Bovine oocyte maturation, fertilization and further development in vitro and after transfer into recipients

Prokofiev, M. I.; Ernst, L.K.; Сураева, Н. М.; Lagutina, Irina; Udavlennikova, N.N.; Kesyan, A.Z.; Dolgohatskiy, A.I.

doi:10.1016/0093-691x(92)90065-y

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…The results from this study show that embryo production from OPU-derived COCs is not affected when oocytes have been matured in vitro between 16 and 28 h. (Prokofiev et al 1992) and 19-24 h (Merton et al 2003). Independent of the variation in optimal maturation culture periods as described previously, in almost all studies a relative large drop in embryo production rate was seen when the optimal maturation culture period was exceeded.…”

Section: Embryo Productionsupporting

confidence: 64%

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Bovine OPU‐Derived Oocytes can be Matured In Vitro for 16–28 h with Similar Developmental Capacity

Merton

Roos

Koenen

et al. 2012

Reprod Domestic Animals

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The aim of this study was to determine the optimal maturation culture period of ovum pick up (OPU)-derived cumulus oocytes complexes (COCs) in relation to their developmental capacity. Embryo production, embryo cryotolerance, post-transfer embryonic survival and calf characteristics such as gestation length, birthweight and sex ratio were investigated. This retrospective study covers the analyses of ovum pick up -in vitro production and calving results from a commercial programme that took place between March 1994 and September 2004. Donors were both heifers (of which approximately 90% pregnant) and cows (of which approximately 10% pregnant). Embryo production analyses were based on 7800 OPU sessions conducted from January 1995 until January 1999. Analyses of calving rate were based on 13 468 embryo transfers performed during January 1995 until May 2002. Analyses on calf characteristics were based on 2162 calves born between March 1994 and September 2004. The in vitro maturation culture period ranged from 16 to 28 h. The mean production rate of transferable embryos was 16.5% (1.2 embryos per OPU session). Length of maturation culture period did not affect the production of transferable embryos. Mean calving rate was 40.9% and 38.7% for fresh and frozen/thawed embryos, respectively. Calving rate was not affected by the maturation culture period. Mean birthweight, gestation length and proportion of male calves were 46 kg, 281.9 days and 52.8%, respectively. Maturation culture period did not affect these variables. In conclusion, this study shows that the in vitro maturation culture period within the range of 16-28 h does not affect in vitro embryo production, embryo cryotolerance, post-transfer embryonic survival and calf characteristics, suggesting that all COC batches collected by OPU on the same day, can be fertilized in one IVF session without a significant loss in the production from oocyte to calf.

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Section: Embryo Productionsupporting

confidence: 64%

“…2005), 22 h (Agung et al. 2006), 24 h (Tarin and Gomez‐Piquer 2002), 18–24 h (Prokofiev et al. 1992) and 19–24 h (Merton et al.…”

Section: Discussionmentioning

confidence: 99%

Bovine OPU‐Derived Oocytes can be Matured In Vitro for 16–28 h with Similar Developmental Capacity

Merton

Roos

Koenen

et al. 2012

Reprod Domestic Animals

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“…Good incubator will be able to distribute and regulate the concentration of CO 2 permanently and evenly, and the temperature is not too much changed around 38°C. Incubation in medium TCM 199 for 18 and 24 hours there was no significant difference in the numbers of maturation, respectively 71% and 76% (Prokofiev et al 1992).…”

Section: Introductionmentioning

confidence: 82%

Fertilization of Bovine Oocytes Vitrified Pre- And Post in Vitro Maturation

Faizah¹,

Darsini²,

Hinting³

2017

FMI

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The success rate of fertilization post save frozen oocytes is still very low, because the oocyte has distinctive features, namely the volume ratio and a lower surface to the limited penetration of water and cryoprotectants penetrate cells. Beside mature oocytes have a thread spindles are particularly vulnerable to the drop in temperature. Keep frozen oocytes is needed, especially in women who needed rescue fertility so their oosit can be fertilized. Maturation is done in TC 100 mL medium covered with mineral oil in a petri dish with a diameter of 36 mm. Oocyte vitrification begins with washing in PBS supplemented medium serum 20% for 1-2 minutes, followed by serum in the medium PBS + 20% + 10% ethylene glycol for 10-14 minutes. Then oocyte vitrification medium is transported in PBS + serum 20% + sucrose 0.5M ethylene glycol + 15% + 15% PROH for 25-30 seconds. Thawing oocytes is done by successive immersed in the media: 1). PBS + 20% serum + 0.5M sucrose, 2). PBS + 20% serum + 0.25M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose. Insemination is done in rosset, and the number of fertilization was observed after 48 hours. Fertilization in the control group amounted to 42.97%, while the K1 and K2 there are no fertilization at all. The analysis showed that fertilization in the control and treatment groups significantly different at p <0.05 in both treatment groups K1 or K2 there are no fertilization at all. The conclusions of this study is there is no difference between the amount of fertilization of bovine oocytes were vitrified pre and post-maturation in vitro.

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Development of bovine embryos in a cell-free culture medium: Effects of type of serum, timing of its inclusion and heat inactivation

1994

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Bovine oocyte maturation, fertilization and further development in vitro and after transfer into recipients

Cited by 7 publications

References 10 publications

Bovine OPU‐Derived Oocytes can be Matured In Vitro for 16–28 h with Similar Developmental Capacity

Bovine OPU‐Derived Oocytes can be Matured In Vitro for 16–28 h with Similar Developmental Capacity

Fertilization of Bovine Oocytes Vitrified Pre- And Post in Vitro Maturation

Development of bovine embryos in a cell-free culture medium: Effects of type of serum, timing of its inclusion and heat inactivation

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