BPA activates EGFR and ERK1/2 through PPARγ to increase expression of steroidogenic acute regulatory protein in human cumulus granulosa cells

Pogrmić-Majkić, Kristina; Nenadov, Dragana Samardzija; Fa, Svetlana; Stanić, Bojana; Pjevic, Aleksandra Trninic; Andrić, Nebojša

doi:10.1016/j.chemosphere.2019.04.174

Cited by 31 publications

(13 citation statements)

References 42 publications

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“…Indeed, granulosa cells exposed to BPS at similar concentrations to those used in this study (up to 10 µM) did not exhibit reduced progesterone secretion in swine [61] or bovines [62]. Concerning BPA effects, a study reported that 100 µM BPA decreased progesterone production by FSH-stimulated porcine COCs after 44 h culture [23] whereas in human cumulus granulosa cells the same concentration of BPA increased progesterone secretion after 48 h culture [63]. There are also consistent reports of decreases in progesterone secretion after porcine granulosa cells are exposed to BPA [20,22,64].…”

Section: Bps Reduced CC Progesterone Secretionsupporting

confidence: 45%

Bisphenol S Impaired In Vitro Ovine Early Developmental Oocyte Competence

Desmarchais

Têteau

Papillier

et al. 2020

IJMS

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Introduction: Bisphenol A (BPA) is a widespread compound in the plastic industry that is especially used to produce baby bottles, food packaging and metal cans. BPA, an endocrine disruptor, leads to alterations in reproductive function and therefore has been banned from the food industry. Unregulated BPA analogues, particularly Bisphenol S (BPS), have emerged and are now used in the plastic industry. Thus, this study aimed to examine the acute effects of low and environmental doses of BPS on ewe oocyte quality and developmental competence, and its mechanism of action, during in vitro maturation. Methods: Ewe cumulus-oocyte complexes underwent in vitro maturation in the presence or absence of BPS (1 nM, 10 nM, 100 nM, 1 µM or 10 µM). Oocytes were then subjected to in vitro fertilisation and development. Results: 1 µM BPS induced a 12.7% decrease in the cleavage rate (p = 0.004) and a 42.6% decrease in the blastocyst rate (p = 0.017) compared to control. The blastocyst rate reduction was also observed with 10 nM BPS. Furthermore, 10 µM BPS reduced the oocyte maturation rate, and 1 µM BPS decreased cumulus cell progesterone secretion. PR and AMH gene expression were reduced in cumulus cells. BPS induced a 5-fold increase in MAPK 3/1 activation (p = 0.04). Conclusions: BPS impaired ewe oocyte developmental competence. The data suggest that BPS might not be a safe BPA analogue. Further studies are required to elucidate its detailed mechanism of action.

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Section: Bps Reduced CC Progesterone Secretionsupporting

confidence: 45%

Bisphenol S Impaired In Vitro Ovine Early Developmental Oocyte Competence

Desmarchais

Têteau

Papillier

et al. 2020

IJMS

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“…The molecular mechanisms by which BPA increases STAR expression has not been fully clarified. Pogrmic‐Majkic et al demonstrated that increased expression of STAR may be mediated through the PPARγ/EGFR/ERK1/2 pathway in human cumulus granulosa cells (Pogrmic‐Majkic et al, 2019). Medwid et al found that BPA may increase STAR protein expression via an ER‐mediated novel molecular in H295A adrenal cortical cells (Medwid, Guan, & Yang, 2018).…”

Section: Discussionmentioning

confidence: 99%

Bisphenol A decreases progesterone synthesis in human ovarian granulosa cells

Liu

Yang

et al. 2020

Birth Defects Research

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Evidences indicate that the decline of female fertility is becoming a common problem over the past few decades. Environmental exposure of Bisphenol A (BPA) has been considered as an endocrine-disrupting chemical deleteriously affecting human reproductions. To better understand the effect of BPA exposure on human ovarian granulosa cells, we treated human ovarian granulosa cell line (KGN) with increasing concentrations (0.1, 1, 10, and 100 μM) of BPA for 24 hr. About 0.1, 1, and 10 μM BPA did not significantly affect the viability of KGN while 100 μM of BPA caused a statistically significant decrease in the viability of KGN. Treatment KGN with 10 μM BPA resulted in a significant decrease in progesterone biosynthesis. The treatment also significantly increased the expression of ATP binding cassette subfamily A member 1 (ABCA1) and steroidogenic acute regulatory protein (STAR). In the current study, exposure to BPA could lead to decreased progesterone production probably through the upregulation of ABCA1 in human granulosa cells.

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“…Studies using the structural analog of BPA, bisphenol S (BPS), reported a similar decrease in progesterone production and CYP11A1 expression when luteinized GCs isolated from women undergoing IVF treatment were exposed to 10 or 50 μM BPS 98 . Conversely, BPA at higher concentrations had the opposite effect on progesterone production in cultured human cumulus GCs, which reflect the properties of GCs in the early antral follicle 99 . This study determined that BPA increased progesterone production via an EGFR-and ERK1/2-mediated rise in STAR transcript levels and suggests that the effect of BPA on progesterone production can depend on BPA concentration and the differentiation status of GCs.…”

Section: Bisphenolsmentioning

confidence: 87%

Updates on molecular and environmental determinants of luteal progesterone production

DeWitt

Whirledge

Kallen

2020

Molecular and Cellular Endocrinology

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Progesterone, a critical hormone in reproduction, is a key sex steroid in the establishment and maintenance of early pregnancy and serves as an intermediary for synthesis of other steroid hormones. Progesterone production from the corpus luteum is a tightly regulated process which is stimulated and maintained by multiple factors, both systemic and local. Multiple regulatory systems, including classic mediators of gonadotropin stimulation such as the cAMP/PKA pathway and TGFβ-mediated signaling pathways, as well as local production of hormonal factors, exist to promote granulosa cell function and physiological fine-tuning of progesterone levels. In this manuscript, we provide an updated narrative review of the known mediators of human luteal progesterone and highlight new observations regarding this important process, focusing on studies published within the last five years. We will also review recent evidence suggesting that this complex system of progesterone production is sensitive to disruption by exogenous environmental chemicals that can mimic or interfere with the activities of endogenous hormones.

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BPA activates EGFR and ERK1/2 through PPARγ to increase expression of steroidogenic acute regulatory protein in human cumulus granulosa cells

Cited by 31 publications

References 42 publications

Bisphenol S Impaired In Vitro Ovine Early Developmental Oocyte Competence

Bisphenol S Impaired In Vitro Ovine Early Developmental Oocyte Competence

Bisphenol A decreases progesterone synthesis in human ovarian granulosa cells

Updates on molecular and environmental determinants of luteal progesterone production

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