Boron Nitride Nanopores: Highly Sensitive DNA Single‐Molecule Detectors

Liu, Song; Lu, Bo; Zhao, Qing; Li, Ji; Gao, Teng; Chen, Yubin; Zhang, Yanfeng; Liu, Zhongfan; Fan, Zhiqin; Yang, Fuhua; You, Long; Yu, Dapeng

doi:10.1002/adma.201301336

Cited by 240 publications

(219 citation statements)

References 28 publications

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“…The first two approaches ultimately increase the sensing length to few nanometers which is not desirable for single nucleotide resolution. An alternative solution is to use other 2D materials such as insulating boron nitride (BN) 16 as the membrane material. To the best of our knowledge, this letter is the first example of utilizing MoS 2 , a newly emerging transition metal dichalcogenide-based material, as a nanopore membrane.…”

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confidence: 99%

Atomically Thin Molybdenum Disulfide Nanopores with High Sensitivity for DNA Translocation

et al. 2014

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Atomically thin nanopore membranes are considered to be a promising approach to achieve single base resolution with the ultimate aim of rapid and cheap DNA sequencing. Molybdenum disulfide (MoS2) is newly emerging as a material complementary to graphene due to its semiconductive nature and other interesting physical properties that can enable a wide range of potential sensing and nanoelectronics applications. Here, we demonstrate that monolayer or few-layer thick exfoliated MoS2 with subnanometer thickness can be transferred and suspended on a predesigned location on the 20 nm thick SiN x membranes. Nanopores in MoS2 are further sculpted with variable sizes using a transmission electron microscope (TEM) to drill through suspended portions of the MoS2 membrane. Various types of double-stranded (ds) DNA with different lengths and conformations are translocated through such a novel architecture, showing improved sensitivity (signal-to-noise ratio >10) compared to the conventional silicon nitride (SiN x ) nanopores with tens of nanometers thickness. Unlike graphene nanopores, no special surface treatment is needed to avoid hydrophobic interaction between DNA and the surface. Our results imply that MoS2 membranes with nanopore can complement graphene nanopore membranes and offer potentially better performance in transverse detection.

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confidence: 99%

Atomically Thin Molybdenum Disulfide Nanopores with High Sensitivity for DNA Translocation

et al. 2014

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“…Mycobacterium smegmatis related protein, Porin A (MspA) can be used to build nanopores which give more resolution between nucleotides (Laszlo et al 2014). Solid state nanopores made up of silicon and carbon compounds, hafnium oxide, molybdenum disulphide, and boron nitride compounds are also recommended for sequencing (Larkin et al 2013;Liu et al 2013a;Venta et al 2013). Solid state nanopores are going to be incorporated in the Optipore sequencing system by NobelGen.…”

Section: Revolutionary and Enduring Sequencing Chemistries And Platformsmentioning

confidence: 99%

Renaissance in phytomedicines: promising implications of NGS technologies

Sharma

Shrivastava

2016

Planta

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Medicinal plant research is growing significantly in faith to discover new and more biologically compatible phytomedicines. Deposition of huge genome/trancriptome sequence data assisted by NGS technologies has revealed the new possibilities for producing upgraded bioactive molecules in medicinal plants. Growing interest of investors and consumers in the herbal drugs raises the need for extensive research to open the facts and details of every inch of life canvas of medicinal plants to produce improved quality of phytomedicines. As in agriculture crops, knowledge emergence from medicinal plant's genome/transcriptome, can be used to assure their amended quality and these improved varieties are then transported to the fields for cultivation. Genome studies generate huge sequence data which can be exploited further for obtaining information regarding genes/gene clusters involved in biosynthesis as well as regulation. This can be achieved rapidly at a very large scale with NGS platforms. Identification of new RNA molecules has become possible, which can lead to the discovery of novel compounds. Sequence information can be combined with advanced phytochemical and bioinformatics tools to discover functional herbal drugs. Qualitative and quantitative analysis of small RNA species put a light on the regulatory aspect of biosynthetic pathways for phytomedicines. Inter or intra genomic as well as transcriptomic interactive processes for biosynthetic pathways can be elucidated in depth. Quality management of herbal material will also become rapid and high throughput. Enrichment of sequence information will be used to engineer the plants to get more efficient phytopharmaceuticals. The present review comprises of role of NGS technologies to boost genomic studies of pharmaceutically important plants and further, applications of sequence information aiming to produce enriched phytomedicines. Emerging knowledge from the medicinal plants genome/transcriptome can give birth to deep understanding of the processes responsible for biosynthesis of medicinally important compounds.

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“…By setting the DNA radius as 1.25 nm, due to the Stern layer effect on DNA solution interface, and associated with other original boundary condition setting, the pore-geometry-variation curves based on a specific openpore current or DNA blockage can be independently mapped. The crossing point of those two curves can determine the pore geometry (pore radius and pore length) at a certain openpore current and DNA blockage [31]. Here, by applying the Poisson-Boltzmann formulation and setting the original boundary conditions, we realize the simulation of pore-geometry-variation curves based on the specific openpore current and protein translocation blockage (Fig.…”

Section: Science China Materialsmentioning

confidence: 99%

“…Nanopore technique has been shown to be a versatile tool for studying size, charge, or folding state of proteins [14,21,[24][25][26] according to the modulation of ionic current caused by their translocation. Solid-state nanopores have prominent advantages of characterizing single molecules including the versatility of pore dimensions, robustness, longer lifetime, and high chemical resistance to denaturing agents [10,[27][28][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Probing surface hydrophobicity of individual protein at single-molecule resolution using solid-state nanopores

Tang

et al. 2015

Sci. China Mater.

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Solid-state nanopore is found to be a promising tool to detect proteins and their complexes. Nanopore-protein interaction is a fundamental and ubiquitous process in biology and medical biotechnology. By translocating phi29 connector protein through silicon nitride nanopores, we demonstrate preliminarily probing the surface hydrophobicity of individual protein at single-molecule resolution. The unique "double-level event" observed in the translocation and the ratio of two current drop levels suggest that the position where the interaction occurs is the hydrophobic surface of the protein. We provide a potential method to locate the hydrophobic region of a specific protein surface. This study is of fundamental significance in revealing the important role that hydrophobic interaction plays in nanopore-protein interaction and holds great potential for detecting local surface chemical property of individual protein using solid-state nanopores.

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Boron Nitride Nanopores: Highly Sensitive DNA Single‐Molecule Detectors

Cited by 240 publications

References 28 publications

Atomically Thin Molybdenum Disulfide Nanopores with High Sensitivity for DNA Translocation

Atomically Thin Molybdenum Disulfide Nanopores with High Sensitivity for DNA Translocation

Renaissance in phytomedicines: promising implications of NGS technologies

Probing surface hydrophobicity of individual protein at single-molecule resolution using solid-state nanopores

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