Bta-miR-2400 Targets SUMO1 to Affect Yak Preadipocytes Proliferation and Differentiation

Abstract

Yak adipose tissue may have evolved a unique energy metabolism manner to accommodate the organism’s seasonal growth rhythms. MiRNAs regulate multiple biological processes including systemic metabolism and energy homeostasis through post-transcriptional regulations. Rare reports have shown that miRNAs regulate lipid metabolism in domestic yaks. Therefore, we investigated the regulatory mechanisms of bta-miR-2400 in modulating yak preadipocytes proliferation and differentiation. We found that bta-miR-2400 was highly expressed in adipose tissue. Overexpression of bta-miR-2400 in yak preadipocytes significantly enhanced cell proliferation, increased the number of EdU fluorescence-stained cells, and promoted the expression of proliferation marker genes (CDK2, CDK4 and PCNA). Besides, overexpression of bta-miR-2400 repressed the expression of adipogenesis-related marker genes, and the content of cellular triglyceride was substantially reduced. Conversely, inhibition of bta-miR-2400 showed opposite effects compared to those of bta-miR-2400 overexpression in yak preadipocytes. Further, luciferase reporter assays revealed that SUMO1 is a target gene of bta-miR-2400, with bta-miR-2400 being able to down-regulate SUMO1 mRNA and protein expression. In conclusion, bta-miR-2400 regulates lipid metabolism and energy homeostasis in yak preadipocytes by directly targeting SUMO1 to promote cell proliferation and inhibit differentiation.

Runs of Homozygosity Analysis Reveals Genomic Diversity and Population Structure of an Indigenous Cattle Breed in Southwest China

“…Other functional genes revealed in this study were also previously reported to be associated with diverse production traits, including the RCN2 (reticulocalbin 2), PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1), ADRB3 (adrenoceptor beta 3) genes with carcass traits in Hanwoo and Qinchuan cattle [ 35 , 36 ], GABRB3 (gamma-aminobutyric acid type A receptor subunit beta3) and GABRB5 (gamma-aminobutyric acid type A receptor subunit alpha5) genes with temperament in beef cattle [ 37 ], ZNF703 (zinc finger protein 703) and ERLIN2 (ER lipid raft associated 2) genes with average daily gain in Nellore and with residual concentrate intake in Holstein [ 38 ], and GOT1L1 (Glutamic-oxaloacetic transaminase 1 like 1) as a cell indicator of stress in the cattle semen [ 39 ]. Interestingly, we also mapped a microRNA gene of bta-mir-2400 that was experimentally revealed for affecting the preadipocytes proliferation and differentiation in yak [ 40 ], regulating skeletal muscle satellite cells proliferation in Chinese Simmental cattle [ 41 ]. On the whole, the current literature evidence confirms that the revealed significant genomic regions are likely associated with the local environmental adaptations and other production traits in Xieka cattle.…”

Bta-miR-484 Targets SFRP1 and Affects Preadipocytes Proliferation, Differentiation, and Apoptosis

“…Additionally, miR-26 [ 6 ] and miR-107 [ 7 ] inhibit adipocyte differentiation and adipogenesis by targeting F-box and leucine-rich repeat protein 19 ( FBXL19 ) and cyclin-dependent kinase 6 ( CDK6 ). Alternatively, bta-miR-150 [ 8 ] and bta-miR-2400 [ 9 ] have been determined to promote bovine adipocyte proliferation and inhibit differentiation. Bta-miR-149-5p [ 10 ] and bta-miR-376a [ 11 ] target CREB regulated transcription coactivator ( CRTC ) and KLF transcription factor 15 ( KLF15 ), which inhibits the proliferation and differentiation of bovine adipocytes.…”