Bovine bta-microRNA-1271 Promotes Preadipocyte Differentiation by Targeting Activation Transcription Factor 3

Xu, Hongyan; Shao, Jiahao; Yin, Baozhen; Zhang, L M; Fang, Jiachen; Zhang, J S; Xia, Guangjun

doi:10.1134/s0006297920070032

Cited by 10 publications

(7 citation statements)

References 38 publications

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“…miR‐1271 has been shown to indirectly inhibit the Wnt/β‐catenin pathway and TGFβ signaling by targeting FOXQ1 and CAPN4 , respectively (Xiang et al ., 2015; Li et al ., 2018). Plus, bta‐miR‐1271 has been shown to directly bind on ATF3 gene and promoted preadipocyte differentiation in Yanbian cattle (Xu et al ., 2020). So, bta‐miR‐1271 may be one key to explain the fat deposition mechanism.…”

Section: Discussionmentioning

confidence: 99%

Integrated microRNA–mRNA analysis reveals the roles of microRNAs in the muscle fat metabolism of Yanbian cattle

Zhang

Fang

et al. 2021

Animal Genetics

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Summary Fat deposition is an important economic trait in farm animals. However, it is difficult to genetically improve intramuscular fat deposition via trait‐based cattle breeding. The main objectives of this study were to analyze the factors about beef flavor, and to detect functional microRNA (miRNA, miR) associated with intramuscular fat deposition in Yanbian cattle. Longissimus dorsi samples from six steers were separated into high‐ and low‐fat groups (n = 3 each) based on the marbling score, and transcriptomic analysis was performed using miRNA sequencing. A total of 33 miRNAs and 38 genes were found to be differentially expressed in the high‐ and low‐fat groups. Quantitative real‐time polymerase chain reaction was performed to validate the sequencing results. Integrated miRNA–mRNA analysis revealed that miRNA‐associated target genes were primarily associated with skeletal muscle development. However, some of the miRNAs (miR‐424 etc.) and genes (ATF3 etc.) were also associated with fat metabolism. A targeted relationship between miR‐22‐3p and the WFIKKN2 gene and its involvement in adipocyte differentiation were confirmed experimentally. The study findings may provide potential candidate molecular targets for the selection of cattle with improved meat quality.

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Section: Discussionmentioning

confidence: 99%

Integrated microRNA–mRNA analysis reveals the roles of microRNAs in the muscle fat metabolism of Yanbian cattle

Zhang

Fang

et al. 2021

Animal Genetics

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“…Previous studies have identified that miRNAs directly act on the 3 UTR of their target genes to regulate adipocyte proliferation and differentiation [12,33,34]. For instance, bta-miR-1271 promotes differentiation of bovine preadipocytes via targeting the activating transcription factor 3 (ATF3) [35], and bta-miR-224 suppresses lipogenic differentiation of bovine preadipocytes by targeting LPL [36]. SUMO1 was predicted as a target gene of bta-miR-2400, and previous studies revealed that SUMO1 was associated with adipogenesis [37].…”

Section: Discussionmentioning

confidence: 99%

Bta-miR-2400 Targets SUMO1 to Affect Yak Preadipocytes Proliferation and Differentiation

Zhang

et al. 2021

Biology

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Yak adipose tissue may have evolved a unique energy metabolism manner to accommodate the organism’s seasonal growth rhythms. MiRNAs regulate multiple biological processes including systemic metabolism and energy homeostasis through post-transcriptional regulations. Rare reports have shown that miRNAs regulate lipid metabolism in domestic yaks. Therefore, we investigated the regulatory mechanisms of bta-miR-2400 in modulating yak preadipocytes proliferation and differentiation. We found that bta-miR-2400 was highly expressed in adipose tissue. Overexpression of bta-miR-2400 in yak preadipocytes significantly enhanced cell proliferation, increased the number of EdU fluorescence-stained cells, and promoted the expression of proliferation marker genes (CDK2, CDK4 and PCNA). Besides, overexpression of bta-miR-2400 repressed the expression of adipogenesis-related marker genes, and the content of cellular triglyceride was substantially reduced. Conversely, inhibition of bta-miR-2400 showed opposite effects compared to those of bta-miR-2400 overexpression in yak preadipocytes. Further, luciferase reporter assays revealed that SUMO1 is a target gene of bta-miR-2400, with bta-miR-2400 being able to down-regulate SUMO1 mRNA and protein expression. In conclusion, bta-miR-2400 regulates lipid metabolism and energy homeostasis in yak preadipocytes by directly targeting SUMO1 to promote cell proliferation and inhibit differentiation.

