The objective of this study was to evaluate the effect of variations in milk protein composition on milk clotting properties and cheese yield. Milk was collected from 134 dairy cows of Swedish Red and White, Swedish Holstein, and Danish Holstein-Friesian breed at 3 sampling occasions. Concentrations of alphaS1-, beta-, and kappa-casein (CN), alpha-lactalbumin, and beta-lactoglobulin (LG) A and B were determined by reversed phase liquid chromatography. Cows of Swedish breeds were genotyped for genetic variants of beta- and kappa-CN. Model cheeses were produced from individual skimmed milk samples and the milk clotting properties were evaluated. More than 30% of the samples were poorly coagulating or noncoagulating, resulting in weak or no coagulum, respectively. Poorly and noncoagulating samples were associated with a low concentration of kappa-CN and a low proportion of kappa-CN in relation to total CN analyzed. Furthermore, the kappa-CN concentration was higher in milk from cows with the AB genotype than the AA genotype of kappa-CN. The concentrations of alphaS1-, beta-, and kappa-CN and of beta-LG B were found to be significant for the cheese yield, expressed as grams of cheese per one hundred grams of milk. The ratio of CN to total protein analyzed and the beta-LG B concentration positively affected cheese yield, expressed as grams of dry cheese solids per one hundred grams of milk protein, whereas beta-LG A had a negative effect. Cheese-making properties could be improved by selecting milk with high concentrations of alphaS1-, beta-, and kappa-CN, with high kappa-CN in relation to total CN and milk that contains beta-LG B.
Coal, fly ash, slag, and combustion gases from a large cyclone-fed power plant 870 MW(e) were analyzed for a suite of elements. Mass balance calculations show that the sampling and analyses were generally adequate to describe the flows of these elements through the plant. Most Hg, some Se, and probably most Cl and Br were discharged to the atmosphere as gases.
The degradation of chlorobenzene and its oxidation products by hydroxyl radicals generated with Fenton's reagent was studied. In the absence of oxygen, chlorophenols, dichlorobiphenyls (DCBs), and phenolic polymers were the predominant initial products. In the presence of oxygen, DCB yields decreased markedly and chlorobenzoquinone was also formed. Chlorophenol isomers were further oxidized by OH's to form chlorinated and nonchlorinated diols. DCBs and the phenolic polymers were also oxidized. The highest yield of product formed per mole of H202 consumed was observed in the pH range of 2-3. The pH dependence and product distributions suggest that complexes of aromatic intermediate compounds with iron and oxygen may play a role in regulating reaction pathways. At pH 3.0, approximately 5 mol of H202/mol of chlorobenzene were required to remove all of the aromatic intermediate compounds from solution.
This paper compares a number of one-dimensional closure models for the planetary boundary layer (PBL) that are currently in use in large-scale atmospheric models. Using the results of a large-eddy simulation (LES) model as the standardof comparison, the PBL models are evaluatedover a range of stratifications from free convective to neutral and a range of surface shear stresses. Capping inversion strengths for the convective cases range from weakly to strongly capped. Six prototypical PBL models are evaluated in this study, which focuses on the accuracy of the boundary-layer fluxes of momentum, heat, and two passive scalars. One scalar mimics humidity and the other is a top-down scalar entrained into the boundary layer from above. A set of measures based on the layer-averaged differences of these fluxes from the LES solutions is developed. In addition to the methodological framework and suite of LES solutions, the main result of the evaluation is the recognition that all of the examined PBL parameterizations have difficulty reproducing the entrainment at the top of the PBL, as given by the LES, in most parameter regimes. Some of the PBL models are relatively accurate in their entrainment flux in a subset of parameter regimes. The sensitivity of the PBL models to vertical resolution is explored, and substantive differences are observed in the performance of the PBL models, relative to LES, at low resolution typical of large scale atmospheric models.
Substantial variation in milk coagulation properties has been observed among dairy cows. Consequently, raw milk from individual cows and breeds exhibits distinct coagulation capacities that potentially affect the technological properties and milk processing into cheese. This variation is largely influenced by protein composition, which is in turn affected by underlying genetic polymorphisms in the major milk proteins. In this study, we conducted a large screening on 3 major Scandinavian breeds to resolve the variation in milk coagulation traits and the frequency of milk with impaired coagulation properties (noncoagulation). In total, individual coagulation properties were measured on morning milk collected from 1,299 Danish Holstein (DH), Danish Jersey (DJ), and Swedish Red (SR) cows. The 3 breeds demonstrated notable interbreed differences in coagulation properties, with DJ cows exhibiting superior coagulation compared with the other 2 breeds. In addition, milk samples from 2% of DH and 16% of SR cows were classified as noncoagulating. Furthermore, the cows were genotyped for major genetic variants in the αS1- (CSN1S1), β- (CSN2), and κ-casein (CSN3) genes, revealing distinct differences in variant frequencies among breeds. Allele I of CSN2, which had not formerly been screened in such a high number of cows in these Scandinavian breeds, showed a frequency around 7% in DH and DJ, but was not detected in SR. Genetic polymorphisms were significantly associated with curd firming rate and rennet coagulation time. Thus, CSN1S1 C, CSN2 B, and CSN3 B positively affected milk coagulation, whereas CSN2 A(2), in particular, had a negative effect. In addition to the influence of individual casein genes, the effects of CSN1S1-CSN2-CSN3 composite genotypes were also examined, and revealed strong associations in all breeds, which more or less reflected the single gene results. Overall, milk coagulation is under the influence of additive genetic variation. Optimal milk for future cheese production can be ensured by monitoring the frequency of unfavorable variants and thus preventing an increase in the number of cows producing milk with impaired coagulation. Selective breeding for variants associated with superior milk coagulation can potentially increase raw milk quality and cheese yield in all 3 Scandinavian breeds.
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