Bosea vaviloviae sp. nov., a new species of slow-growing rhizobia isolated from nodules of the relict species Vavilovia formosa (Stev.) Fed.

Safronova, Vera I.; Kuznetsova, I. G.; Sazanova, Anna L; Kimeklis, Anastasiia K.; Belimov, Andrey A.; Andronov, E. E.; Пинаев, А. Г.; Chizhevskaya, E. P.; Pukhaev, Andrey R.; Popov, K. P.; Willems, Anne; Тихонович, И. А.

doi:10.1007/s10482-015-0383-9

Cited by 54 publications

(32 citation statements)

References 13 publications

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“…Rhizobium gei [3] should be formally transferred to Pararhizobium gei. The rhizobial authentication of the three Bosea species [4,5] may be regarded as pending as none of them was formally demonstrated to induce nodules on legumes so far, nor to harbour known nodulation genes (nodA and nodC could not be amplified) despite being isolated from nodules. As far as we know, Ciceribacter is not confirmed to nodulate, despite having been isolated from the rhizosphere of chickpea.…”

Section: Minute 8 New Speciesmentioning

confidence: 99%

International Committee on Systematics of Prokaryotes Subcommittee for the Taxonomy of Rhizobium and Agrobacterium Minutes of the meeting, Budapest, 25 August 2016

Lajudie

Young

2017

International Journal of Systematic and Evolutionary Microbiology

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Section: Minute 8 New Speciesmentioning

confidence: 99%

International Committee on Systematics of Prokaryotes Subcommittee for the Taxonomy of Rhizobium and Agrobacterium Minutes of the meeting, Budapest, 25 August 2016

Lajudie

Young

2017

International Journal of Systematic and Evolutionary Microbiology

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“…Each strain was isolated from an individual nodule by the standard method (Novikova and Safronova 1992), which was successfully applied for isolation of rhizobia from nodules of different plant species (Safronova et al 2004). Strains were uniformly grown at 28 °C on the modified yeast extract-mannitol agar (YMA) (Vincent 1970) supplemented with 0.5 % succinate (YMSA) (Safronova et al 2015).…”

Section: Bacterial Strains Cultural Conditions and Microscopymentioning

confidence: 99%

“…The primers and PCR conditions for amplification of the 16S rRNA gene and ITS region were described in the previous work (Safronova et al 2015). The direct sequencing of PCR products was performed using an ABI PRISM 3500xl genetic analyzer (Applied Biosystems).…”

Section: Phylogenetic Analysis On the Base Of The Partial 16s Rdna Anmentioning

confidence: 99%

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Extra-slow-growing Tardiphaga strains isolated from nodules of Vavilovia formosa (Stev.) Fed.

et al. 2015

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Eleven extra-slow-growing strains were isolated from nodules of the relict legume Vavilovia formosa growing in North Ossetia (Caucasus) and Armenia. All isolates formed a single rrs cluster together with the type strain Tardiphaga robiniae LMG 26467(T), while the sequencing of the 16S-23S rDNA intergenic region (ITS) and housekeeping genes glnII, atpD, dnaK, gyrB, recA and rpoB divided them into three groups. North Ossetian isolates (in contrast to the Armenian ones) were clustered separately from the type strain LMG 26467(T). However, all isolates were classified as T. robiniae because the DNA-DNA relatedness between them and the type strain LMG 26467(T) was 69.6% minimum. Two symbiosis-related genes (nodM and nodT) were amplified in all isolated Tardiphaga strains. It was shown that the nodM gene phylogeny is similar to that of ITS and housekeeping genes. The presence of the other symbiosis-related genes in described Tardiphaga strains, which is recently described genus of rhizobia, as well as their ability to form nodules on any plants are under investigation.

