Brachypodium distachyon as a new model system for understanding iron homeostasis in grasses: phylogenetic and expression analysis of Yellow Stripe-Like (YSL) transporters

Yordem, Burcu Kokturk; Conte, Sarah; Feng, Jian; Yokosho, Kengo; Vasques, Kenneth A.; Gopalsamy, Srinivasa Nithin; Walker, Elsbeth L.

doi:10.1093/aob/mcr200

Cited by 57 publications

(42 citation statements)

References 49 publications

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“…This has led to the development of new model systems, closer to species of economic relevance for humans. For temperate poaceous crops like wheat and barley, the small, fast growing grass Brachypodium distachyon has become a promising model organism (Mur et al, 2011; Brkljacic et al, 2011; Vogel and Hill, 2008; Huo et al, 2008; Yordem et al, 2011; Garvin, 2008; Draper et al, 2001). The intrinsic properties of B. distachyon , including self-fertility, its short life cycle, a small sequenced diploid genome, and its genetic accessibility, make it highly suitable as a laboratory model plant.…”

Section: Introductionmentioning

confidence: 99%

A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem

Rabe

Bosch

Stirnberg

et al. 2016

eLife

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Due to their economic relevance, the study of plant pathogen interactions is of importance. However, elucidating these interactions and their underlying molecular mechanisms remains challenging since both host and pathogen need to be fully genetically accessible organisms. Here we present milestones in the establishment of a new biotrophic model pathosystem: Ustilago bromivora and Brachypodium sp. We provide a complete toolset, including an annotated fungal genome and methods for genetic manipulation of the fungus and its host plant. This toolset will enable researchers to easily study biotrophic interactions at the molecular level on both the pathogen and the host side. Moreover, our research on the fungal life cycle revealed a mating type bias phenomenon. U. bromivora harbors a haplo-lethal allele that is linked to one mating type region. As a result, the identified mating type bias strongly promotes inbreeding, which we consider to be a potential speciation driver.DOI: http://dx.doi.org/10.7554/eLife.20522.001

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Section: Introductionmentioning

confidence: 99%

A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem

Rabe

Bosch

Stirnberg

et al. 2016

eLife

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“…Now that several plant genomes have been sequenced, it is clear that higher plants possess four distinct, well-conserved groups of YSL proteins, and that one of these is unique to grass species (Curie et al, 2009; Yordem et al, 2011). Substantial progress has been made in understanding the roles of the YSLs that are most closely related to ZmYS1 [e.g., AtYSL1, AtYSL2 and AtYSL3 (DiDonato et al, 2004; Waters et al, 2006; Chu et al, 2010) and OsYSL2 (Koike et al, 2004; Inoue et al, 2006; Ishimaru et al, 2010)], but there is little information concerning members of the other two conserved YSL clades.…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis thaliana Yellow Stripe1-Like4 and Yellow Stripe1-Like6 localize to internal cellular membranes and are involved in metal ion homeostasis

Ss¹,

Hh²,

Dc³

et al. 2013

Front. Plant Sci.

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Several members of the Yellow Stripe1-Like (YSL) family of transporter proteins are able to transport metal-nicotianamine (NA) complexes. Substantial progress has been made in understanding the roles of the Arabidopsis YSLs that are most closely related to the founding member of the family, ZmYS1 (e.g., AtYSL1, AtYSL2 and AtYSL3), but there is little information concerning members of the other two well-conserved YSL clades. Here, we provide evidence that AtYSL4 and AtYSL6, which are the only genes in Arabidopsis belong to YSL Group II, are localized to vacuole membranes and to internal membranes resembling endoplasmic reticulum. Both single and double mutants for YSL4 and YSL6 were rigorously analyzed, and have surprisingly mild phenotypes, in spite of the strong and wide-ranging expression of YSL6. However, in the presence of toxic levels of Mn and Ni, plants with mutations in YSL4 and YSL6 and plants overexpressing GFP-tagged YSL6 showed growth defects, indicating a role for these transporters in heavy metal stress responses.

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“…Supplemental light was supplied with high-pressure sodium lamps to give a 20 h light period each day. For quantitative polymerase-chain-reaction (PCR) analysis, plants were grown in a sand:Turface mix (9:1 v/v) irrigated with water until germination and then irrigated with modified Hoagland’s nutrient solutions, with 1 mM KH 2 PO 4 , 3.75 mM KOAc, 5 mM Ca(NO 3 ) 2 , 1.25 mM KNO 3 , 2 mM MgSO 4 , 3.75 mM NH 4 OAc, 46 uM H 3 BO 3 , 9.1 uM MnCl 2 , 0.77 uM ZnSO 4 , 0.32 uM CuSO 4 , and 0.83 uM H 2 MoO 4 (Yordem et al, 2011) containing 100 μM FeSO 4 -EDTA every 48 h. Plants were grown for 10 days after germination before the root tissue was collected.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Yellow Striped Mutants of Zea mays Reveals Novel Loci Contributing to Iron Deficiency Chlorosis

Chan-Rodriguez

Walker

2018

Front. Plant Sci.

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The micronutrient iron (Fe) is essential for photosynthesis, respiration, and many other processes, but it is only sparingly soluble in aqueous solution, making adequate acquisition by plants a serious challenge. Fe is a limiting factor for plant growth on approximately 30% of the world’s arable lands. Moreover, Fe deficiency in humans is a global health issue, affecting 1.62 billion people, or about 25% of the world’s population. It is imperative that we gain a better understanding of the mechanisms that plants use to regulate iron homeostasis, since these will be important targets for future biofortification and crop improvement strategies. Grasses and non-grasses have evolved independent mechanisms for primary iron uptake from the soil. The grasses, which include most of the world’s staple grains, have evolved a distinct ‘chelation’ mechanism to acquire iron from the soil. Strong iron chelators called phytosiderophores (PSs) are synthesized by grasses and secreted into the rhizosphere where they bind and solubilize Fe(III). The Fe(III)-PS complex is then taken up into root cells via transporters specific for the Fe(III)-PS complex. In this study, 31 novel, uncharacterized striped maize mutants available through the Maize Genetics Cooperation Stock Center (MGCSC) were analyzed to determine whether their mutant phenotypes are caused by decreased iron. Many of these proved to be either pale yellow or white striped mutants. Complementation tests were performed by crossing the MGCSC mutants to ys1 and ys3 reference mutants. This allowed assignment of 10 ys1 alleles and 4 ys3 alleles among the novel mutants. In addition, four ys∗ mutant lines were identified that are not allelic to either ys1 or ys3. Three of these were characterized as being non-allelic to each other and as having low iron in leaves. These represent new genes involved in iron acquisition by maize, and future cloning of these genes may reveal novel aspects of the grass iron acquisition mechanism.

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Brachypodium distachyon as a new model system for understanding iron homeostasis in grasses: phylogenetic and expression analysis of Yellow Stripe-Like (YSL) transporters

Cited by 57 publications

References 49 publications

A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem

A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem

Arabidopsis thaliana Yellow Stripe1-Like4 and Yellow Stripe1-Like6 localize to internal cellular membranes and are involved in metal ion homeostasis

Analysis of Yellow Striped Mutants of Zea mays Reveals Novel Loci Contributing to Iron Deficiency Chlorosis

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