“…Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License Clostridium botulinum, an anaerobic, gram-positive, spore-forming rod commonly found on plants, like vegetables, fruits, and seafood. Numerals of different strains of C. botulinum have been recognized, including seven immunologically distinct serotypes (type A-G) (Archana, 2016;Dutta et al, 2016;Awan, 2017;Mya et al, 2019). Aesthetic use of botulinum toxin type A is one of the most widely studied formulation for cosmetic and therapeutic purposes (Sundaram et al, 2016;Satriyasa, 2019).…”