Boron nitride nanosheet‐assisted matrix solid‐phase dispersion microextraction of alkaloids from lotus plumule by high‐performance liquid chromatography coupled with ultraviolet detection and ion mobility quadrupole time‐of‐flight mass spectrometry

Abstract

A boron nitride nanosheet (BNNS)‐assisted matrix solid‐phase dispersion method was established to microextract alkaloids from medicinal plants. The target compounds were identified by high‐performance liquid chromatography coupled with ultraviolet detection and ion mobility quadrupole time‐of‐flight mass spectrometry. During the experimental process, several important parameters, including the type of dispersant, the amount of dispersant, the grinding time, and the type of elution solvent, were optimized. Finally, the BNNSs were chosen as the best dispersant, and their microcosmic morphologies were identified by scanning electron microscopy and transmission electron microscopy. Because of the special property of BNNSs, the cost of this experiment was greatly reduced, especially in elution volume, sample amount (50 mg), and extraction time (2 min). Under the best conditions, 50 mg of sample powder was dispersed with 50 mg of BNNSs, the grinding time was 120 s, the mixed powder was eluted with 200 μL of methanol, and good linearity (r2 > 0.9993) and satisfactory recoveries (80–100%) were obtained. The inter‐ and intraday precisions were acceptable, with RSDs lower than 2.01 and 4.84%, respectively. The limits of detection ranged from 2.54 to 15.00 ng/mL, and the limits of quantitation were 8.47 to 50.00 ng/mL. The proposed method was successfully applied for the determination of liensinine, isoliensinine, and neferine in lotus plumule.

Application of solid‐phase extraction: High‐resolution mass spectrometry analysis strategy in the characterization and quantification of amide herbicides in aquatic products

“…The complex nature of aquatic product matrices often dictates the requirement for comprehensive sample cleanup prior to successful data acquisition [23]. Common sample cleanup methods include SPE [24,25], the quick, easy, cheap, effective, rugged and safe (QuEChERS) method [26,27], magnetic solid-phase dispersion combined with liquid-liquid extraction [28], displacement fluorescence polarization immunoassay [29], accelerated solvent extraction [30], and dispersion liquid-liquid microextraction [31].…”