Brain histopathology in red tilapia <i>Oreochromis</i>
 sp. experimentally infected with <i>Streptococcus agalactiae</i>
 serotype III

Palang, Iyapa; Withyachumnarnkul, Boonsirm; Senapin, Saengchan; Sirimanapong, Wanna; Vanichviriyakit, Rapeepun

doi:10.1002/jemt.23481

Cited by 9 publications

(9 citation statements)

References 56 publications

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“…Therefore, it was possible that the strong densities of TiLV in these areas were linked with erratic swimming or loss of balance. An earlier study also suggested that Streptococcus agalactiae infection caused damage in the cerebellum of tilapia and led to the erratic swimming signs (Palang et al., 2020). Taken together, localization of TiLV with high density detected in important regions of the brain may contribute to an array of well‐described abnormal signs, especially neurological disorder signs during the course of infection, and result in high mortality.…”

Section: Discussionmentioning

confidence: 97%

Dissecting the localization of Tilapiatilapinevirus in the brain of the experimentally infected Nile tilapia, Oreochromisniloticus (L.)

Dinh-Hung

Sangpo

Kruangkum

et al. 2021

Journal of Fish Diseases

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Tilapia tilapinevirus or tilapia lake virus (TiLV) is an emerging virus that inflicts significant mortality on farmed tilapia globally. Previous studies reported detection of the virus in multiple organs of the infected fish; however, little is known about the in‐depth localization of the virus in the central nervous system. Herein, we determined the distribution of TiLV in the entire brain of experimentally infected Nile tilapia. In situ hybridization (ISH) using TiLV‐specific probes revealed that the virus was broadly distributed throughout the brain. The strongest positive signals were dominantly detected in the forebrain (responsible for learning, appetitive behaviour and attention) and the hindbrain (involved in controlling locomotion and basal physiology). The permissive cell zones for viral infection were observed mostly to be along the blood vessels and the ventricles. This indicates that the virus may productively enter into the brain through the circulatory system and widen broad regions, possibly through the cerebrospinal fluid along the ventricles, and subsequently induce the brain dysfunction. Understanding the pattern of viral localization in the brain may help elucidate the neurological disorders of the diseased fish. This study revealed the distribution of TiLV in the whole infected brain, providing new insights into fish–virus interactions and neuropathogenesis.

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Section: Discussionmentioning

confidence: 97%

Dissecting the localization of Tilapiatilapinevirus in the brain of the experimentally infected Nile tilapia, Oreochromisniloticus (L.)

Dinh-Hung

Sangpo

Kruangkum

et al. 2021

Journal of Fish Diseases

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show abstract

“…Therefore, it was possible that the strong densities of TiLV in these areas were linked with with erratic swimming or loss of balance. An earlier study also suggested that Streptococcus agalactiae infection caused damage in the cerebellum of tilapia and led to the erratic swimming symptoms (Palang, Withyachumnarnkul, Senapin, Sirimanapong, & Vanichviriyakit, 2020). Taken together, localization of TiLV with high density detected in important regions of the brain may contribute to an array of well-described abnormal symptoms, especially neurological disorder signs during the course of infection, and result in high mortality.…”

Section: Gc Tm 823 O C)mentioning

confidence: 92%

Dissecting the localization ofTilapia tilapinevirusin the brain of the experimentally infected Nile tilapia (Oreochromis niloticus)

Dinh-Hung

Sangpo

Kruangkum

et al. 2021

Preprint

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Tilapia tilapinevirus or tilapia lake virus (TiLV) is an emerging virus that inflicts significant mortality on farmed tilapia globally. Previous studies reported detection of the virus in multiple organs of the infected fish; however, little is known about the in-depth localization of the virus in the central nervous system. Herein, we determined the distribution of TiLV in the entire brain of experimentally infected Nile tilapia. In situ hybridization (ISH) using TiLV-specific probes revealed that the virus was broadly distributed throughout the brain. The strongest positive signals were dominantly detected in the forebrain (responsible for learning, appetitive behavior, and attention) and the hindbrain (involved in controlling locomotion and basal physiology). The permissive cell zones for viral infection were observed mostly to be along the blood vessels and the ventricles. This indicates that the virus may productively enter into the brain through the circulatory system and widen broad regions, possibly through the cerebrospinal fluid along the ventricles, and subsequently induce the brain dysfunction. Understanding the pattern of viral localization in the brain may help elucidate the neurological disorders of the diseased fish. This study revealed the distribution of TiLV in the whole infected brain, providing new insights into fish-virus interactions and neuropathogenesis.

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“…Streptococcus agalactiae serotype III in this study was originally isolated from a field outbreak, kept and maintained in the laboratory before the experiment (Palang et al., 2020). A single colony of the bacteria was inoculated in 20 ml Tryptic Soy Broth (TSB) for 24 hr and expanded into 1 L, in four bottles, under different temperature levels: 22, 29, 32 and 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…In farmed Nile ( Oreochromis niloticus ) and red ( Oreochromis sp.) tilapia, the infection sites are the brain, liver, kidney, spleen and skin (Filho, Müller, & Pretto‐Giordano, 2009; Hernández, Figueroa, & Iregui, 2009; Palang, Withyachumnarnkul, Senapin, Sirimanapong, & Vanichviriyakit, 2020; Suanyuk, Kong, Ko, Gilbert, & Supamattaya, 2008). Several lines of evidence suggest that the bacteria, commonly termed Group B Streptococcus (GBS), are persistently residing in water environment (Amal & Zamri‐Saad, 2011; Nguyen, Kanai, & Yoshikoshi, 2002), in fish body and even in terrestrial animals, including in human, and their existence in hosts does not necessarily lead to streptococcosis unless the bacteria are induced to secrete certain virulence factors (capsular, surface and extracellular proteins) that damage host tissues (Yen‐Hsi Liu & Nizet, 2004).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Streptococcus agalactiae secretory protein on tilapia cultured cells

Palang

Hirono

Senapin

et al. 2020

Journal of Fish Diseases

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Streptococcus agalactiae and S. iniae are bacterial pathogens infecting several types of farmed fish, especially in tilapia and sea bass, leading to erratic swimming, exophthalmos, skin lesion and other signs known as streptococcosis (Bromage & Owens, 2002; Mian et al., 2009; Zamri-Saad, Amal, & Siti-Zahrah, 2010). In farmed Nile (Oreochromis niloticus) and red (Oreochromis sp.) tilapia, the infection sites are the brain, liver, kidney, spleen and skin (Filho, Müller, &

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Brain histopathology in red tilapia Oreochromis sp. experimentally infected with Streptococcus agalactiae serotype III

Cited by 9 publications

References 56 publications

Dissecting the localization of Tilapiatilapinevirus in the brain of the experimentally infected Nile tilapia, Oreochromisniloticus (L.)

Dissecting the localization of Tilapiatilapinevirus in the brain of the experimentally infected Nile tilapia, Oreochromisniloticus (L.)

Dissecting the localization ofTilapia tilapinevirusin the brain of the experimentally infected Nile tilapia (Oreochromis niloticus)

Cytotoxicity of Streptococcus agalactiae secretory protein on tilapia cultured cells

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