Botryosphaeria corticola, sp. nov. on Quercus Species, with Notes and Description of Botryosphaeria stevensii and Its Anamorph, Diplodia mutila

Alves, Artur; Correia, António; Luque, Jordi; Phillips, Alan J. L.

doi:10.2307/3762177

Cited by 116 publications

(104 citation statements)

References 20 publications

Supporting

Mentioning

101

Contrasting

Order By: Relevance

“…The internal transcribed spacer (ITS) region of the ribosomal DNA cluster and part of the translation elongation factor 1-alpha (tef1-a) were sequenced for the 32 selected isolates as described previously (Alves et al 2004(Alves et al , 2008, and the sequences deposited in GenBank ( Table 2). The amplified PCR fragments were purified with the DNA Clean and Concentratorä-5 kit (Zymo Research, California, USA).…”

Section: Molecular Identification Of Isolatesmentioning

confidence: 99%

See 1 more Smart Citation

Phylogeny and pathogenicity of Lasiodiplodia species associated with dieback of mango in Peru

et al. 2017

Self Cite

View full text Add to dashboard Cite

Section: Molecular Identification Of Isolatesmentioning

confidence: 99%

“…Genomic DNA was extracted from mycelium as described by Alves et al (2004). PCR reactions were carried out with NZYTaq 2 Â Green Master Mix (NZYTech, Lisboa, Portugal).…”

Section: Molecular Identification Of Isolatesmentioning

confidence: 99%

Phylogeny and pathogenicity of Lasiodiplodia species associated with dieback of mango in Peru

et al. 2017

Self Cite

View full text Add to dashboard Cite

“…A set of 19 isolates representing each MSP-PCR cluster was selected for a phylogenetic analysis of the ITS region following the protocol of Alves et al (2004) and increasing the amount of primers to 25 pmol each and of Taq polymerase to 1.25 U. The cycling conditions were: initial denaturation of 10 min at 95°C, 35 cycles of 1 min at 95°C, 1 min at 55°C and 30 sec at 72°C, and a final extension step of 10 min at 72°C.…”

Section: Isolationsmentioning

confidence: 99%

Phylogeny, morphology and pathogenicity of Diaporthe and Phomopsis species on almond in Portugal

2010

View full text Add to dashboard Cite

The ascomycete genus Diaporthe includes plant pathogens and endophytes on a wide range of hosts including economically important crops. Anamorphs are coelomycetous and reside in the genus Phomopsis. Phomopsis amygdali is the causal agent of twig canker and blight of almonds. In a recent survey of dieback of almonds in Portugal, the most frequent fungi detected were Diaporthe/Phomopsis species. Isolates from almond and other Prunus species were characterised and grouped according to their microsatelliteprimed PCR (MSP-PCR) profiles and representatives of the different groups were selected for a phylogenetic study based on the ITS rDNA region (ITS1-5.8S-ITS2). Combining morphological, cultural, molecular and pathogenicity data, three species were distinguished. Phomopsis amygdali was shown to be the main pathogen on almond and is epitypified in the present work. Diaporthe neotheicola is reported for the first time on this host. A third species represented by a single isolate could not be unequivocally identified.

show abstract

“…and chestnut blight (caused by Cryphonectria parasitica) are the most important diseases that affect European chestnut, and an upsurge of ink disease has been observed in recent years, probably due to the drier, warmer winters than usual, which have placed the trees under hydric stress (Turchetti and Maresi 2005). In addition to variable climate conditions, fires, abandonment of cultural practices, etc., the decline in productivity of cork oak stands is also associated with pathogens, especially Botryosphaeria stevensii (that causes dieback and trunk canker) and Phytophthora cinnamomi (Luque et al 2002;Alves et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Establishment of cryopreserved gene banks of European chestnut and cork oak

Vidal

Viéitez

Fernández³

et al. 2010

Eur J Forest Res

View full text Add to dashboard Cite

Cryopreservation of selected genotypes of European chestnut and cork oak was carried out in two laboratories in a project involving conservation of field collections. Plant material was selected on the basis of disease resistance (chestnut), growth habit, phytosanitary performance and cork quality (cork oak). The cryopreservation technique comprised of vitrification of shoot apices isolated from in vitro stock shoot cultures (chestnut) and somatic embryos (cork oak). Forty-three out of 46 chestnut genotypes assayed survived the freezing process, but only 63% recovered their capacity to produce new shoots. After completion of multiplication and rooting steps, the surviving shoots produced plants that were morphologically identical to those derived from non-supercooled material. All 51 cork oak genotypes withstood freezing and were able to produce new somatic embryos through a process of secondary embryogenesis. Multiplication and germination of the recovered embryos enabled production of plants that were morphologically identical to those derived from nonsupercooled material. In light of the results obtained, longterm cryopreservation of these species is feasible, thereby ensuring conservation of valuable genotypes during field evaluation.

show abstract

Botryosphaeria corticola, sp. nov. on Quercus Species, with Notes and Description of Botryosphaeria stevensii and Its Anamorph, Diplodia mutila

Cited by 116 publications

References 20 publications

Phylogeny and pathogenicity of Lasiodiplodia species associated with dieback of mango in Peru

Phylogeny and pathogenicity of Lasiodiplodia species associated with dieback of mango in Peru

Phylogeny, morphology and pathogenicity of Diaporthe and Phomopsis species on almond in Portugal

Establishment of cryopreserved gene banks of European chestnut and cork oak

Contact Info

Product

Resources

About