Bone Tissue Engineering Using Osteogenic Cells: From the Bench to the Clinical Application

Shibli, Jamil Awad; Nagay, B; Suárez, Lina J; Hung, Celeste; Bertolini, Martinna; Barão, Valentim A. R.; Souza, João Gabriel Silva

doi:10.1089/ten.tec.2022.0021

Cited by 20 publications

(14 citation statements)

References 60 publications

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“…Along with the advent of tissue engineering-based approaches, stem cells (SCs) have been applied in an effort to enhance the rate of bone formation and regeneration 18,23,24,34,99–101 . To understand the current research on cell-based engineering for bone tissue regeneration in the CMF region, the cell sources and strategies for osteogenic differentiation and proliferation must be addressed.…”

Section: Cellular Contributions To Bte Constructsmentioning

confidence: 99%

“…Indirect transplantation of SCs in regenerative procedures requires well-defined and efficient protocols to guide their differentiation into the osteogenic cell lineage, not only via selective purification (decreasing the risk of pathogen contamination) but also via controlled proliferation (avoiding spontaneous differentiation of SCs into divergent lineages on transplantation) 23,118,119 . Therefore, factors such as the choice of cell source, isolation procedure, cell preparation and proliferation prior to biomaterial seeding and culturing, as well as costs, safety, and ethical issues, are critical for efficient cell-based BTE therapy 34,120 …”

Section: Cellular Contributions To Bte Constructsmentioning

confidence: 99%

“…4,[10][11][12][13][14][15][16] Moreover, a revolutionary way to stimulate regeneration through loading scaffolds with stem cells (SCs) and/or other osteoprogenitor cells along with growth factors such as platelet concentrates (PCs), bone morphogenetic proteins (BMPs), and certain pharmacological agents, such as dipyridamole (an adenosine A 2A receptor indirect agonist, DIPY) amongst others , have been investigated in preclinical studies. 10,15,23,[33][34][35][36][37][38] Clinical applications of these seeded and/or fabricated 3D-printed scaffolds will be discussed in detail in this review. 21,39…”

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confidence: 99%

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Bone Tissue Engineering (BTE) of the Craniofacial Skeleton, Part I: Evolution and Optimization of 3D-Printed Scaffolds for Repair of Defects

Nayak,

Slavin,

Bergamo

et al. 2023

Journal of Craniofacial Surgery

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Bone tissue regeneration is a complex process that proceeds along the well-established wound healing pathway of hemostasis, inflammation, proliferation, and remodeling. Recently, tissue engineering efforts have focused on the application of biological and technological principles for the development of soft and hard tissue substitutes. Aim is directed towards boosting pathways of the healing process to restore form and function of tissue deficits. Continued development of synthetic scaffolds, cell therapies, and signaling biomolecules seeks to minimize the need for autografting. Despite being the current gold standard treatment, it is limited by donor sites’ size and shape, as well as donor site morbidity. Since the advent of computer-aided design/computer-aided manufacturing (CAD/CAM) and additive manufacturing (AM) techniques (3D printing), bioengineering has expanded markedly while continuing to present innovative approaches to oral and craniofacial skeletal reconstruction. Prime examples include customizable, high-strength, load bearing, bioactive ceramic scaffolds. Porous macro- and micro-architecture along with the surface topography of 3D printed scaffolds favors osteoconduction and vascular in-growth, as well as the incorporation of stem and/or other osteoprogenitor cells and growth factors. This includes platelet concentrates (PCs), bone morphogenetic proteins (BMPs), and some pharmacological agents, such as dipyridamole (DIPY), an adenosine A2A receptor indirect agonist that enhances osteogenic and osteoinductive capacity, thus improving bone formation. This two-part review commences by presenting current biological and engineering principles of bone regeneration utilized to produce 3D-printed ceramic scaffolds with the goal to create a viable alternative to autografts for craniofacial skeleton reconstruction. Part II comprehensively examines recent preclinical data to elucidate the potential clinical translation of such 3D-printed ceramic scaffolds.

