Boosting Geranyl Diphosphate Synthesis for Linalool Production in Engineered Yarrowia lipolytica

Taratynova, Maria O.; Tikhonova, Ekaterina N.; Fedyaeva, Iuliia M.; Dementev, D.A.; Yuzbashev, Tigran V.; Solovyev, Andrey I.; Sineoky, S. P.; Yuzbasheva, Evgeniya Y.

doi:10.1007/s12010-023-04581-z

Cited by 2 publications

(1 citation statement)

References 37 publications

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“…The production of linalool reached 6.96 mg/L in shake flasks. 20 Subsequently, overexpressing multiple copies of Erg20 (FPP synthase) increased the supply of linalool precursor GPP and DGK1 (diacylglycerol kinase) was knocked out by CRSPR-Cas9 technology in Y. lipolytica. The engineered strain accumulated 109.6 mg/L of linalool during cultivation in shake flasks with sucrose as a carbon source.…”

Section: ■ Synthesis Of Terpenoidsmentioning

confidence: 99%

Metabolic Engineering of Yarrowia lipolytica for Efficient Production of Terpenoids

Wang,

Lei,

et al. 2024

Ind. Eng. Chem. Res.

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Yarrowia lipolytica is an unconventional oleaginous yeast with special physiological characteristics. Due to its high flux of acetyl-CoA, extensive substrate spectra, clear gene sequences, and mature genetic modification tools, it has received widespread attention as a chassis strain for the production of various compounds. In this Perspective, we summarize the biosynthetic pathway of terpenoids. The advantages and application prospects of using Y. lipolytica as chassis to produce natural products are introduced. Strategies to further improve the metabolite production efficiency are also discussed, which will provide references and guidance for further expanding application of Y. lipolytica. At present, Y. lipolytica is used to produce a variety of biochemicals. With the development of synthetic biology, the transformation tools of Y. lipolytica can be developed in the future to improve expression capability. It can not only simplify the operation process, but also promote the production yield.

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Section: ■ Synthesis Of Terpenoidsmentioning

confidence: 99%

Metabolic Engineering of Yarrowia lipolytica for Efficient Production of Terpenoids

Wang,

Lei,

et al. 2024

Ind. Eng. Chem. Res.

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Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes

Li,

Liu,

Xiang

et al. 2024

Molecules

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Microbial cell factories, renowned for their economic and environmental benefits, have emerged as a key trend in academic and industrial areas, particularly in the fermentation of natural compounds. Among these, plant-derived terpenes stand out as a significant class of bioactive natural products. The large-scale production of such terpenes, exemplified by artemisinic acid—a crucial precursor to artemisinin—is now feasible through microbial cell factories. In the fermentation of terpenes, two-phase fermentation technology has been widely applied due to its unique advantages. It facilitates in situ product extraction or adsorption, effectively mitigating the detrimental impact of product accumulation on microbial cells, thereby significantly bolstering the efficiency of microbial production of plant-derived terpenes. This paper reviews the latest developments in two-phase fermentation system applications, focusing on microbial fermentation of plant-derived terpenes. It also discusses the mechanisms influencing microbial biosynthesis of terpenes. Moreover, we introduce some new two-phase fermentation techniques, currently unexplored in terpene fermentation, with the aim of providing more thoughts and explorations on the future applications of two-phase fermentation technology. Lastly, we discuss several challenges in the industrial application of two-phase fermentation systems, especially in downstream processing.

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Boosting Geranyl Diphosphate Synthesis for Linalool Production in Engineered Yarrowia lipolytica

Cited by 2 publications

References 37 publications

Metabolic Engineering of Yarrowia lipolytica for Efficient Production of Terpenoids

Metabolic Engineering of Yarrowia lipolytica for Efficient Production of Terpenoids

Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes

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