Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties

Gupta, Kshitiz; Chhibber, Sanjay

doi:10.3389/fmolb.2019.00063

Cited by 23 publications

(16 citation statements)

References 44 publications

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“…In the present study, AgNPs treated cells showed the percentages of cell surface hydrophobicity were 22.5%, 62.5%, and 73.5% along with 1/2MIC, MIC, and 2MIC, respectively (Figure 7). Gupta and Chhibber (2019) also reported hydrophobicity plummeted sharply in AgNPs treated cells compared to untreated cells. In the present study, result showed reduction in the hydrophobicity in a concentration-dependent manner.…”

Section: Discussionmentioning

confidence: 85%

Antibiofilm Activities of Biogenic Silver Nanoparticles Against Candida albicans

et al. 2022

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Biofilms are microbial colonies that are encased in an organic polymeric matrix and are resistant to antimicrobial treatments. Biofilms can adhere to both biotic and abiotic surfaces, allowing them to colonize medical equipment such as urinary and intravenous catheters, mechanical heart valves, endotracheal tubes, and prosthetic joints. Candida albicans biofilm is the major etiological cause of the pathogenesis of candidiasis in which its unobstructed growth occurs in the oral cavity; trachea, and catheters that progress to systemic infections in the worst scenarios. There is an urgent need to discover novel biofilm preventive and curative agents. In the present investigation, an effort is made to observe the role of cyanobacteria-derived AgNPs as a new antibiofilm agent with special reference to candidiasis. AgNPs synthesized through the green route using Anabaena variabilis cell extract were characterized by UV–visible spectroscopy. The nanoparticles were spherical in shape with 11–15 nm size and were monodispersed. The minimum inhibitory concentration (MIC) of AgNPs was obtained at 12.5 μg/mL against C. albicans. AgNPs 25 μg/mL showed 79% fungal cell membrane permeability and 22.2% ROS production. AgNPs (25 μg/mL) also facilitated 62.5% of biofilm inhibition and degradation. Therefore, AgNPs could be considered as a promising antifungal agent to control biofilm produced by C. albicans.

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Section: Discussionmentioning

confidence: 85%

Antibiofilm Activities of Biogenic Silver Nanoparticles Against Candida albicans

et al. 2022

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“…Size, shape, surface chemistry, chemical composition, surface area and crystal structure are all intrinsic NP properties [9,16,72,83]; ionic strength, pH, the presence of natural organic matter and hardness are media-specific properties that affect toxicity of NPs [48,91]. Biofunctionalized AgNPs are used in many applications [33,73]. The effects of this surface functionalization of NPs on their physicochemical characteristics, fate and toxicity to organisms are critical, but not well understood yet [1,78] which warrants further research.…”

Section: Introductionmentioning

confidence: 99%

The toxicity of coated silver nanoparticles to the alga Raphidocelis subcapitata

et al. 2020

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The use of silver nanoparticles (AgNPs) is growing exponentially, especially in consumer products due to their excellent antimicrobial properties. However, concerns are growing on their possible negative effects on environmental and human health. AgNPs from consumer products enter aquatic ecosystems where their physicochemical properties including surface functionalization are critical to their impact on aquatic organisms. The effects of AgNPs coated with three different ligands; tyrosine (T-AgNP), epigallocatechin gallate (E-AgNP) and curcumin (C-AgNP) and Ag + ions on the freshwater green alga Raphidocelis subcapitata were investigated. Stability tests of AgNPs revealed that the coating significantly affects the fate and behaviour of AgNPs. All types of AgNPs tested and ionic silver were found to be toxic to the alga and differential growth inhibition of algae were observed from differently coated AgNPs, with the 48 h EC 50 of C-AgNPs, T-AgNPs and E-AgNPs being 0.155, 0.163 and 0.243 mg L −1 , respectively, in comparison with 0.051 mg L −1 for Ag + ions. Associated Ag in the algae increased with increased concentrations of all AgNPs and Ag + ions and the toxicity positively correlated to the associated Ag content in algae. The antioxidant enzymes glutathione S-transferase and catalase were activated in algal cells by the AgNPs and Ag + ions, but a consistent difference in response was not identified with different concentrations of NPs. This study shows the effects of the surrounding environment and surface functionalization of AgNPs on algae highlighting the importance of considering them in environmental risk assessment of AgNPs.

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“…The M1 populations are the viable cells that are low in mean Annexin V-FITC fluorescence intensity, while M2 populations are the apoptotic cells that have taken up the Annexin V-FITC and are high in mean Annexin V-FITC fluorescence intensity. Similarly, in a study, the biofunctionalization of Ag nanoparticles with lactonase results in altered cytotoxic properties without harming the host tissue and provides an appropriate template for the design of new antibiofilm drugs to overcome the drug resistance issues (Gupta and Chhibber 2019).…”

Section: Resultsmentioning

confidence: 99%

Functionalization of iron oxide nanoparticles with clove extract to induce apoptosis in MCF-7 breast cancer cells

Thenmozhi

2020

3 Biotech

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It has been reported that iron oxide nanoparticles have various biomedical applications, including cancer diagnosis and treatment. Iron oxide nanoparticles were known to exert cytotoxicity against MCF-7 breast cancer cell lines and in this present study, we have investigated for their apoptosis-inducing potential in the same cell line. The flow cytometry analysis of the MCF-7 breast cancer cell lines treated with functionalized iron oxide nanoparticles showed an increased percentage of cells in terms of viable, early, and late apoptosis. The cell cycle analysis of the MCF-7 cell lines treated with Syzygium aromaticum extract coated with polyvinylpyrrolidone (PVP) + iron oxide nanoparticles and PVP + iron oxide nanoparticles showed substantial accumulation of nanoparticles in the sub-G1 phase, confirming induction of apoptosis. The activities of caspase-3, caspase-8, and caspase-9 increased with increasing concentration of the nanoparticles indicating that activities of caspase can be activated by iron nanoparticles. Further, functionalized nanoparticles induced oxidative stress through reactive oxygen species (ROS) formation. Therefore, it is concluded that the functionalized iron nanoparticles induce apoptosis in MCF-7 breast cancer cell lines and further provides an opportunity to explore the iron nanoparticles for apoptosis in cancer treatment.

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Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties

Cited by 23 publications

References 44 publications

Antibiofilm Activities of Biogenic Silver Nanoparticles Against Candida albicans

Antibiofilm Activities of Biogenic Silver Nanoparticles Against Candida albicans

The toxicity of coated silver nanoparticles to the alga Raphidocelis subcapitata

Functionalization of iron oxide nanoparticles with clove extract to induce apoptosis in MCF-7 breast cancer cells

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