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Section: Cellular Contributions To Bte Constructsmentioning

confidence: 99%

Section: Cellular Contributions To Bte Constructsmentioning

confidence: 99%

mentioning

confidence: 99%

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Bone Tissue Engineering (BTE) of the Craniofacial Skeleton, Part I: Evolution and Optimization of 3D-Printed Scaffolds for Repair of Defects

Nayak,

Slavin,

Bergamo

et al. 2023

Journal of Craniofacial Surgery

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“…These membranes can be resorbable or non-resorbable, based on a natural or synthetic composition, and bone grafts [ 1 , 2 ]. Among the many advantages of absorbable–synthetic polymers are the reduction of immunogenicity and the creation of an extracellular matrix similar to the host site that favors cell migration, adhesion, proliferation, and differentiation [ 3 , 4 , 5 , 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Performance of Polydioxanone-Based Membrane in Association with 3D-Printed Bioceramic Scaffolds in Bone Regeneration

et al. 2022

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This study evaluated the bioactivity of 3D-printed β-tricalcium phosphate (β-TCP) scaffolds or hydroxyapatite (HA) scaffolds associated with polydioxanone (PDO) membrane (Plenum® Guide) for guided bone regeneration in rats. Fifty-four rats were divided into three groups (n = 18 animals): autogenous bone + PDO membrane (Auto/PG); 3D-printed β-TCP + PDO membrane (TCP/PG); and 3D-printed HA + PDO membrane (HA/PG). A surgical defect in the parietal bone was made and filled with the respective scaffolds and PDO membrane. The animals were euthanized 7, 30, and 60 days after the surgical procedure for micro-CT, histomorphometric, and immunolabeling analyses. Micro-CT showed an increase in trabecular thickness and a decrease in trabecular separation, even with similar bone volume percentages between TCP/PG and HA/PG vs. Auto/PG. Histometric analysis showed increased bone formation at 30 days in the groups compared to 7 days postoperatively. Immunolabeling analysis showed an increase in proteins related to bone formation at 30 days, and both groups showed a similar immunolabeling pattern. This study concludes that 3D-printed scaffolds associated with PDO membrane (Plenum® Guide) present similar results to autogenous bone for bone regeneration.

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“…Disruption of bone remodeling cycles is the foundation of many bone diseases, including osteoporosis [ 1 ]. In the process of bone remodeling, mesenchymal stem cells (MSCs) differentiate into osteoblasts, which then mature into osteocytes.…”

Section: Introductionmentioning

confidence: 99%

The Roles of SNHG Family in Osteoblast Differentiation

Tan

Zheng

2022

Genes

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Small nucleolar RNA host genes (SNHGs), members of long-chain noncoding RNAs (lncRNAs), have received increasing attention regarding their roles in multiple bone diseases. Studies have revealed that SNHGs display unique expression profile during osteoblast differentiation and that they could act as promising biomarkers of certain bone diseases, such as osteoporosis. Osteogenesis of mesenchymal stem cells (MSCs) is an important part of bone repair and reconstruction. Moreover, studies confirmed that the SNHG family participate in the regulation of osteogenic differentiation of MSCs in part by regulating important pathways of osteogenesis, such as Wnt/β-catenin signaling. Based on these observations, clarifying the SNHG family’s roles in osteogenesis (especially in MSCs) and their related mechanisms would provide novel ideas for possible applications of lncRNAs in the diagnosis and treatment of bone diseases. After searching, screening, browsing and intensive reading, we uncovered more than 30 papers related to the SNHG family and osteoblast differentiation that were published in recent years. Here, our review aims to summarize these findings in order to provide a theoretical basis for further research.

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Bone Tissue Engineering Using Osteogenic Cells: From the Bench to the Clinical Application

Cited by 20 publications

References 60 publications

Bone Tissue Engineering (BTE) of the Craniofacial Skeleton, Part I: Evolution and Optimization of 3D-Printed Scaffolds for Repair of Defects

Bone Tissue Engineering (BTE) of the Craniofacial Skeleton, Part I: Evolution and Optimization of 3D-Printed Scaffolds for Repair of Defects

Performance of Polydioxanone-Based Membrane in Association with 3D-Printed Bioceramic Scaffolds in Bone Regeneration

The Roles of SNHG Family in Osteoblast Differentiation

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