Bovine Collagen Implant: Histologic Chronology in Pig Dermis

Burke, Karen E.; Naughton, Gail K.; Waldo, Elaine; Cassai, Nick

doi:10.1111/j.1524-4725.1983.tb01036.x

Cited by 48 publications

(27 citation statements)

References 8 publications

(11 reference statements)

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“…This precise effect has been recently demonstrated by experiments in which intentionally differentially loaded IVDs (half of the disc in compression and half in tension) in a disc degeneration model (Lotz et al 2008). There are numerous other phenomena where a mechanochemical relationship between collagen and MMP may have relevance, including the instability of unloaded collagen implants (Burke et al 1983(Burke et al , 1985Donald 1986) and the documented loss of bone and ligament strength in microgravity (Vailas et al 1990;Zerath 1998). It is also interesting to consider exactly why fibroblasts, upon entering a randomly self-assembled collagen gel, contract the matrix (Bell et al 1979).…”

Section: Phil Trans R Soc a (2009)mentioning

confidence: 89%

Mechanical strain enhances survivability of collagen micronetworks in the presence of collagenase: implications for load-bearing matrix growth and stability

Bhole

Flynn

Liles

et al. 2009

Phil. Trans. R. Soc. A.

113

109

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There has been great interest in understanding the methods by which collagen-based load-bearing tissue is constructed, grown and maintained in vertebrate animals. To date, the responsibility for this process has largely been placed with mesenchymal fibroblastic cells that are thought to fully control the morphology of load-bearing extracellular matrix (ECM). However, given clear limitations in the ability of fibroblastic cells to precisely place or remove single collagen molecules to sculpt tissue, we have hypothesized that the material itself must play a critical role in the determination of the form of structural ECM. We here demonstrate directly, using live, dynamic, differential interference contrast imaging, that mechanically strained networks of collagen fibrils, exposed to collagenase (Clostridium histolyticum), degrade preferentially. Specifically, unstrained fibrils are removed 'quickly', while strained fibrils persist significantly longer. The demonstration supports the idea that collagen networks are mechanosensitive in that they are stabilized by mechanical strain. Thus, collagen molecules (together with their complement enzymes) may comprise the basis of a smart, load-adaptive, structural material system. This concept has the potential to drastically simplify the assumed role of the fibroblast, which would need only to provide ECM molecules and mechanical force to sculpt collagenous tissue.

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Section: Phil Trans R Soc a (2009)mentioning

confidence: 89%

Mechanical strain enhances survivability of collagen micronetworks in the presence of collagenase: implications for load-bearing matrix growth and stability

Bhole

Flynn

Liles

et al. 2009

Phil. Trans. R. Soc. A.

113

109

View full text Add to dashboard Cite

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“…Antibovine collagen and antibovine serum titers do not increase in recipients, 17,19 nor is evidence of immediate or delayed immune reactivity to xenogenic collagen observed. 18 Although in some instances the implanted gels have been shown to degrade rapidly 20 and the space previously occupied by the gels may not necessarily be replaced by neodermis, 21 successful neodermis formation has been reported using collagen-glycosaminoglycan gels. 20,[22][23][24] Alternatively, deepidermized xenograft porcine or human skin has been found to have modest value in treating fullthickness skin wounds in conjunction with immediate STSG autografts.…”

Section: Resultsmentioning

confidence: 99%

“…18 Although in some instances the implanted gels have been shown to degrade rapidly 20 and the space previously occupied by the gels may not necessarily be replaced by neodermis, 21 successful neodermis formation has been reported using collagen-glycosaminoglycan gels. 20,[22][23][24] Alternatively, deepidermized xenograft porcine or human skin has been found to have modest value in treating fullthickness skin wounds in conjunction with immediate STSG autografts. 25,26 Such matrices may exhibit residual antigenicity as a result of the incomplete removal of cellular debris from the dermis or processing-dependent alterations in the connective tissue matrix.…”

Section: Resultsmentioning

confidence: 99%

Use of Porcine Acellular Dermal Matrix as a Dermal Substitute in Rats

Srivastava¹,

DeSagun²,

Jennings³

et al. 2001

Annals of Surgery

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ObjectiveTo examine porcine acellular dermal matrix (ADM) as a xenogenic dermal substitute in a rat model. Summary Background DataAcellular dermal matrix has been used in the treatment of fullthickness skin injuries as an allogenic dermal substitute providing a stable wound base in human and animal studies. MethodsXenogenic and allogenic ADMs were produced by treating porcine or rat skin with Dispase and Triton X-100. Full-thickness skin defects (225 mm 2 ) were created on the dorsum of rats (n ϭ 29), porcine or rat ADMs were implanted in them, and these were overlain with ultrathin split-thickness skin grafts (STSGs). In two adjacent wounds, 0.005-or 0.017-inch-thick autografts were implanted. In other experiments, the antimicrobial agent used during ADM processing (azide or a mixture of antibiotics) and the orientation of the implanted ADM (papillary or reticular side of ADM facing the STSG) were studied. Grafts were evaluated grossly and histologically for 30 days after surgery. ResultsSignificant wound contraction was seen at 14, 20, and 30 days after surgery in wounds receiving xenogenic ADM, allogenic ADM, and thin STSGs. Contraction of wounds containing xenogenic ADM was significantly greater than that of wounds containing allogenic ADM at 30 days after surgery. Graft take was poor in wounds containing xenogenic ADM and moderately good in those containing allogenic ADM. Wound healing was not significantly affected by the antimicrobial agent used during ADM preparation or by the ADM orientation. ConclusionDispase-Triton-treated allogenic ADM was useful as a dermal substitute in full-thickness skin defects, but healing with xenogenic ADM was poor.In extensive deep burns and other full-thickness skin wounds, permanent replacement of lost skin remains a major challenge. Several methods of wound closure are in use, and each has its own advantages and disadvantages. Porcine skin and preserved cadaver skin are used for temporary wound coverage, but 1 to 2 weeks after grafting, these tissues undergo immune-mediated rejection.1,2 Permanent wound coverage is usually accomplished using meshed, split-thickness autografts harvested from undamaged regions of skin. Extensively burned patients have limited donor sites, so thin split-thickness autografts are harvested repeatedly from the same sites. This results in substantial donor-site problems resulting from pain, infection, scarring, and sometimes keloid formation.3 Very thin (0.005-inchthick) meshed autografts can be used, but the lack of a sufficient dermal bed often results in extensive wound contraction at the recipient site. 4 The harvest of thicker splitthickness skin grafts (STSGs) reduces this contraction problem but causes increased problems at donor sites. Alternatively, cultured epidermal autografts expanded using cell culture methods can be used for wound coverage, but this technique is expensive, and cultured epidermal autografts often fail to survive or result in poor-quality wound healing.5-8 Many of these difficulties with STSGs and cul-

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“…Collagen was first employed as a biomaterial in medical surgery in the late 19th century (Burke et al, 1983;Silver et al, 1997). Subsequently, it was used in many other medical applications, e.g.…”

Section: Collagen Vs Gelatine As Biomaterialsmentioning

confidence: 99%