency magnetic fields (ELF-MF) are common environmental agents that are suspected to promote later stages of tumorigenesis, especially in brain-derived malignancies. Even though ELF magnetic fields have been previously linked to increased proliferation in neuroblastoma cells, no previous work has studied whether ELF-MF exposure may change key biomolecular features, such as anti-glycative defence and energy re-programming, both of which are currently considered as crucial factors involved in the phenotype and progression of many malignancies. Our study investigated whether the hyperproliferation that is induced in SH-SY5Y human neuroblastoma cells by a 50 Hz, 1 mT ELF magnetic field is supported by an improved defense towards methylglyoxal (MG), which is an endogenous cancer-static and glycating α-oxoaldehyde, and by rewiring of energy metabolism. Our findings show that not only the ELF magnetic field interfered with the biology of neuron-derived malignant cells, by de-differentiating further the cellular phenotype and by increasing the proliferative activity, but also triggered cytoprotective mechanisms through the enhancement of the defense against MG, along with a more efficient management of metabolic energy, presumably to support the rapid cell outgrowth. Intriguingly, we also revealed that the MF-induced bioeffects took place after an initial imbalance of the cellular homeostasis, which most likely created a transient unstable milieu. The biochemical pathways and molecular targets revealed in this research could be exploited for future approaches aimed at limiting or suppressing the deleterious effects of ELF magnetic fields. J. Cell. Physiol. 231: 2014-2025, 2016. © 2016 Wiley Periodicals, Inc.

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“…Whether ELF-MFs are able to induce hsp70 expression still remains a controversial issue. For example, hsp70 has been found to increase after ELF-MF exposure (Tokalov and Gutzeit, 2004; George et al, 2008; Blank and Goodman, 2009), while others reported negative results (Mariucci et al, 2010; Villarini et al, 2017, 2013). Because the experimental conditions were different from each other in regard to equipment used to generate MFs, exposure protocol (frequency, flux intensity and/or time) and biological material (cell lines and animal species, strain and age), the results of various studies may be different.…”

Section: Discussionmentioning

confidence: 98%

Exposure to 50 Hz magnetic field at 100 µT exert no DNA damage in cardiomyocytes

Wang

Liu²,

Zhang³

et al. 2019

Biology Open

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The effects of exposure to magnetic fields (MFs) at electric frequencies (50–60 Hz) on carcinogenicity are still in debate. Whether exposure to MFs affects the heart is also a debated issue. This study aimed to determine whether exposure to extremely low frequency MFs (ELF-MFs) induced DNA damage in cardiomyocytes both in vitro and in vivo. Human ventricular cardiomyocytes were exposed to 50 Hz ELF-MF at 100 µT for 1 h continuously or 75 min intermittently. The effects of the treatments were evaluated by DNA damage, redox status changes and relative signal molecular expression. Moreover, ten male Sprague-Dawley rats were exposed to a 50 Hz MF at 100 µT for 7 days, while another 10 rats were sham exposed. The protein levels of p53 and Hsp70 in heart tissue were analyzed by western blot. The results showed that exposure to ELF-MF did not induce DNA damage, changes to cell cycle distribution or increased reactive oxygen species level. No significant differences were detected in p53 and Hsp70 expression level between the ELF-MF and sham-exposure groups both in vitro and in vivo. All these data indicate that MFs at power-frequency may not cause DNA damage in cardiomyocytes.

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Brain hsp70 expression and DNA damage in mice exposed to extremely low frequency magnetic fields: A dose-response study

Abstract: These data show that high density ELF-MF only induce reversible brain DNA damage while they do not affect hsp70 expression.

Cited by 10 publications

References 63 publications

Ecological study on the penetration of induction heating cookers and birth outcomes in Japan

Ecological study on the penetration of induction heating cookers and birth outcomes in Japan

Improved Mitochondrial and Methylglyoxal‐Related Metabolisms Support Hyperproliferation Induced by 50 Hz Magnetic Field in Neuroblastoma Cells

Exposure to 50 Hz magnetic field at 100 µT exert no DNA damage in cardiomyocytes

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