Antibiofilm Activities of Biogenic Silver Nanoparticles Against Candida albicans

Abstract

Biofilms are microbial colonies that are encased in an organic polymeric matrix and are resistant to antimicrobial treatments. Biofilms can adhere to both biotic and abiotic surfaces, allowing them to colonize medical equipment such as urinary and intravenous catheters, mechanical heart valves, endotracheal tubes, and prosthetic joints. Candida albicans biofilm is the major etiological cause of the pathogenesis of candidiasis in which its unobstructed growth occurs in the oral cavity; trachea, and catheters that progress to systemic infections in the worst scenarios. There is an urgent need to discover novel biofilm preventive and curative agents. In the present investigation, an effort is made to observe the role of cyanobacteria-derived AgNPs as a new antibiofilm agent with special reference to candidiasis. AgNPs synthesized through the green route using Anabaena variabilis cell extract were characterized by UV–visible spectroscopy. The nanoparticles were spherical in shape with 11–15 nm size and were monodispersed. The minimum inhibitory concentration (MIC) of AgNPs was obtained at 12.5 μg/mL against C. albicans. AgNPs 25 μg/mL showed 79% fungal cell membrane permeability and 22.2% ROS production. AgNPs (25 μg/mL) also facilitated 62.5% of biofilm inhibition and degradation. Therefore, AgNPs could be considered as a promising antifungal agent to control biofilm produced by C. albicans.

Green Synthesis of Silver Nanoparticles from Camellia sinensis and Its Antimicrobial and Antibiofilm Effect against Clinical Isolates

“…We are also of the opinion that CS-AgNPs arrest the release of exopolysaccharide, which does not allow Candidal cells to adhere onto the surface. Our results are in agreement with the previous studies of Ahamad et al [ 46 ], which revealed the inhibition of Candida albicans biofilm using biogenic silver nanoparticles. Further, Figure 7 represents the interaction of nanoparticles with the fungal cells, and it is very clear that all the nanoparticles are not internalized in the Candida albicans ; rather, few nanoparticles are found at the surface of the Candidal cells (black arrow), whereas some of the nanoparticles have internalized the Candidal cells (red arrow).…”

Therapeutic Effect of Green Synthesized Silver Nanoparticles Using Erodium glaucophyllum Extract against Oral Candidiasis: In Vitro and In Vivo Study

“…In this context, Lara HH et al, in 2015, demonstrated a dose-dependent and potent inhibitory effect of AgNPs on biofilm formation, which were calculated as IC 50 of 0.089 ppm. AgNPs biosynthesized by Anabaena variabilis extract resulted in 62.5% biofilm inhibition and degradation at a concentration of 25 µg/mL [45,59].…”

Metal Nanoparticles to Combat Candida albicans Infections: An Update

“…Antifungal activity against C. albicans planktonic cells and synergism with FCZ have been demonstrated. In a subsequent study, Ahamad and Bano [ 57 ] verified the capacity of AV-AgNPs to inhibit biofilms and suppress the filamentation of C. albicans . C. albicans cells exposed to AV-AgNPs were wrinkled and deformed, with nuclear abnormalities and membrane damage.…”