Bovine Mastitis: Prevalence, Risk Factors, and Bacterial Pathogens Isolated in Lactating Cows in Gamo Zone, Southern Ethiopia

Abstract

Purpose Mastitis is a disease known to cause a great deal of loss of production and has a major economic impact. In the study area, there is little current information on bovine mastitis. Therefore, this study aimed to determine the overall prevalence of bovine mastitis and its associated risk factors and isolate the major pathogenic bacteria. Methods A cross-sectional study was conducted from February 2020 to September 2020 in selected dairy farms of Gamo Zone, southern Ethiopia. A total of 422 lactating cows were diagnosed for mastitis using the California mastitis test, clinical examination, and bacteriological methods. Results The overall prevalence of bovine mastitis determined in the area was 17.1% (72 of 422), of which 1.9% (eight of 422) was clinical and 15.2% (64 of 422) subclinical. Of 1,662 quarters examined, 7.94% (132) were positive. Bacteriological methods were also used to isolate the major pathogenic bacterial species associated with bovine mastitis. From 72 composite milk samples, growth of six different groups of bacteria was recorded in 64 (88.9%) samples. The most predominant bacterial pathogens isolated were Staphylococcus aureus (42.6%), ahead of Streptococcus spp. (26.2%), non-aureus staphylococci (14.8%), and Escherichia coli (11.5%). Salmonella spp. (3.3%) and Klebsiella pneumoniae (1.6%) were the least isolated bacterial pathogens. Among risk factors, breed, parity, udder depth, and tick infestation of the udder showed statistically significant differences ( P <0.05) regarding the occurrence of mastitis. Conclusion The current study revealed that mastitis is one of the health problems affecting dairy cows in Gamo. Enhancing the awareness of dairy farmers, regular screening, and improving hygienic conditions are critically important to control and prevent bovine mastitis in the study area.

WITHDRAWN: Study On Prevalence Of Bovine Mastitis, Associated Risk Factors, And Isolation Of Staphylococcus Species And Escherichia Coli From Mastitic Milk In Dairy Farms Of Wolaita Sodo Town And Its Suburbs, Southern Ethiopia

“…Harerghe zone, Doba district, Ethiopia (23.18%) [33], Egypt (19.14%) [34], and Gamo zone, Southern Ethiopia (17.1%) [35], In contrast, the present prevalence was lower than a previous report in Bishoftu town (40.1%) [36], in Mogadishu, Somalia (44.5%) [37], in and around Haramaya (49.2%) [38] in Rwanda (50.4%) [39], and in Southern Ethiopia (54.2%) [9].…”

Prevalence of Bovine Mastitis and Its Associated Risk Factors among Dairy Cows in Ethiopia during 2005–2022: A Systematic Review and Meta-Analysis

“…The subclinical mastitis in this study was32.21%, which is closely in agreement with the findings of 31.67% in Gondar District [ 35 ], 32.20% in Addis Ababa [ 81 ], 32.8% in Ambo District [ 82 ], 33.8% in Holeta District [ 83 ], 34.30% in and around Addis Ababa [ 84 ], and 36.67% in Sebeta District [ 85 ]. The current finding was higher than the reports of 15.2% in Gamo Zone [ 86 ], 23.10% in Wolaita Sodo [ 2 ], and 25.2% in Bahir Dar and its environs [ 77 ]. However, the subclinical mastitis recorded in this study was lower than the previous findings of 55.1% in and around Addis Ababa [ 76 ], 69.8% in Addis Ababa and its vicinity [ 84 ], 70.62% around Addis Ababa [ 43 ], 74% in Kenya [ 73 ], 76% in Hawassa [ 37 ], 76.2% in Rwanda [ 72 ], and 85% in Jimma [ 87 ].…”

Bta-miR-223 Targeting the RHOB Gene in Dairy Cows Attenuates LPS-Induced Inflammatory Responses in Mammary Epithelial Cells

“…Once pathogenic bacteria have entered the breast, bMECs respond rapidly to initiate the antimicrobial response [30,31]. Interestingly, mRNA levels of all inflammatory factors increased rapidly 2-4 h after stimulation of bMECs with LPS and LTA, but only LPS stimulation resulted in sustained inflammatory factor expression [19,32] of the TLR4/NF-κB inflammatory signaling pathway [33].…”