Bovine leukemia virus matrix-associated protein MA(p15): further processing and formation of a specific complex with the dimer of the 5'-terminal genomic RNA fragment

Katoh, Iyoko; Kyushiki, Hiroyuki; Sakamoto, Yoko; Ikawa, Yoji; Yoshinaka, Yoshiyuki

doi:10.1128/jvi.65.12.6845-6855.1991

Cited by 57 publications

(44 citation statements)

References 42 publications

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“…This is similar to the destabilization observed throughout the HIV-1 5Ј leader upon HIV-1 Gag binding as elucidated by XL-SHAPE (48). BLV MA-gRNA interaction sites have previously been mapped to regions spanning the PBS to the 5Ј-terminal 60 residues in the gag coding region (29,79), which are very similar to the probing results for HTLV-1 MA reported here.…”

Section: Rna-binding Specificity Of Htlv-1 Gag Domainssupporting

confidence: 83%

“…It has been suggested that HIV-1 MA may play a supplementary role in gRNA packaging, likely by binding outside ⌿ regions (28). Whereas BLV NC exhibited nonspecific RNAbinding activity, BLV MA forms a highly specific complex with the 5Ј end of the gRNA dimer but not with other RNAs in vitro, suggesting that MA plays a more central role in gRNA selection than NC (29). Consistent with this observation, BLV MA binds to two SL RNAs derived from the BLV packaging signal with high affinity (10 -20 nM) (30).…”

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confidence: 99%

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Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization

Hatterschide

Syu

et al. 2018

Journal of Biological Chemistry

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Human T-cell leukemia virus type 1 (HTLV-1) is the first retrovirus that has conclusively been shown to cause human diseases. In HIV-1, specific interactions between the nucleocapsid (NC) domain of the Gag protein and genomic RNA (gRNA) mediate gRNA dimerization and selective packaging; however, the mechanism for gRNA packaging in HTLV-1, a deltaretrovirus, is unclear. In other deltaretroviruses, the matrix (MA) and NC domains of Gag are both involved in gRNA packaging, but MA binds nucleic acids with higher affinity and has more robust chaperone activity, suggesting that this domain may play a primary role. Here, we show that the MA domain of HTLV-1, but not the NC domain, binds short hairpin RNAs derived from the putative gRNA packaging signal. RNA probing of the HTLV-1 5' leader and cross-linking studies revealed that the primer-binding site and a region within the putative packaging signal form stable hairpins that interact with MA. In addition to a previously identified palindromic dimerization initiation site (DIS), we identified a new DIS in HTLV-1 gRNA and found that both palindromic sequences bind specifically the NC domain. Surprisingly, a mutant partially defective in dimer formation exhibited a significant increase in RNA packaging into HTLV-1-like particles, suggesting that efficient RNA dimerization may not be strictly required for RNA packaging in HTLV-1. Moreover, the lifecycle of HTLV-1 and other deltaretroviruses may be characterized by NC and MA functions that are distinct from those of the corresponding HIV-1 proteins, but together provide the functions required for viral replication.

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Section: Rna-binding Specificity Of Htlv-1 Gag Domainssupporting

confidence: 83%

mentioning

confidence: 99%

Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization

Hatterschide

Syu

et al. 2018

Journal of Biological Chemistry

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“…Studies of other retroviruses have suggested that the MA sequence contains functions in addition to membrane binding. These proposed roles include the packaging of Env glycoproteins and viral RNA during particle assembly and the targeting of integration complexes to the nucleus after the virus enters a host cell (5,20,57). Are these or any other functions provided by the RSV matrix sequence?…”

Section: Discussionmentioning

confidence: 99%

A large region within the Rous sarcoma virus matrix protein is dispensable for budding and infectivity

Nelle

Wills

1996

J Virol

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All retroviruses have a layer of matrix protein (MA) situated directly beneath the lipid of their envelope. This protein is initially expressed as the amino-terminal sequence of the Gag polyprotein, where it plays an important role in binding Gag to the plasma membrane during the early steps of the budding process. Others have suggested that MA may provide additional functions during virion assembly, including the selective incorporation of viral glycoproteins and the RNA genome into the emerging virion. To further study the role of the Rous sarcoma virus MA sequence in the viral replication cycle, we have pursued an extensive deletion analysis. Surprisingly, the entire second half of MA (residues 87 to 155) and part of the neighboring p2 sequence were found to be dispensable not only for budding but also for infectivity in avian cells. Thus, all of the functions associated with the Rous sarcoma virus MA sequence must be contained within its first half.

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“…Молекули, які утворилися, проникають в ядро клітини, де певна кількість лінійних молекул перетворюється в кільцеві і інтегрують в клітинний геном. Інтегрована лінійна ДНК-копіяретровірусного генома (провірус) передається дочірнім клітинам як складова частина генетичного матеріалу материнської клітини (Gulyukin et al, 1990;Katoh et al, 1991).…”

Section: вступunclassified

Вміст Ліпідів У Сироватці Крові Корів За Спонтанного Інфікування Вірусом Лейкозу Великої Рогатої Худоби

2016

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Lipids take part in the biological cycle of retroviruses and regulation of their expression. In particular, in the processes associated with the interaction with the lipid bilayer of the host cell (virus penetration into the cell), and budding of newly synthesized viral particles. To study the effect bovine leukemia virus on lipid metabolism in the host organism is very important for veterinary medicine, because of its effect on animal blood system. At the same time, changes in the blood system of lactating cows have a significant impact on the biochemical indicators of quantitative composition of the milk and therefore its quality, nutritional value and safety for consumers. Investigated the lipid composition of blood serum of cattle spontaneous infected with the virus leukemia. For study was formed by two groups of cows black–and–white breed in 3 years of age on 3 – 4 months of lactation, body weight were 400 – 450 kg, 6 animals in each. In the first (control) group was clinically healthy animals, free from bovine leukemia virus (according to the RID, ELISA and PCR studies), the second (experimental) was animals which infected by virus bovine leukemia. Established that in the infected animals significantly increases the content of total phospholipid by 6.0%, total and esterified cholesterol by 4.3 and 4.2%, respectively. Thus, the correlation of total cholesterol to total phospholipids was unchanged in both groups of animals. Also, in the blood serum of cows research groups noted increase of free fatty acids content by 83.3%. Thus, at the spontaneous infection the bovine leukemia virus in animals is a disturbance qualitative and quantitative composition of blood serum lipids owing to disturbance of synthetic processes in liver, caused by the necessity in the plastic material for build lipid layer of shell pathogen.

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Bovine leukemia virus matrix-associated protein MA(p15): further processing and formation of a specific complex with the dimer of the 5'-terminal genomic RNA fragment

Cited by 57 publications

References 42 publications

Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization

Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization

A large region within the Rous sarcoma virus matrix protein is dispensable for budding and infectivity

Вміст Ліпідів У Сироватці Крові Корів За Спонтанного Інфікування Вірусом Лейкозу Великої Рогатої Худоби

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