Brain Changes and Survival of Animals with Tumors Implanted in the Brain

Abstract

The effects of tumor cells implanted into the brain of animals on survival rates and gross and microscopic brain changes have been ascertained. Walker carcinosarcoma 256 cell suspensions were injected at several brain sites in rats and leukemia L1210 and P388 and Ehrlich ascites tumor cells, intracerebrally into BDF₁ mice. Such neoplasms provided for rapid and rather predictable growth increments. The survival rates were dependent on the number of cells introduced, those receiving the higher counts succumbing first. The brains of the animals revealed fairly discrete space-occupying lesions and neurological symptoms became apparent only hours before death due to intracranial pressure.

Influence of Stress on Lesion Growth and on Survival of Animals Bearing Parenteral and Intracerebral Leukemia L1210 and Walker Tumors

“…Although these lesions are not strictly metastases, they probably do not stem from spillage at the time of the initial injection but may comprise tu mor dislodged to relatively adjacent areas especially on forced exercising and in the earlier phases of the treatment. The study might have profited by the use of lower tumor cell counts by the intramuscular and intracerebral routes where the survival rates are extended in contrast to the lethality of counts ranging as high as 105 cells [5]. In this way, the effects of tumor and addition al stresses of varying intensity might have been discerned more effectively in both intact and adrenalectomized animals.…”

“…An addi tional approach comprised the intracerebral injection of the above tumors and determination of the survival rates in the stressed and control animals. The latter route has been explored recently in relation to brain anatomic changes by this laboratory [5].…”

Cortical Implantation of Hydrocarbons in Rats and Mice

“…The latter were introduced cortically into groups of 14 Holtzman male rats (age: 70 days) under ether anesthesia by trocar as per the technique detailed earlier for tumor fragments (6). The hydrocarbons were introduced intracerebrally into groups of 12 adult male BDF, mice as fine suspensions in saline (1.0 mg/ml), the volume being 0.05 ml.…”

Murine melanoma: a model for intracranial metastasis

“…7 (Rabson et al, 1974;Ausman et al, 1970;Gershbein et al, 1975;Yung et al, 1975;Conley & Remington, 1977;Roseman et at., 1978). Also, intracardiac or intracarotid injections of tumour cells (Ushio et al, 1977;Conley, 1979) require greater technical expertise than i.v.…”

“…The induction of rodent brain tumours by direct intracranial inoculation of tumour cells (Conley & Remington, 1977;Ausman et al, 1970;Gershbein et al, 1975) or virus particles (Rabson et al, 1974;Roseman et al, 1978;Yung et al, 1975) circumvents the need for tumour cells to cross the blood-brain barrier, as they must do in the clinical situation. The use of intracarotid (Ushio et al, 1977) or intracardiac (Conley, 1979) injections of tumour cells has produced experimental brain metastases in rodents, but these techniques require greater dexterity and technical ability than the more simple i.v.…”