Bovine C-terminal octapeptide of RFamide-related peptide-3 suppresses luteinizing hormone (LH) secretion from the pituitary as well as pulsatile LH secretion in bovines

Kadokawa, Hiroya; Shibata, Masahiro; Tanaka, Yoshinori; Kojima, Takatoshi; Matsumoto, Kazunori; Oshima, Kazunaga; Yamamoto, Naoyuki

doi:10.1016/j.domaniend.2009.02.001

Cited by 126 publications

(103 citation statements)

References 22 publications

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“…5-7). Mammalian GnIH orthologs have also been identified in the brains of various mammalian species (8 -13), and the inhibitory effect of GnIH orthologs on gonadotropin release has been demonstrated (13)(14)(15)(16)(17)(18)(19). GnIH and its orthologs also inhibit gonadotropin common ␣-subunit and specific ␤-subunit transcription in the pituitary (20 -22).…”

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Gonadotropin-Inhibitory Hormone Inhibits GnRH-Induced Gonadotropin Subunit Gene Transcriptions by Inhibiting AC/cAMP/PKA-Dependent ERK Pathway in LβT2 Cells

et al. 2012

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A neuropeptide that directly inhibits gonadotropin secretion from the pituitary was discovered in quail and named gonadotropin-inhibitory hormone (GnIH). The presence and functional roles of GnIH orthologs, RF-amide-related peptides (RFRP), that possess a common C-terminal LPXRF-amide (X ϭ L or Q) motif have also been demonstrated in mammals. GnIH orthologs inhibit gonadotropin synthesis and release by acting on pituitary gonadotropes and GnRH neurons in the hypothalamus via its receptor (GnIH receptor). It is becoming increasingly clear that GnIH is an important hypothalamic neuropeptide controlling reproduction, but the detailed signaling pathway mediating the inhibitory effect of GnIH on target cells is still unknown. In the present study, we investigated the pathway of GnIH cell signaling and its possible interaction with GnRH signaling using a mouse gonadotrope cell line, L␤T2. First, we demonstrated the expression of GnIH receptor mRNA in L␤T2 cells by RT-PCR. We then examined the inhibitory effects of mouse GnIH orthologs [mouse RFRP (mRFRP)] on GnRH-induced cell signaling events. We showed that mRFRP effectively inhibited GnRH-induced cAMP signaling by using a cAMP-sensitive reporter system and measuring cAMP levels, indicating that mRFRP function as an inhibitor of adenylate cyclase. We further showed that mRFRP inhibited GnRH-stimulated ERK phosphorylation, and this effect was mediated by the inhibition of the protein kinase A pathway. Finally, we demonstrated that mRFRP inhibited GnRHstimulated gonadotropin subunit gene transcriptions and also LH release. Taken together, the results indicate that mRFRP function as GnIH to inhibit GnRH-induced gonadotropin subunit gene transcriptions by inhibiting adenylate cyclase/cAMP/protein kinase A-dependent ERK activation in L␤T2 cells. (Endocrinology 153: 2332-2343, 2012)

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Gonadotropin-Inhibitory Hormone Inhibits GnRH-Induced Gonadotropin Subunit Gene Transcriptions by Inhibiting AC/cAMP/PKA-Dependent ERK Pathway in LβT2 Cells

et al. 2012

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“…Isso indica que essa região possui um papel chave na interação dos esteróides, sinalizadores metabólicos e ambientais com os neurônios secretores de GnRH. As ações indiretas dos esteróides em ratos são mediadas por neurotransmissores, principalmente o ácido gama aminobutírico (GABA) e o glutamato (JARRY et al, 1992). Aparentemente, todos os neurônios do GABA localizados no AVPV expressam ERs (PETERSEN et al, 2003).…”

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“…Além disso, o estradiol 17ß induz a expressão dos receptores de progesterona nos neurônios GABA do AVPV (SHUGHRUE et al, 1997;PETERSEN et al, 2003), o que parece ser indispensável, ao menos em roedores, para que o estradiol induza o pico pré-ovulatório do LH (CHAPPELL & LEVINE, 2000). A liberação do glutamato também é regulada por estradiol (JARRY et al, 1992) e a ativação dos seus receptores estimula a expressão de GnRH e liberação de LH (DHANDAPANI & BRANN, 2000). O estradiol atua no controle do GnRH não só alterando as respostas sinápticas via mecanismos genômicos (regulação na expressão de receptores e RNAm), mas também modulando a comunicação entre células de maneira bastante aguda.…”

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Controle sobre GnRH durante o anestro pós-parto em bovinos

et al. 2010

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“…Bentley et al (2008) reported the expression of GnIH and GnIH receptor doi: 10.17221/58/2016-CJAS (GnIH-R) in the avian reproductive system, including gonads and accessory reproductive organs. A mammalian GnIH ortholog (RFRP-3) inhibits LH release in rats (Murakami et al 2008) and cattle (Kadokawa et al 2009). Clarke et al (2008) also found that peripheral administration of the deduced ovine GnIH homolog, RFRP-3, reduced the amplitude of LH pulses in sheep.…”

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Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size

An¹,

Bao²,

Hou³

et al. 2017

CZECH J ANIM SCI

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An X., Bao L., Hou J., Bai Y., Zhao X., Song Y., Cao B. . (2017): Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size. Czech J. Anim. Sci., 62, 269-275.Gonadotropin-inhibitory hormone (GnIH) can decrease luteinizing hormone and/or follicle-stimulating hormone levels in rat, mouse, sheep, and cattle by the direct suppression of gonadotropin-releasing hormone (GnRH). The present study investigated polymorphisms in the GnIH genes of two dairy goat breeds and their association with litter size. Single nucleotide polymorphisms (SNPs) g.1837C>G and g.3195G>A (GenBank Accession Nos. KR778885 and KR819142) were detected in the GnIH genes of Xinong Saanen and Guanzhong dairy goat breeds using DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Furthermore, the g.1837C>G and g.3195G>A loci were closely linked in both breeds (r 2 > 0.33). Association analysis showed that these SNPs had significant effects on the litter size of goats (P < 0.05). In both breeds, individuals with the CC/GG (g.1837C>G/g.3195G>A) genotype showed larger litter sizes in the second and average parities than individuals with the GG/AA genotype (P < 0.05). Known biochemical and physiological functions, along with our results, indicate that the CC/GG genotype may be used in marker-assisted selection to choose individuals with a larger litter size from both breeds.

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Bovine C-terminal octapeptide of RFamide-related peptide-3 suppresses luteinizing hormone (LH) secretion from the pituitary as well as pulsatile LH secretion in bovines

Cited by 126 publications

References 22 publications

Gonadotropin-Inhibitory Hormone Inhibits GnRH-Induced Gonadotropin Subunit Gene Transcriptions by Inhibiting AC/cAMP/PKA-Dependent ERK Pathway in LβT2 Cells

Gonadotropin-Inhibitory Hormone Inhibits GnRH-Induced Gonadotropin Subunit Gene Transcriptions by Inhibiting AC/cAMP/PKA-Dependent ERK Pathway in LβT2 Cells

Controle sobre GnRH durante o anestro pós-parto em bovinos

Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size

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