Bovine Serum Albumin-Immobilized Black Phosphorus-Based γ-Fe2O3 Nanocomposites: A Promising Biocompatible Nanoplatform

Abstract

The interactions between proteins and nanoparticles need to be fully characterized as the immobilization of proteins onto various nanoplatforms in the physiological system often results in the change of surface of the protein molecules to avoid any detrimental issues related to their biomedical applications. Hence, in this article, the successful low-temperature synthesis of a BP-based γ-Fe2O3 (IB) nanocomposite and its interactive behavior with bovine serum albumin (BSA)—a molecule with chemical similarity and high sequence identity to human serum albumin—are described. To confirm the formation of γ-Fe2O3 and the IB nanocomposite, X-ray diffraction, transmission electron microscopy, and X-ray photoelectron spectroscopy analyses of the materials were performed. Additionally, the physical interaction between BSA and the IB nanocomposite was confirmed via UV–Vis and photoluminescence spectral analyses. Finally, the biocompatibility of the BSA-immobilized IB nanocomposite was verified using an in vitro cytotoxicity assay with HCT-15 colon cancer cells. Our findings demonstrate that this newly developed nanocomposite has potential utility as a biocompatible nanoplatform for various biomedical applications.

Effect of Ionic and Non-Ionic Surfactant on Bovine Serum Albumin Encapsulation and Biological Properties of Emulsion-Electrospun Fibers

“…Proteins and peptides are of significant interest among them, not only due to their impact on tissue regeneration (as they are a basic component of the cells) but also for their role in enzymatic catalysis, metabolic processes, signal transduction, and immune response. Human serum albumin (HSA) is one of the most abundant proteins in human plasma that performs dozens of important functions, including the transport of amino acids, fatty acids, steroids, and drugs; regulation of pH and oncotic blood pressure [ 5 , 6 , 7 ]. It also removes harmful factors for the cell’s survival, such as reactive oxygen species, and protects them against lysis (in low concentrations such as 1 g/L), making it an excellent molecule for both in vitro (maintaining the eucaryotic cell cultures) and in vivo conditions.…”

“…Due to the resemblance of the structure to HSA, bovine serum albumin (BSA) is one of the most used model proteins for studying the reaction of the human organism to biomaterials. It can be used both as the ion or drug carrier as well as the modifier itself (e.g., in a sustained delivery system for promoting early adhesion of the human gingival fibroblasts) [ 5 , 6 ].…”

Adsorption characteristics of bovine serum albumin onto α-Fe2O3 nanoparticles prepared via the alcohol solution combustion process of ferric nitrate

“…The infrared spectrum of magnetic α-Fe 2 O 3 nanoparticles, BSA, post-adsorption magnetic α-Fe 2 O 3 nanoparticles and re-calcined magnetic α-Fe 2 O 3 nanoparticles were shown in figure 6. Compared figure 6(b) with figure 6(c), the peak at 1654 cm −1 was the characteristic peak of carbonyl stretching vibration of the amide I bond and 1540 cm −1 was the characteristic peak of N-H bending and C-N stretching of the amide II bond, the peak at 2923 cm −1 was the characteristic peak of CH 2 , the peaks at 551 cm −1 and 472 cm −1 were the characteristic peak of the Fe-O bond, indicating that the adsorption of BSA onto magnetic α-Fe 2 O 3 nanoparticles [37,[53][54][55][56]. Compared figure 6(c) with figure 6(d), it was obvious that BSA had been removed from magnetic α-Fe 2 O 3 nanoparticles after re-calcination.…”

“…In this project, we prepared magnetic α-Fe 2 O 3 nanoparticles via the ethanol solution of iron nitrate combustion-calcination process, which would provide research basis for the construction of drug delivery system in living organisms; and with bovine serum albumin (BSA) [37] as the model of biological macromolecule due to its representativeness, low price and easy purification [38,39], the adsorption capacity and mechanism of BSA onto α-Fe 2 O 3 nanoparticles were revealed.…”

Biomimetic Nanoparticles Coated with Bacterial Outer Membrane Vesicles as a New-Generation Platform for Biomedical Applications

“…Bovine serum albumin (BSA) is another NP used in inner core materials due to its high availability and inexpensive cost [94]. In the presence of reactive groups such as thiol and amine, it is extremely water-soluble and flexible, allowing it to interact with other compounds such as drugs and inorganic materials for use in drug delivery systems by allowing drugs to be transported in the body through regulating drug encapsulation.…”