Border disease virus (BDV) infections of small ruminants in Turkey: A new BDV subgroup?

Oğuzoğlu, Tuba Çiğdem; Tan, Mehmet Tolga; Toplu, N.; Demir, Ayten; Bilge-Dagalp, S.; Karaoğlu, Taner; Özkul, Aykut; Alkan, Feray; Burgu, İbrahim; Haas, Ludwig; Greiser‐Wilke, I.

doi:10.1016/j.vetmic.2008.09.085

Cited by 59 publications

(54 citation statements)

References 20 publications

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“…Further, the high seroprevalence found in this study could be explained by the use of ELISA technique, which is more sensitive that other serological techniques used previously. Our one-step RT-PCR results (Table 3) are similar to those reported by Oguzoglu et al [29].…”

Section: Discussionsupporting

confidence: 91%

Comparative Investigation of Border Disease Virus Infection in Sheep Flocks with Abortion Problems in Konya Province

Avcı

Yavru

2014

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Abstract:The aim of the present study was to determine the serological and virological status of animals persistently infected (PI) with border disease virus (BDV), with focus on the prevalence of pestiviruses in sheep and lambs. This study is the first to our knowledge, to investigate the role of viral infections in abortions in the sheep population of Konya province. The prevalence of antibodies against BDV was as follows: sheep, 79%; lambs, 43.4%; and rams, 6%. Of 1000 leukocyte (sheep) samples examined with a direct ELISA, 11 (1.1%) (first sampling) were antibody-positive. Three sheep were determined to be PI and were sacrificed. Of 1011 sheep leukocyte samples, 14 were examined using a direct immunoperoxidase (IP) assay for BDV antigen, and 11 of 327 (3.36%) tissue samples were positive. The BDV genome was detected in 14 of 63 (22.2%) sheep leukocyte samples and 11 of 327 (3.36%) tissue samples by one-step RT-PCR. The BDV genome could not be detected in any vaginal swab samples. The sensitivity and specificity rates between direct ELISA and direct IP were respectively 100% and 98.8% and the values were, respectively, 78.57% and 98.6% between direct ELISA and one-step RT-PCR and 100% and 97.5% between one-step RT-PCR and direct IP. In conclusion it was determinate that BDV has an important role as an etiologic agent in abortion causes in sheep population in Konya.

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Section: Discussionsupporting

confidence: 91%

Comparative Investigation of Border Disease Virus Infection in Sheep Flocks with Abortion Problems in Konya Province

Avcı

Yavru

2014

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“…In cattle rearing areas of America the rate is 2%, for Argentina as high as 25%, and for United Kingdom as many as 27% of bovine abortions have been associated with pestiviruses, often in conjunction with opportunistic pathogens 18 . The presence of pestiviruses (81.5%) detected in this study is higher than the previously reported studies 6,[9][10][11][12][13][14][15] . Pestiviruses in aborted foetuses are likely to be underdetected for two reasons.…”

Section: Discussioncontrasting

confidence: 81%

“…In conclusion, this study demonstrates percentage of pestiviruses in aborted ruminant foetuses, more than what has been previously reported 6,[9][10][11] . In the future, these results can be deeper reflect due to the studies on molecular typing of pestiviruses.…”

Section: Discussionsupporting

confidence: 58%

“…The presence of pestiviruses were reported between 0.9% and 12.8% in ruminants in different part of Turkey 6,9-16 . The genetic typing of pestiviruses isolated from cattle and sheep in Turkey has shown that a new subgroups of BVDV-1, BVDV-2 and BDV is widespread [10][11][12][13][14] . Morover, the Turkish BVDV-2 field isolates showed the highest similarity with North America BVDV isolates 11 .…”

Section: Discussionmentioning

confidence: 99%

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The Investigation of Pestivirus and Rift Valley Fever Virus Infections in Aborted Ruminant Foetuses in the Blacksea Region in Turkey

Albayrak¹,

Özan²

2012

Kafkas Univ Vet Fak Derg

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SummaryIn this study, aborted foetuses (cattle, sheep and goat) in either coastal or inland Blacksea region of Turkey were surveyed for the presence of nucleic acids from pestiviruses (bovine viral diarrhoea virus-BVDV, border disease virus-BDV) and rift valley fever virus (RVFV) by reverse transcriptase polymerase chain reaction assay (RT-PCR). Pestivirus nucleic acids were found in 39 of 53 aborted calves (73.6%), in 34 of 37 aborted lambs (91.9%) and in 2 of 2 aborted kids (100%) by RT-PCR. In addition RVFV has been investigated in the same foetuses, however RVFV nucleic acid has not been confirmed in any samples. Keywords: Aborted foetus, RT-PCR, Pestivirus, RVFV, Turkey Karadeniz Bölgesinde Ruminant Atık Fötüslerinde Pestivirus ve Rift Vadisi Humması Virus Enfeksiyonlarının Araştırılması ÖzetBu çalışmada, Karadeniz bölgesinin sahil ve iç kesimlerinden elde edilen atık sığır koyun ve keçi fötüsleri pestiviruslar (bovine viral diare virus-BVDV, border hastalığı-BDV) ve rift vadisi humması virus (RVFV) nükleik asitleri yönünden reverz transkriptaz polimeraz zincir reaksiyonu (RT-PZR) ile test edildi. RT-PZR ile 53 atık buzağının 39'unda (%73.6), 37 atık kuzunun 34'ünde (%91.9) ve 2 atık oğlağın tamamında (%100) pestivirus nükleik asidi tespit edildi. Ayrıca aynı atık fötusların tamamında RVFV araştırıldı, fakat hiçbirisinde RVFV nükleik asidi tespit edilemedi.

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“…Ideally, a pestivirus control programme for Turkey should use a vaccine that contains not only BVDV1 strains but also BVDV2. For efficacious BVDV immunization and protection in Turkey, predominant BVDV1 subgenotypes (1-l and 1-f) are clear vaccine candidates (Oguzoglu et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Molecular and serological investigation of border disease virus infection in sheep in the Kars District of Turkey

2014

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This study is a serological and virological examination of the border disease virus (BDV) in sheep at 1-5 years of age from private small scale production units of less than 20 sheep per unit, in the Kars District of Turkey. For this purpose, blood sera from 460 sheep were tested for antibodies against BDV using a commercial enzyme-linked immunosorbent assay (ELISA). Since BDV causes persistent infection, antigen-ELISA was also performed for this agent. Seropositivity rate was detected to be 74.57%. In addition, the BDV antigen was detected in one sample of seronegative sera (0.85%). Reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of pestivirus nucleic acid by using 5'UTR primer pair. Pestivirus nucleic acid was found in 2 of 117 seronegative samples (1.71%) by RT-PCR. The results suggest that the infection was spreading in private small scale production units. Furthermore, recommendations for the control of BDV infection are presented. This study is the first molecular and serological study to determine viroprevalence and seroprevalence of BDV infection in sheep in the Kars District of Turkey.

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Border disease virus (BDV) infections of small ruminants in Turkey: A new BDV subgroup?

Cited by 59 publications

References 20 publications

Comparative Investigation of Border Disease Virus Infection in Sheep Flocks with Abortion Problems in Konya Province

Comparative Investigation of Border Disease Virus Infection in Sheep Flocks with Abortion Problems in Konya Province

The Investigation of Pestivirus and Rift Valley Fever Virus Infections in Aborted Ruminant Foetuses in the Blacksea Region in Turkey

Molecular and serological investigation of border disease virus infection in sheep in the Kars District of Turkey

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