Cytotoxicity of <i>Streptococcus agalactiae</i> secretory protein on tilapia cultured cells

Palang, Iyapa; Hirono, Ikuo; Senapin, Saengchan; Sirimanapong, Wanna; Withyachumnarnkul, Boonsirm; Vanichviriyakit, Rapeepun

doi:10.1111/jfd.13230

Cited by 6 publications

(11 citation statements)

References 40 publications

(57 reference statements)

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“…Bacterial pellets were suspended in enzymatic lysis buffer (20 mM Tris-Cl pH 8; 2 mM Sodium EDTA; 1.2% Trition ® X-100; 100 mg/mL lysozyme) and incubated at 37 • C overnight, and the remainder of the extraction was conducted as per the manufacturer's instructions. Species identity of Thai isolates was confirmed by PCR, using forward primer gyrA_F (5 -GCACAATGGTGGTCATATCG-3 ) and reverse primer gyrA-R (5 -ACGCGCTGGTAAAACAAGAG-3 ), which target the DNA gyrase subunit A region [32]. The PCR mixture contained 2.5 µL of 10× PCR buffer, 0.5 µL of 10 mM dNTP, 0.5 µL of each forward and reverse primers, 1 µL of template DNA, 0.25 µL of Taq polymerase, and ddH 2 O to a final volume of 20 µL.…”

Section: Molecular Identificationmentioning

confidence: 99%

Geographical, Temporal and Host-Species Distribution of Potentially Human-Pathogenic Group B Streptococcus in Aquaculture Species in Southeast Asia

et al. 2023

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Group B Streptococcus (GBS) is a major pathogen of humans and aquatic species. Fish have recently been recognized as the source of severe invasive foodborne GBS disease, caused by sequence type (ST) 283, in otherwise healthy adults in Southeast Asia. Thailand and Vietnam are among the major aquaculture producers in Southeast Asia, with GBS disease reported in fish as well as frogs in both countries. Still, the distribution of potentially human-pathogenic GBS in aquaculture species is poorly known. Using 35 GBS isolates from aquatic species in Thailand collected from 2007 to 2019 and 43 isolates from tilapia collected in Vietnam in 2018 and 2019, we have demonstrated that the temporal, geographical, and host-species distribution of GBS ST283 is broader than previously known, whereas the distribution of ST7 and the poikilothermic lineage of GBS are geographically restricted. The gene encoding the human GBS virulence factor C5a peptidase, scpB, was detected in aquatic ST283 from Thailand but not in ST283 from Vietnam or in ST7 from either country, mirroring current reports of GBS strains associated with human sepsis. The observed distribution of strains and virulence genes is likely to reflect a combination of spill-over, host adaptation through the gain and loss of mobile genetic elements, and current biosecurity practices. The plastic nature of the GBS genome and its importance as a human, aquatic, and potentially foodborne pathogen suggests that active surveillance of GBS presence and its evolution in aquaculture systems may be justified.

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Section: Molecular Identificationmentioning

confidence: 99%

Geographical, Temporal and Host-Species Distribution of Potentially Human-Pathogenic Group B Streptococcus in Aquaculture Species in Southeast Asia

et al. 2023

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“…Tilapia kidney (TK-1) cell line is another interesting cell line for virus isolation and propagation. This cell line has previously been used to determine the cytotoxicity of secretory products from Streptococcus agalactiae ( 49 , 50 ). However, the viral susceptibility of TK-1, especially to TiLV infection remains unexplored and could be used as a diagnostic tool for TiLV infection.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, the CPEs of TiLV infected TK-1 were clearly visible compared with mock TK-1, with heavy intracellular vacuolation and detachment observed at the late stage of TiLV infection. The CPEs of TiLV infected TK-1 differed from TiLV infected OnlL, which demonstrated cell shrinkage and aggregation, plaque formation and syncytial formation ( 49 ), while granularity and elongation of cells followed by rounding and destruction of the monolayer were observed in OnlB cells ( 48 ). CPEs in TiLV infected TK-1 were similar to those observed in TiLV infected E-11, TG and TH cells, with the appearance of swollen, rounded and cytoplasmic vacuoles clearly observed from day 5 post-inoculation, and the cells become vastly detached thereafter ( 28 , 47 ).…”

