Bovine Herpesvirus 2 Infections

Scott, Frank

doi:10.1007/978-1-4613-1587-2_2

Cited by 4 publications

(3 citation statements)

References 45 publications

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“…In areas where the disease rarely occurs, it may be confused with other viral infections of the bovine teat. 18 Related herpesviruses such as BHV1 and BHV5 may cross-react in neutralization assays and induce similar cytopathic effects when isolated in cell culture. 12 Although BHV4 is not widely considered a major pathogen of the cattle industry, the ability to differentiate between BHV1 and BHV4 is important because of the difference in the relevance of these 2 viruses.…”

Section: Development Of a Polymerase Chain Reaction And Restriction Tmentioning

confidence: 99%

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Development of a Polymerase Chain Reaction and Restriction Typing Assay for the Diagnosis of Bovine Herpesvirus 1, Bovine Herpesvirus 2, and Bovine Herpesvirus 4 Infections

et al. 2002

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A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.

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Section: Development Of a Polymerase Chain Reaction And Restriction Tmentioning

confidence: 99%

“…Sensitivity of the PCR performed with 5 l of DNA extracted from 10-fold dilution series of BHV1 (KK/73, lanes 2-7), BHV2 (69/1LO, lanes 9-14), BHV4 (M-81, lanes[15][16][17][18][19][20]. Lanes 1 and 8 ϭ molecular weight marker, 100-bp ladder g .…”

mentioning

confidence: 99%

Development of a Polymerase Chain Reaction and Restriction Typing Assay for the Diagnosis of Bovine Herpesvirus 1, Bovine Herpesvirus 2, and Bovine Herpesvirus 4 Infections

et al. 2002

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“…The virus itself is naturally temperature sensitive and is isolated at temperatures as low as 32°C (13,16). No commercial vaccine exists for the virus and the only protection shown to be effective is vaccination by intramuscular injection of a virulent virus strain (15), but this type of vaccination can spread the infection as BHV-2 has the potential to become latent (16). Inactivated BHV-2 has been used to vaccinate both cattle against mammillitis (15) and even humans against genital herpes (herpes simplex virus type 2) (20).…”

mentioning

confidence: 99%