Both Male and Female Pronuclei Formation in Canine Oocytes Inseminated at Germinal Vesicle Stage.

Shimazu, Yoshiki; Naito, Kunihiko

doi:10.1274/jmor.13.122

Cited by 9 publications

(5 citation statements)

References 6 publications

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“…TO date, there have been no reports of the successful transfer of in vitro matured canine oocytes. Work by Mahi and Yanagimachi (1976), Hewitt (1997) and Shimazu and Naito (1996) showed that dog spermatozoa could penetrate immature homologous oocytes, and recently Fulton and others (1998) fertilised bitch oocytes by intracytoplasmic sperm injection. In the fox, embryos have been produced in vitro, and one subsequently survived to the morula stage (Farstad and others 1993a, b).…”

Section: Figurementioning

confidence: 99%

Pregnancy following in vitro fertilisation of canine oocytes

2001

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The high titres of antibodies to TBE are not always associated with neurological signs. The highest antibody titre (1280) and follow up titres (320) were both recorded in dog 2, which had no clinical signs of neurological disease either before or after the initial examination. Nevertheless, in people, TBE usually has a biphasic course. In the initial phase, lasting one to eight days, the non-specific signs may include vomiting and diarrhoea; this is followed by an asymptomatic period after which 20 to 30 per cent of patients develop neurological symptoms, or the disease resolves without entering the second stage (Kunz 1992). In dog 2, the signs of'gastroenteritis' may represent the initial stage (so far undescribed in dogs), which did not progress to the stage with neurological abnormalities. References

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Section: Figurementioning

confidence: 99%

Pregnancy following in vitro fertilisation of canine oocytes

2001

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“…It is known that the oocytes are ovulated at the germinal vesicle stage and require two to five days for the completion of meiotic maturation (Holst and Phemister 1971, Tsutsui 1975). However, there have been few investigations of IVM, IVF and IVC of canine oocytes (Mahi and Yanagimachi 1976, Yamada and others 1992, 1993, Shimazu and Naito 1996, Hewitt and England 1998, Hewitt and others 1998) in contrast with other mammalian species. This study was conducted to examine the meiotic competence of canine oocytes after co‐culture with bovine cumulus cells for 72 hours, and the competence of these oocytes after IVF.…”

Section: In Vitro Maturation In Vitro Development Number Of Oocytes mentioning

confidence: 99%

“…Yamada and others (1993) and Hewitt and England (1997) demonstrated that the proportion of canine oocytes penetrated by sperm was not affected by the stage of nuclear maturation of the oocyte. Subsequently, Shimazu and Naito (1996) suggested that sperm penetration into the ooplasm promoted the maturation of canine oocytes. In this study, the proportion of oocytes progressing to beyond metaphase I in the co-cultured group (41-6 per cent) was similar to that in the control group (30 9 per cent).…”

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confidence: 99%

Development of canine oocytes matured and fertilised in vitro

et al. 2000

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“…Nevertheless, no report has so far demonstrated this and even the work of Shimazu and Naito has had to rely on speculation that oocytes at the stage of the germinal vesicle take part in normal fertilization [14].…”

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confidence: 99%

Fertilities from Mating and Artificial Insemination with Frozen-Thawed Spermatozoa by Indexing LH Surge in Beagle Bitches.

Shimatsu

Katagiri

Arakawa

et al. 2000

Journal of Reproduction and Development

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Abstract. The purpose of this study was to examine fertilities from mating and artificial insemination (AI) with frozen-thawed spermatozoa by indexing the luteinizing hormone (LH) surge with the Canine Ovulation Timing Test (ICG Status-LH TM ) in beagle bitches. Eleven 2-8-year-old multiparous dogs were used for this experiment and blood was collected daily at 6-15 days after the onset of proestrus. Each serum sample was then used for detection of the LH surge. Five dogs were mated at 5 days after the LH surge. Whelping was successful in all dogs and litter size was 5.8 ± 1.9 (Mean ± SD). The remaining 6 dogs were artificially inseminated with frozen-thawed spermatozoa at 4, 5 and 6 days after the LH surge. Sperm motility after thawing was 49.6 ± 3.6%. All dogs whelped and litter size was 4.0 ± 3.0. These results suggest that fertilities from mating and AI with frozen-thawed spermatozoa are satisfactory by indexing the LH surge with the Canine Ovulation Timing Test in dogs and further demonstrate that the optimal period for insemination is around 5 days after the LH surge.

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Both Male and Female Pronuclei Formation in Canine Oocytes Inseminated at Germinal Vesicle Stage.

Cited by 9 publications

References 6 publications

Pregnancy following in vitro fertilisation of canine oocytes

Pregnancy following in vitro fertilisation of canine oocytes

Development of canine oocytes matured and fertilised in vitro

Fertilities from Mating and Artificial Insemination with Frozen-Thawed Spermatozoa by Indexing LH Surge in Beagle Bitches.

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