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“…The rrs similarity of isolates to typical strains B. lathyri LMG26379Т and B. vaviloviae Vaf-18Т was 98.4-99.4 and 99.4-100 %, respectively. It should be noted that species B. vaviloviae was described as recently as 2015 for three microsymbiont strains of a relic legume Vavilovia formosa which grows in North Ossetia [17]. Therefore, Baikal isolates belonging to genus Bosea and having a high index of similarity to species B. vaviloviae are of great interest for further study.…”

mentioning

confidence: 99%

GENETIC DIVERSITY AMONG MICROSYMBIONTS OF Lathyrus, Vicia, Oxytropis AND Astragalus LEGUME SPECIES FROM BAIKAL REGION

Kuznetsova¹,

Sazanova²,

Safronova³

et al. 2015

S-h. biol.

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A b s t r a c t Rhizobia are Gram-negative soil microorganisms that form intracellular nitrogen-fixing symbiosis with leguminous plants. Investigations of symbiotic systems with the participation of endemic or relict species have a particular importance for understanding of the evolution of plantmicrobe interactions. The purpose of our work was to create a representative collection of microsymbionts of endemic Baikal legumes, as well as to estimate their biodiversity. The study of taxonomic positions of 69 isolates from root nodules Lathyrus humilis, Vicia baicalensis, Astragalus mongholicus and Oxytropis sylvatica was conducted. For primary identification of these isolates the methods of ITS-RFLP analysis was used that divided strains into 33 groups with identical DNAprofile. Then the taxonomy positions of isolates were determined by the 16S rRNA gene (rrs) and ITS region sequencing. Phylogenetic analysis revealed the considerable genetic diversity among microsymbionts of plants studied. Rhizobial isolates belonged to 5 genera: Rhizobium (family Rhizobiaceae), Mesorhizobium and Phyllobacterium (family Phyllobacteriaceae), Bosea and Tardiphaga (family Bradyrhizobiaceae). In addition, non-rhizobial isolates belonging to the genera Herbiconiux, Leifsonia, Burkholderia and Stenotrophomonas were obtained. It is known that some species of these genera may be present in the nodules of legumes, but also be inhabitants of rhizosphere or phyllosphere of different plants. The presence of atypical rhizobial microsymbionts in the studied plants was noted, which may indicate the active formation of relationships between partners in the legumerhizobial systems of Baikal region.Keywords: legumes of Baikal region, taxonomy of rhizobia, ribosomal genes sequencing.Nodule bacteria (Rhizobia) belong to a large genetically diverse group of Gram-negative soil microorganisms that form intracellular nitrogen-fixing symbiosis with legumes. One of the urgent tasks of modern biotechnology is the study of mechanisms of interaction of legumes with rhizobia, required for evidence-based selection of highly effective plant-microbe systems [1].Investigations of symbiotic systems with the participation of endemic or relict species have a particular importance for understanding of the evolution of plant-microbe interactions. These unique objects include Baikal legumes, such as low-growing vetchling (Lathyrus humilis), a Late Pleistocene relict of the South Siberian-Severouralsk area [2]; Mongolian milk vetch (Astragalus mongholicus), a rare medicinal plant [3,4]; Baikal endemics Baikal pea (Vicia baicalensis) and forest oxytrope (Oxytropis sylvatica) [5].Few data on Astragalus microsymbionts show a great diversity of these microorganisms belonging to different genera of order Rhizobiales: Rhizobium, Sinorhizobium, Bradyrhizobium,. Vetch-

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Bosea vaviloviae sp. nov., a new species of slow-growing rhizobia isolated from nodules of the relict species Vavilovia formosa (Stev.) Fed.

Cited by 54 publications

References 13 publications

International Committee on Systematics of Prokaryotes Subcommittee for the Taxonomy of Rhizobium and Agrobacterium Minutes of the meeting, Budapest, 25 August 2016

International Committee on Systematics of Prokaryotes Subcommittee for the Taxonomy of Rhizobium and Agrobacterium Minutes of the meeting, Budapest, 25 August 2016

Extra-slow-growing Tardiphaga strains isolated from nodules of Vavilovia formosa (Stev.) Fed.

GENETIC DIVERSITY AMONG MICROSYMBIONTS OF Lathyrus, Vicia, Oxytropis AND Astragalus LEGUME SPECIES FROM BAIKAL REGION

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