Bovine Adenovirus

Abstract

Two adenovirus strains were isolated in calf testicle cell cultures from blood specimens of cattle in Japan. This is the first isolation of bovine adenovirus reported in Japan. The isolates were antigenically similar to each other and distinct from the hitherto described serotypes 1, 2 and 3 of bovine adenovirus. Unfortunately, bovine adenovirus types 4 and 5 were not available for comparison, and hence, until the matter is settled, the virus will be called "Bovine adenovirus type Nagano". Nagano virus was identified as adenovirus on the bases of the inhibitory effect of 5-iodo-2'-deoxyuridine on virus replication, ether-resistance, effect of temperature and pH on infectivity, and fine structure of the virus particle. The virus grew and formed intranuclear inclusion bodies, a characteristic of adenovirus, in bovine testicle cells but not in bovine kidney cells. The virus agglutinated rat erythrocytes very poorly, but not sheep, goat, cattle, horse, guinea pig, hamster, chicken, and mouse cells. The virus produced adenovirus group-specific antigen in cell cultures. Sero-negative calves were readily infected with the virus by the intravenous, subcutaneous, oral or intranasal routes of inoculation. The infected animals produced antibodies and showed a mild clinical reaction comprised of rhinorrhea, diarrhea and a degree of pyrexia; low-titered viremia of short duration and leukopenia were also observed. A serologic survey indicated wide-spread dissemination of the virus among Japanese cattle, but further studies are needed to determine the etiologic significance of the virus in the natural disease in cattle.

Bovine Epizootic Fever

“…Tests were carried out by the complement dilution method previously described [8,11]. The bovine serum to be tested, without heating, was used at 1:2 dilution in veronal-buffered saline at pH 7.2.…”

Serological Typing of Bovine Adenovirus, Nagano and Fukuroi, as Type 4 and New Type 6

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We reported the isolation of adenovirus strains of two distinct serotypes, Nagano and Fukuroi, in bovine testicle cell culture from Japanese cattle [3,6]. The strains of bovine adenovirus types 1, 2 and 3 were the same as used in the previous studies [6]. Recently we obtained the prototype strains of these types.

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“…The strains of bovine adenovirus types 1, 2 and 3 were the same as used in the previous studies [6]. The strains of bovine adenovirus types 1, 2 and 3 were the same as used in the previous studies [6].…”

“…We reported the isolation of adenovirus strains of two distinct serotypes, Nagano and Fukuroi, in bovine testicle cell culture from Japanese cattle [3,6]. These types were shown to be antigenically different from types 1 [4], 2 [5] and 3 [2] of bovine adenovirus, but types 4 and 5 [1] were not available for comparison.…”

Nomi Virus, a Virus Isolated from an Apparently New Epizootic Respiratory Disease of Cattle

“…The technique was described elsewhere [6]. A pooled sample of nasal secretions from seven affected cattle in a herd in Kanagawa Prefecture yielded a cytopathic agent, NMKP, in bovine kidney cell cultures.…”