Bottom-up biofilm eradication using bacteriophage-loaded magnetic nanocomposites: a computational and experimental study

Yu, Pingfeng; Wang, Zijian; Marcos-Hernández, Mariana; Zuo, Pengxiao; Zhang, Danning; Powell, Camilah D.; Pan, Aaron Y.; Villagrán, Dino; Wong, Michael S.; Alvarez, Pedro J. J.

doi:10.1039/c9en00827f

Cited by 19 publications

(27 citation statements)

References 45 publications

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“…Both small and large PNCs dispersed phages evenly throughout the biofilm bottom, significantly disrupting the biofilm bottom layer and detaching the biofilm within 6 hours, with efficient biofilm removal for dual and multi-species biofilm. 128 Phages with broad host range are also conjugated with magnetic colloidal nanoparticle clusters to facilitate biofilm penetration and subsequent removal in established biofilms. 129 With the continuous evolution of bacteria, such manipulation of phages with nanoparticles, may more than ever, be needed now in the fight against biofilm forming and/or their associated human infections due to antimicrobial resistant bacteria.…”

Section: Application Of Phages As Vehicles To Deliver Antibiofilm Agentsmentioning

confidence: 99%

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Amankwah¹,

Abdella²,

Kassa³

2021

NSA

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Biofilms are bacterial communities that live in association with biotic or abiotic surfaces and enclosed in an extracellular polymeric substance. Their formation on both biotic and abiotic surfaces, including human tissue and medical device surfaces, pose a major threat causing chronic infections. In addition, current antibiotics and antiseptic agents have shown limited ability to completely remove biofilms. In this review, the authors provide an overview on the formation of bacterial biofilms and its characteristics, burden and evolution with phages. Moreover, the most recent possible use of phages and phage-derived enzymes to combat bacteria in biofilm structures is elucidated. From the emerging results, it can be concluded that despite successful use of phages and phage-derived products in destroying biofilms, they are mostly not adequate to eradicate all bacterial cells. Nevertheless, a combined therapy with the use of phages and/or phage-derived products with other antimicrobial agents including antibiotics, nanoparticles, and antimicrobial peptides may be effective approaches to remove biofilms from medical device surfaces and to treat their associated infections in humans.

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Section: Application Of Phages As Vehicles To Deliver Antibiofilm Agentsmentioning

confidence: 99%

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Amankwah¹,

Abdella²,

Kassa³

2021

NSA

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“…This is due to the significant action of bacteriophages destroying the integrity of the bacterial biofilm through destruction of the cells producing the biofilm matrix, which has been confirmed in numerous experiments. 17 − 19 …”

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confidence: 99%

Therapeutic and Prophylactic Effect of the Experimental Bacteriophage Treatment to Control Diarrhea Caused by E. coli in Newborn Calves

et al. 2021

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The prevalence of antibiotic-resistant bacteria causing neonatal diarrhea in calves has become a serious problem in the control of infection. Due to increasing antibiotic resistance, bacteriophages with probiotics are considered the best alternative. The aim of the study was to evaluate the use of a suppository containing probiotic strains of Lactobacillus spp. and bacteriophages specific for pathogenic E. coli in young calves with diarrhea. The study evaluated therapeutic and prophylactic effects (specific and nonspecific humoral response). The study was carried out on 24 female HF calves, aged 2 to 7 days and weighing from 35 to 46 kg. The calves were divided into four groups ( n = 6) as follows: Group 1, healthy control that received no medicine; Group 2, positive control with diarrhea; Group 3, healthy calves that received medicine; Group 4, calves with diarrhea that received medicine. The animals received suppositories containing Lactobacillus spp. and bacteriophages specific for pathogenic E. coli for 5 days. On the first day, the calves received the suppositories twice—in the morning and 12 h later; subsequently they were administered once a day. The health status of the calves was observed for 11 days after the first application of suppositories. A protective and preventive effect of the experimental therapy was obtained in the research. The probiotic-phage suppositories reduced the duration of diarrhea in calves, completely eliminating it within 24–48 h after use. The therapy stimulated the activation of immune mechanisms in calves, which translated into an enhanced specific and nonspecific response and increased resistance to infection.

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“…Recent studies have identified that phages can be immobilized onto nanocomposites by physical adsorption, based on electrostatic adhesion, or chemical binding [94]. It has been observed that the conjugation of polyvalent Podoviridae phages and magnetic colloidal nanoparticle clusters removed 98.3 ± 1.4% of the dual-species biofilm and 92.2 ± 3.1% of the multispecies biofilm coverage area after 6 h of treatment [95], whereas this binding approach can extend the application of phages in microbial control by enhancing their direct delivery to the relatively inaccessible inner layer of biofilms with the help of low-energy magnetic fields [96]. Furthermore, a study revealed that nano-TiO 2 could promote phage gM13 attachment on the cell surface of E. coli TG1, which contributed to the infectious entry of phage gM13 [97].…”

Section: The Combination Of Phage With Other Strategiesmentioning

confidence: 99%

Phages against Pathogenic Bacterial Biofilms and Biofilm-Based Infections: A Review

Liu

Zhang

et al. 2022

Pharmaceutics

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Bacterial biofilms formed by pathogens are known to be hundreds of times more resistant to antimicrobial agents than planktonic cells, making it extremely difficult to cure biofilm-based infections despite the use of antibiotics, which poses a serious threat to human health. Therefore, there is an urgent need to develop promising alternative antimicrobial therapies to reduce the burden of drug-resistant bacterial infections caused by biofilms. As natural enemies of bacteria, bacteriophages (phages) have the advantages of high specificity, safety and non-toxicity, and possess great potential in the defense and removal of pathogenic bacterial biofilms, which are considered to be alternatives to treat bacterial diseases. This work mainly reviews the composition, structure and formation process of bacterial biofilms, briefly discusses the interaction between phages and biofilms, and summarizes several strategies based on phages and their derivatives against biofilms and drug-resistant bacterial infections caused by biofilms, serving the purpose of developing novel, safe and effective treatment methods against biofilm-based infections and promoting the application of phages in maintaining human health.

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Bottom-up biofilm eradication using bacteriophage-loaded magnetic nanocomposites: a computational and experimental study

Abstract: The combined experimental and computational study demonstrates an inverse relationship between phage-nanocomposite conjugate (PNC) size and biofilm eradication potential.

Cited by 19 publications

References 45 publications

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Therapeutic and Prophylactic Effect of the Experimental Bacteriophage Treatment to Control Diarrhea Caused by E. coli in Newborn Calves

Phages against Pathogenic Bacterial Biofilms and Biofilm-Based Infections: A Review

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