Section: Discussionmentioning

confidence: 99%

Combining segments 9 and 10 in DNA and recombinant protein vaccines conferred superior protection against tilapia lake virus in hybrid red tilapia (oreochromis sp.) compared to single segment vaccines

et al. 2022

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Tilapia lake virus (TiLV) now affects Nile tilapia culture worldwide, with no available commercial vaccine for disease prevention. DNA and recombinant protein-based vaccines were developed and tested following viral isolation and characterization. The viral strain isolated from diseased hybrid red tilapia (Oreochromis sp.) shared high levels of morphological and genomic similarity (95.49-99.52%) with other TiLV isolates in the GenBank database. TiLV segment 9 (Tis9) and segment 10 (Tis10) DNA vaccines (pcDNA-Tis9 and pcDNA-Tis10) and recombinant protein vaccines (Tis9 and Tis10) were prepared and tested for their efficacy in juvenile hybrid red tilapia. Fish were immunized with either single vaccines (pcDNA-Tis9, pcDNA-Tis10, Tis9 and Tis10) or combined vaccines (pcDNA-Tis9 + pcDNA-Tis10 and Tis9 + Tis10) by intramuscular injection and intraperitoneal injection for DNA and protein vaccines, respectively. Negative controls were injected with PBS or a naked pcDNA3.1 vector in the same manner. An experimental challenge with TiLV was carried out at 4 weeks post-vaccination (wpv) by intraperitoneal injection with a dose of 1 × 105 TCID50 per fish. Relative percent survival (RPS) ranged from 16.67 ± 00.00 to 61.11 ± 9.62%. The Tis10 and pcDNA-Tis10 vaccines conferred better protection compared to Tis9 and pcDNA-Tis9. Highest levels of protection were observed in pcDNA-Tis9 + pcDNA-Tis10 (61.11 ± 9.62%) and Tis9 + Tis10 (55.56 ± 9.62%) groups. Specific antibody was detected in all vaccinated groups at 1-4 wpv by Dot Blot method, with the highest integrated density at 2 and 3 wpv. In silico analysis of Tis9 and Tis10 revealed a number of B-cell epitopes in their coil structure, possibly reflecting their immunogenicity. Findings suggested that the combination of Tis9 and Tis10 in DNA and recombinant protein vaccine showed high efficacy for the prevention of TiLV disease in hybrid red tilapia.

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“…Under experimental conditions, Streptococcus spp. thrive at temperatures between 26°C [68] and 37°C [69]. Consequently, outbreaks of contamination primarily occur during the summer, and higher temperatures lead to increased mortality rates [68].…”

Section: Streptococcosismentioning

confidence: 99%

“…thrive at temperatures between 26°C [68] and 37°C [69]. Consequently, outbreaks of contamination primarily occur during the summer, and higher temperatures lead to increased mortality rates [68]. Hu et al [70], in a comparison of S. agalactiae cultures incubated at temperatures of 25°C and 35°C, observed that strains grown at higher temperatures exhibited greater pathogenicity.…”

Section: Streptococcosismentioning

confidence: 99%

Advances in Tilapia farming: Statistical Analysis of the Use of Probiotics and Assessment of their Potentials and Challenges

Pereira,

Reis,

Villasante

et al. 2023

Preprint

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Data regarding the pandemic's impact on tilapia culture remain limited, but it is known that there was a significant decline in production and marketing since 2020. The post-pandemic challenges confronting tilapia farming necessitate prompt solutions, encompassing the management of bacterial infections and the adoption of more advanced technologies by small-scale producers in developing nations. Probiotics, acknowledged as a viable alternative, are presently extensively employed in tilapia aquaculture. Multiple studies have suggested that the application of diverse probiotics in tilapia culture has yielded favorable outcomes. Nonetheless, only a limited number of studies have employed statistical methods to evaluate such findings. To address this gap, a regression analysis was carried out to investigate the existence of a linear relationship between the probiotic dosage added to the feed and two key dependent variables: the specific growth rate (SGR) and the feed conversion ratio (FCR). Additionally, a hierarchical regression analysis was undertaken to ascertain the extent to which the variance observed in these responses could be explained by the variable "probiotic dosage in feed," after accounting for covariates such as initial weight, test duration, water temperature, and number of replicate tanks. Finally, two Pearson correlation matrices were constructed since different studies were included for the SGR and FCR analyses.

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Cytotoxicity of Streptococcus agalactiae secretory protein on tilapia cultured cells

Cited by 6 publications

References 40 publications

Geographical, Temporal and Host-Species Distribution of Potentially Human-Pathogenic Group B Streptococcus in Aquaculture Species in Southeast Asia

Geographical, Temporal and Host-Species Distribution of Potentially Human-Pathogenic Group B Streptococcus in Aquaculture Species in Southeast Asia

Combining segments 9 and 10 in DNA and recombinant protein vaccines conferred superior protection against tilapia lake virus in hybrid red tilapia (oreochromis sp.) compared to single segment vaccines

Advances in Tilapia farming: Statistical Analysis of the Use of Probiotics and Assessment of their Potentials and Challenges

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