“…To confirm expression of functional VP16-RBP-J in VP16-RBP-J rescues, 5 ϫ 10 5 cells were transfected with the following plasmids: 1 g of (RBP-J RE) 12 -Luc or (minimal promoter)-Luc [pGa50-7, firefly luciferase reading frame under the control of a minimal promoter (29)], 0.1 g of phRL-CMV, and 1 g of Bluescript.…”
“…To confirm expression of functional VP16-RBP-J in VP16-RBP-J rescues, 5 ϫ 10 5 cells were transfected with the following plasmids: 1 g of (RBP-J RE) 12 -Luc or (minimal promoter)-Luc [pGa50-7, firefly luciferase reading frame under the control of a minimal promoter (29)], 0.1 g of phRL-CMV, and 1 g of Bluescript.…”
“…The TP1-Luc Notch reporter construct was previously described by Takebayashi et al (1994) and Strobl et al (1997). To perform luciferase-reporter assays, subconfluent cells were cotransfected 24 h after seeding, using Lipofectamine 2000 reagent (Invitrogen) with 0.2 mg of reporter plasmid and 0.05 mg of the pRL-TKRenilla luciferase control plasmid.…”
Section: Plasmid Constructs Cell Transfection and Reporter Assaysmentioning
“…The transforming mechanism, however, di ers since other signaling cascades are modi®ed. In transformations mediated primarily by the Epstein ± Barr virus nuclear antigen 2 (EBNA-2) and related proteins, EBNA-2 transactivates genes in the Notchpathway by interacting with the cellular protein RBP-J kappa (Hsieh et al, 1996;Strobl et al, 1997). The regulation of viral gene expression by cellular factors has been less intensively studied in comparison to human papillomaviruses.…”
Section: Mechanisms By Which Viruses Contribute To Cancermentioning
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