Brain cell surface antigens detected by anti-corpus callosum antiserum

Schachner, Melitta; Wortham, Kathleen; Ruberg, Merle; Dorfman, Susan H.; Campbell, Graham LeM.

doi:10.1016/0006-8993(77)90381-x

Cited by 34 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GFAP was first identified in 1971 (Eng et al, 1971) and in mouse appears concomitant with astrocyte differentiation, between 10 to 14 days postnatally (Jacques et al, 1976). GFAP is a major component of the intermediate filaments of astrocytes (Bigmani and Dahl, 1974;Eng and Kasek, 1974;Schachner et al, 1977) and is specific for astrocytes in the CNS and is also found in Schwann cells and satellite cells of the PNS. Studies of tumor cells and normal cells have shown that less well-differentiated astrocytes, contain relatively less GFAP.…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification, and culture of normal mouse colonic glia

Bernstein

Vidrich

1994

Glia

View full text Add to dashboard Cite

We have developed a novel method of isolating and culturing murine colonic mucosal glial cells. Two morphologies are appreciated, a small flat bi or tri polar cell and a larger multipolar cell. The glial cultures have been freed of contaminating fibroblasts and epithelial cells and have been passaged by trypsinization. By intermediate filament (IF) typing, the glial cells have been further characterized as astrocyte-like. All cells expressed glial fibrillary acid protein but not neurofilament 160 protein. The glial cultures expressed the neuropeptides, substance P and substance K. Central nervous system astrocytes synthesize neuropeptides, prostaglandins and cytokines, and can express major histocompatibility class II antigens. It is likely that enteric mucosal glia will also prove to have varied functions. These cultures can now be used to define the role of enteric mucosal glia and to further study their complex interaction with other cells of the colonic mucosa.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation, identification, and culture of normal mouse colonic glia

Bernstein

Vidrich

1994

Glia

View full text Add to dashboard Cite

show abstract

“…Recent methods for the bulk isolation of the three major cell types from brain (2) and the subsequent maintenance of neurons and oligodendroglia (3) have made the production and characterization of these antiserums possible. Our studies differ from previous attempts to study cell markers in which whole regions of brain such as corpus callosum or cerebellum (4) have been used to produce antiserums, as well as subcellular fractions [myelin synaptosomes, or synaptic membranes (5)], cell lines derived from neuronal or glial tumors (6), or soluble brain proteins (7). Of the soluble proteins, the glial fibrillary acidic protein does seem specific for astroglial cytoplasm (8).…”

mentioning

confidence: 68%

“…370 (1974)]. Our analysis suggests that the opposite is true; for more detailed refutation of Lynch and Johnson's claim, see (4). 14.…”

mentioning

confidence: 75%

See 1 more Smart Citation

Antiserums to Neurons and to Oligodendroglia from Mammalian Brain

Poduslo

McFarland

McKhann

1977

Science

View full text Add to dashboard Cite

show abstract

“…A sonicate of fresh bovine corpus callosum was prepared according to Schachner et al [1977]. Female BALB/c mice, 8-10 weeks of age (Charles River), were immunized with an intraperitoneal injection of 0.5 mg and Yu, 19831.…”

Section: Methods and Materials Antibody Productionmentioning

confidence: 99%

Myelin‐specific domain on the plasmalemma of oligodendroglia: Differential expression in the rat and hypomyelinating mouse mutants jimpy and quaking

Gard

Dutton

1987

J of Neuroscience Research

View full text Add to dashboard Cite

Monoclonal antibody, 1A9, prepared against bovine white matter, recognizes a proteinaceous, myelin-specific domain in the CNS that is restricted to the surface of oligodendroglia in primary dissociated cell cultures. The antigen is not detected in the PNS or non-neural tissues. Antibody binding is abolished by heating, exposure to SDS and delipidation, indicating that a conformationally sensitive epitope is recognized. The antigen is present in tracts of developing white matter in rat cerebellum beginning at 5 days postnatally. In developing cultures of fetal rat brain the period of rapid onset for the phenotypic expression of 1A9 antigen is similar to that of galactocerebroside, corresponding to 2-4 postnatal days of age. The 1A9 antigen is not observed in white matter or cultured oligodendroglia of the hypomyelinating jimpy mutant mouse, but its expression is qualitatively normal in the quaking mutant. The possibility is raised that 1A9 may be the primary target of the jimpy mutation.

show abstract

Brain cell surface antigens detected by anti-corpus callosum antiserum

Cited by 34 publications

References 19 publications

Isolation, identification, and culture of normal mouse colonic glia

Isolation, identification, and culture of normal mouse colonic glia

Antiserums to Neurons and to Oligodendroglia from Mammalian Brain

Myelin‐specific domain on the plasmalemma of oligodendroglia: Differential expression in the rat and hypomyelinating mouse mutants jimpy and quaking

Contact Info

Product

Resources

About