Boronic acid recognition of non-interacting carbohydrates for biomedical applications: increasing fluorescence signals of minimally interacting aldoses and sucralose

Abstract

To address carbohydrates that are commonly used in biomedical applications with low binding affinities for boronic acid based detection systems, two chemical modification methods were utilized to increase sensitivity. Modified carbohydrates were analyzed using a two component fluorescent probe based on boronic acid-appended viologen–HPTS (4,4′-o-BBV). Carbohydrates normally giving poor signals (fucose, l-rhamnose, xylose) were subjected to sodium borohydride (NaBH4) reduction in ambient conditions for 1 h yielding the corresponding sugar alcohols from fucose, l-rhamnose and xylose in essentially quantitative yields. Compared to original aldoses, apparent binding affinities were increased 4–25-fold. The chlorinated sweetener and colon permeability marker sucralose (Splenda), otherwise undetectable by boronic acids, was dechlorinated to a detectable derivative by reactive oxygen and hydroxide intermediates by the Fenton reaction or by H2O2 and UV light. This method is specific to sucralose as other common sugars, such as sucrose, do not contain any carbon-chlorine bonds. Significant fluorescence response was obtained for chemically modified sucralose with the 4,4′-o-BBV–HPTS probe system. This proof of principle can be applied to biomedical applications, such as gut permeability, malabsorption, etc.

Electrochemical quantification of Ag2S quantum dots: evaluation of different surface coating ligands for bacteria determination

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Multilocus Distance-Regulated Sensor Array for Recognition of Polyphenols via Machine Learning and Indicator Displacement Assay

“…Traditional techniques for determining tea type, origin, quality, and authenticity primarily rely on stable isotope, mineral element analysis, chromatography, and near-infrared spectroscopy. , However, they face drawbacks including susceptibility to pesticides and contaminants, intricate pretreatment, costly equipment, specialized personnel, and low sensitivity, constraining their broad use. − Cross-responsive optical sensor arrays developed based on the taste system of animals offer unique advantages for detecting multiple targets and mixtures. − These arrays are simple to construct, sensitive, and widely used in food detection. − Our group has been engaged in the development of fluorescent sensor arrays for multiobject detection, such as whiskies, wines, , juices, etc.…”

Construction of a Miniaturized Monosaccharide Detection System Based on Measuring Electric Current and Testing Its Performance Using a Bodipy Fluorescent Dye

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