Bovine mammary gene expression profiling using a cDNA microarray enhanced for mammary-specific transcripts

Abstract

A cDNA microarray resource enhanced for transcripts specific to the bovine mammary gland (BMAM) has been developed and used in pilot studies to examine gene expression profiles in the mammary gland. One goal driving development of this resource was to shed some light on the pathways and mechanisms specifically related to bovine mammary gland growth and development. To accomplish this, gene expression patterns from bovine adipose, liver, adrenal, lymph, spleen, thymus, gut, and developing mammary tissue were compared using the BMAM microarray. We have thus identified a putative set of 16 genes being preferentially expressed in developing mammary gland. Another of our long-term goals is to elucidate the genes and pathways associated with bovine lactation and involution and to use these as a model for human mammary gland development as it relates to human breast cancer risks. To begin this process, we conducted a pilot study, comparing gene expression profiles of lactating bovine mammary tissue against nonlactating tissue on the BMAM microarray. Our results have yielded many novel and interesting genes exhibiting differential expression in lactating mammary tissue, including oncogenes (VAV3, C-myc), mediators of apoptosis (Caspase 8), and cell cycle regulators (LASP1).

Evidence for a Novel Gene Expression Program in Peripheral Blood Mononuclear Cells from Mycobacterium avium subsp. paratuberculosis -Infected Cattle

“…Previous studies with similar cDNA microarrays demonstrated that with these selection criteria, the expected number of false positives (genes showing differential expression when none is expected) is almost zero (37). Thus, it is likely that most gene expression differences identified by using these criteria in a cDNA microarray analysis are valid.…”

Novel family- and genus-specific DNA markers in Mugilidae

“…The 18S ribosomal gene was used as an internal control (18S-F: 5'-ctcccctcccgttacttgga-3' and 18S-R: 5'-ttggttttggtctgataaatgca-3') (Suchyta et al, 2003). The PCR conditions were the same as those used for RAPD-PCR, while the annealing temperature was raised to 62°C.…”

Examination of animal and zoonotic pathogens using microarrays

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