Book Review: Quorum Sensing vs. Quorum Quenching: A Battle With No End in Sight

Gohil, Nisarg; Panchasara, Happy; Patel, Shreya; Bhattacharjee, Gargi; Singh, Vijai

doi:10.3389/fcimb.2018.00106

Cited by 36 publications

(10 citation statements)

References 20 publications

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“…A serious shortfall of new antibiotics has dragged the world towards global health emergencies [ 2 , 26 ]. In response to defeat this growing threat, a pressing need has arisen to discover novel biocidal substances or to find an alternative approach to eradicate MDR issues [ 4 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is proven that microorganisms communicate with each other via exchange of signalling molecules or auto-inducers through a phenomenon commonly known as quorum-sensing that plays a pivotal role in expressing pathogenicity, motility, biofilm formation, production of antibiotics and pigments, spore formation and many others [ 26 ]. In clinical infections, pathogenic microorganisms cause threats majorly by forming a biofilm.…”

Section: Discussionmentioning

confidence: 99%

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Synergistic bactericidal profiling of prodigiosin extracted from Serratia marcescens in combination with antibiotics against pathogenic bacteria

Gohil

Bhattacharjee

Singh

2020

Microbial Pathogenesis

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The emergence of multidrug-resistant (MDR) bacteria is on the rise and the situation has been worsening with each passing day, which is evident from the outpouring number of reports about how more and more pathogens are becoming resistant to even the third and fourth generations of antibiotics. Lately, combination therapies or drug synergy have been giving promising results in curbing infections since it delineates its action on multiple aspects as compared to monotherapies. In this study, we used prodigiosin, a bacterial pigment endowed with magnificent biological properties, in combination with six antibiotics to study its effect on Pseudomonas aeruginosa , Staphylococcus aureus and Chromobacterium violaceum . Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of prodigiosin against the test organisms was determined and a checkerboard assay of prodigiosin with various antibiotic combinations was performed with an aim to abate antimicrobial resistance. MIC and MBC of prodigiosin was obtained in the range of 4–16 μg/mL, which was lower than that of most test antibiotics. Coupling prodigiosin with other test antibiotics exhibited an excellent synergy profile against all test organisms and the effects were reported to be either synergistic or additive. In the case of S. aureus and C. violaceum , all combinations were found to be synergic, and remarkably for S. aureus , FBC index was reported to be as low as ≤0.25 with all of the test antibiotics. Therefore, it is deduced that prodigiosin augments and intensifies the action of antibiotics, and results in a double-whammy against the MDR strains.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Synergistic bactericidal profiling of prodigiosin extracted from Serratia marcescens in combination with antibiotics against pathogenic bacteria

Gohil

Bhattacharjee

Singh

2020

Microbial Pathogenesis

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“…Although quorum quenching (QQ) is a general term used to describe any form of QS interference, it was specifically coined to relate to the enzymatic quenching of AHL QS signals (Dong et al, 2001). Enzymatic QQ is classified in three main groups: lactonases, oxido-reductases, and acylases (Gohil et al, 2018). They mainly target AHLs and AI-2 mediated QS (Webster, 2012; Hauser et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties

Gupta

Chhibber

2019

Front. Mol. Biosci.

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Background: N-acylated homoserine lactone lactonase which cleave the Acyl homoserine lactone molecules produced by biofilm-forming pathogens and silver nano-particles (AgNPs), are known for their antibacterial effect against several Gram-positive and Gram-negative bacteria. In this study, AgNPs were coated with N-acylated homoserine lactonase protein (AgNPs-AiiA) isolated from Bacillus sp. ZA12. Results: The AgNPs-AiiA complex was characterized by UV-visible spectra, Dynamic light Scattering, Fourier transform infrared spectroscopy (FTIR), and Field Emission Scanning Electron Microscope (Fe-SEM). The synthesized nano-particles were found to be spherical in shape and had an approximate size of 22.4 nm. Treatment with AiiA coated AgNPs showed a significant reduction in exopolysaccharide production, metabolic activity, cell surface hydrophobicity of bacterial cells, and anti-biofilm activity against multidrug-resistant K. pneumoniae as compared to treatment with AiiA protein and neat AgNPs. AgNPs-AiiA complex exhibited potent antibiofilm activity at sub-optimal concentration of 14.4 μg/mL without being harmful to the macrophages and to the various tissues including kidney, liver, spleen and lungs of BALB/c mice upon intra-venous administration. Conclusion: It is concluded that at a concentration of 14.4 μg/mL, AgNPs coated with AiiA kill bacteria without harming the host tissue and provides a suitable template to design novel anti-biofilm drug to circumvent the issue of drug resistance.

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“…etc (Choi et al, 2015; Kumar et al, 2021). This bisindole derivative is biosynthesized by condensation of two L‐tryptophan molecules in response to quorum‐sensing mechanism (Das et al, 2019; Gohil et al, 2018; Gómez‐Gómez et al, 2019). The backbone of violacein is composed of three structural units – an oxindole, a 5‐hydroxyindole and a 2‐pyrrolidone.…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of violacein by Chromobacterium violaceum using agro‐industrial waste soybean meal

Gohil

Bhattacharjee

Gayke

et al. 2021

J of Applied Microbiology

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Aims:The research is aimed at developing an economic and sustainable growth medium using abundantly available and highly nutritive agro-industrial waste soybean meal as the substrate for the production of violacein by Chromobacterium violaceum. Methods and Results:Violacein produced using soybean meal medium was compared with the commercial complex growth media. Upon utilization of 2% w/v soybean meal (SM 2 ) medium, 496 mg/L crude violacein was achieved after 48-hr incubation time, which was 1.62-fold higher than the crude violacein produced in Luria-Bertani (LB) broth. Additionally, supplementation of 100 mg/L L-tryptophan to 1% and 2% w/v soybean meal (SMT 1 and SMT 2 ) medium yielded 1217 mg/L (3.96fold higher as compared to LB) and 1198 mg/L (3.90-fold higher as compared to LB) crude violacein respectively. Optimization of culture conditions and concentration of L-tryptophan using Box-Behnken design (BBD) model produced as high as 1504.5 mg/L crude violacein. To the best of our knowledge, this is the highest crude violacein produced to date using agro-industrial-based waste as a substrate with minimal supplementation in a shake flask. Conclusions:The study signifies the potentiality of soybean meal as a cost-effective growth medium for the production of violacein. Optimization of the fermentation parameters clearly demonstrated a surge in violacein production. Significance and Impact of the Study: Utilization of soybean meal as an alternative to the expensive commercial media would surely promote the large-scale synthesis of this multifaceted compound.

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Book Review: Quorum Sensing vs. Quorum Quenching: A Battle With No End in Sight

Cited by 36 publications

References 20 publications

Synergistic bactericidal profiling of prodigiosin extracted from Serratia marcescens in combination with antibiotics against pathogenic bacteria

Synergistic bactericidal profiling of prodigiosin extracted from Serratia marcescens in combination with antibiotics against pathogenic bacteria

Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties

Enhanced production of violacein by Chromobacterium violaceum using agro‐industrial waste soybean meal

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