INTRODUCTIONMarginal regions cover relatively a large part of the Slovakia territory where the rearing of ruminants can efficiently produce animal commodities only occasionally. The geographic, social and economic stability of these regions are strongly influenced by the keeping of ruminants, particularly for marking milk production. Mastitis is considered to be the most frequent and most costly production disease in dairy herds. Mastitis is characterized by an inflammation in one or more quarters of the udder and can be either clinical or subclinical. The decrease in milk production per cow due to the clinical and subclinical prevalence of mastitis is usually recognised as the main cause of economic losses due to the disease [4,5,6].The factors influencing the susceptibility of the mammary gland to infections are: the presence of bacteria at the teat end, level of efficacy of the protective characteristics of the teat canal, and defence mechanisms in the udder (Fig. 1) The aim of this study was to evaluate, during early lactation, the prevalence and aetiological agents in two herds of dairy cows situated in the marginal parts of Slovakia.
MATERIALS AND METHODS
Animals and milkingThe study was conducted in accordance with good veterinary practice. The practical part of the study was carried out on two farms situated in marginal parts of Slovakia (Orava and Zemplín). On the first farm (A) we investigated 150 Slovak spotted breed cows and on the second (B) 380 dairy cows of the Holstein breed, between second and fourth lactations. The dairy cows on both farms were kept in a free housing system with individual boxes with bedding and ad libitum access to water and a separate calving barn. The keeping of cows corresponded to standard animal hygiene rules. Both herds were fed a total mixed rations based on grass silage, maize silage and concentrates.The cows from both herds were milked twice daily, herd A in a tandem parlour DeLaval 2x5 (Tumba, Sweden) and herd B in a parallel parlour Boumatic 2x12 Xpressway (Wisconsin, USA). The average milk yield was 6800 l and 7400 l per year in herds A and B, respectively. Blanket dry cow therapy (treatment of all quarters of all cows) was implemented in both herds.
Statistical analysisThe statistical analysis was performed using Graph-Pad PRISM 6.0 (GraphPad Software Inc., USA). The differences in incidence of mastitis among the herds were statistically analysed using the Chi-square test. The level of significance was set at P < 0.05.
RESULTSThe results obtained by the California mastitis test (CMT) are presented in Table 1. The elevation of SCC to the level below 200 000 ml -1 was detected in 63.2 % quarters on average. The average score (>1) of CMT above 400 000 ml Table 2).The microorganisms isolated from mastitis in the herds are presented in In our study the CoNS belong among the organisms most commonly isolated from milk samples of dairy cows with subclinical mastitis as in other studies [1,8,9].In the Netherlands, the prevalence of CoNS among bac-
CONCLUSIONSTh...