Box-Behnken design to study the bergenin content and antioxidant activity of Endopleura uchi bark extracts obtained by dynamic maceration

Abstract

Brazil has one of the world's largest biodiversity in flora and a plant that has attracted attention is the Endopleura uchi (Huber) Cuatrec., Humiriaceae, which is native of the Brazilian Amazon. Among the many popular uses, this species is utilized in the treatment of woman's genito urinary tract affections and also as anti-inflammatory. It is believed that their actions derive from the major constituent, bergenin. The objective of this work was to study the Endopleura uchi barks extraction using the dynamic maceration method and the effects of the extraction time, drug to solvent ratio and temperature. A Box-Behnken design was applied to study the influence of these factors and the respective response surfaces. The extract characterization was made by determination of its antioxidant activity by DPPH; total polyphenol content and bergenin content. In general, the extracts showed good antioxidant activity, with the IC50 ranging from 4.02 to 5.87 µg/mL. The polyphenol content ranged from 31.89 to 47.82%. High levels of chemical markers are observed in all extracts, with average bergenin content of 35.58%. The result showed that the multivariate study of extraction is key step in the development and standardization of extracts Endopleura uchi

Ellagic Acid and Derivatives from Cochlospermum angolensis Welw. Extracts: HPLC–DAD–ESI/MSn Profiling, Quantification and In Vitro Anti‐depressant, Anti‐cholinesterase and Anti‐oxidant Activities

“…Two main reasons can be indicated. First, the stirring process increases the contact between the plant material and the solvent, leading to an increase of solvent molecules around solid particles (Tacon and Freitas, ). Second, the sonication can disrupt cell walls and act as a solvent micropump that forces the solvent into the cell to dissolve the compounds (Vinatoru, ; Albu et al ., ).…”

Endopleura uchi (Huber) Cuatrec, a medicinal plant with potential anti-inflammatory activity: A review of its phytochemistry and biological activities

“…Through dynamic maceration under controlled period of extraction, temperature, and solvent concentration (ethanol), Tacon and Freitas (2013) improved the extraction of polyphenols between 31.89 and 47.82%, and bergenin in 35.58% compared to the extract weight and in 4% compared to barks weigh (Tacon and Freitas, 2013).…”

“…Moreover, using an ethanol extract from barks through dynamic maceration, Tacon and Freitas (2013) reported a significant antioxidant activity in the DPPH assay (IC 50 values from 4.02 to 5.87 µg/mL). The authors attributed this antioxidant activity to the high content of polyphenols and bergenin from the extract.…”

Caracterização da pele fotoenvelhecida, desenvolvimento e eficácia clínica de formulações dermocosméticas por técnicas de biofísica e análise de imagem

“…Nesse contexto, técnicas estatísticas de planejamento experimental vêm sendo utilizadas em diversas áreas do conhecimento para a otimização de processos e produtos com a finalidade de encontrar as condições ótimas e obtenção de um produto com as características desejadas (COSTA-MACHADO; BASTOS; DE FREITAS, 2013;TACON;.…”

“…Considerando a importância da composição adequada da formulação no desenvolvimento de produtos cosméticos estáveis, seguros e eficazes, o planejamento experimental é uma ferramenta de grande valia uma vez que possibilita a análise de muitas variáveis (fatores) de um processo produtivo, e permite obter um número maior de informações utilizando um menor número de experimentos (COSTA-MACHADO; BASTOS; DE FREITAS, 2013;FERREIRA et al, 2007;TACON; Este tipo de planejamento normalmente é representado por (Xn), sendo que "n" representa o número de fatores e "X" o número de níveis escolhidos. Assim, são determinados valores para os fatores em estudo, chamados de níveis codificados como -1, 0 e 1, que correspondem aos níveis baixo, intermediário e alto, respectivamente (DE BARROS NETO;SCARMINIO;BRUNS, 2003;PEREIRA, 2015).…”