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“…miRNAs are important regulators of fat formation and lipid metabolism. Xu et al [19] cultured Yanbian yellow cattle precursor adipocytes in vitro and transfected miRNA and found that miR-1271 could inhibit the expression of the ATF3 gene and promote the adipogenic differentiation of precursor adipocytes. In addition, miR-381 can also inhibit the expression of the KCTD15 gene [20].…”

Section: Discussionmentioning

confidence: 99%

miR-15a Targets ABAT Gene to Regulate the Differentiation of Preadipocytes of Yanbian Yellow Cattle

Bai

Fang

et al. 2021

Preprint

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BackgroundMicroRNAs (miRNAs) are small RNAs that cannot be translated into proteins. The length of a mature miRNA is approximately 20-25 nt. Neural regulation, inflammatory response, cell proliferation and apoptosis, fat metabolism, and cell differentiation, among others, have become research hotspots in recent years. In this experiment, dual-luciferase reporter gene detection, qRT-PCR, oil red O staining, western blotting, and other methods were used to select the differentially expressed miR-15a and its target gene, the 4-aminobutyrate aminotransferase (ABAT) gene, in the longissimus muscle tissues of cattle bulls and steers. By transfecting Yanbian yellow cattle precursor adipocytes with equal amounts of artificially synthesised miR-15a mimics, mimics NC, miR-15a inhibitor, and inhibitor NC and inducing differentiation, the total RNA and total protein of cells during the induced differentiation process were extracted.The effect of miR-15a on the mRNA and protein expression of adipogenesis-related genes was analysed. ResultsThe results showed that the lipid droplet content and lipid droplet size of the miR-15a mimic group were significantly lower than those of the mimics NC group, and the mRNA levels of the peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT enhancer-binding protein α (C/EBPα) genes in the miR-15a mimics group were significantly lower than those in the mimics NC group. At the same time, the protein expression of miR-15a mimic group cells was also significantly lower than that of the NC group cells. Meanwhile, the mRNA levels of the PPARγ and C/EBPα genes of miR-15a inhibitor group cells were significantly higher than those of the NC group cells, and the protein expression levels were also significantly higher than those of the NC group cells. The luciferase reporter results showed that miR-15a mimics significantly inhibited the activity of the ABAT-3'UTR dual luciferase reporter gene vector, indicating that the miR-15a and ABAT genes have a targeting effect, and that overexpressed miR-15a significantly reduced the expression of the ABAT gene in the cell, inhibited the expression of miR-15a, and significantly increased the expression of the ABAT.ConclusionsIn summary, miR-15a may target the ABAT gene to regulate the differentiation of Yanbian yellow cattle precursor adipocytes.

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Bovine bta-microRNA-1271 Promotes Preadipocyte Differentiation by Targeting Activation Transcription Factor 3

Cited by 10 publications

References 38 publications

Integrated microRNA–mRNA analysis reveals the roles of microRNAs in the muscle fat metabolism of Yanbian cattle

Integrated microRNA–mRNA analysis reveals the roles of microRNAs in the muscle fat metabolism of Yanbian cattle

Bta-miR-2400 Targets SUMO1 to Affect Yak Preadipocytes Proliferation and Differentiation

miR-15a Targets ABAT Gene to Regulate the Differentiation of Preadipocytes of Yanbian Yellow Cattle

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