Bone tissue engineering by primary osteoblast-like cells in a monolayer system and 3-dimensional collagen gel

Wiesmann, Hans Peter; Nazer, N.; Klatt, Christoph; Szuwart, Thomas; Meyer, Ulrich

doi:10.1016/j.joms.2003.05.001

Cited by 59 publications

(42 citation statements)

References 36 publications

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“…[1][2][3][4][5][6][7][8][9][10][11][12] The periosteum is a specialized connective tissue that forms a fibrovascular membrane covering all bone surfaces except for that of articular cartilage, muscle, and tendon insertions, and sesamoid bones. 13 Cells residing within the periosteum may be excised from any number of surgically accessible bone surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…15 In addition to their robust proliferation aptitude, it is well established that periosteum-derived progenitor cells have the potential to differentiate into both bone and cartilage. 1,6,8,[10][11][12]14,[16][17][18] Further, their potential for regenerating both bone and cartilage constructs is superior to that of adipose-derived progenitor cells and comparable with that of bone marrow-derived mesenchymal stem cells. 15,19 A recent study by De Bari et al indicates that periosteal progenitor cells are able to differentiate not only into bone and cartilage cells, but also into adipocyte and skeletal myocyte cells.…”

Section: Introductionmentioning

confidence: 99%

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The Periosteum as a Cellular Source for Functional Tissue Engineering

Arnsdorf

Jones

Carter

et al. 2009

Tissue Engineering Part A

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The periosteum, a specialized fibrous tissue composed of fibroblast, osteoblast, and progenitor cells, may be an optimal cell source for tissue engineering based on its accessibility, the ability of periosteal cells to proliferate rapidly both in vivo and in vitro, and the observed differentiation potential of these cells. However, the functional use of periosteum-derived cells as a source for tissue engineering requires an understanding of the ability of such cells to elaborate matrix of different tissues. In this study, we subjected a population of adherent primary periosteum-derived cells to both adipogenic and osteogenic culture conditions. The commitment propensity of periosteal cells was contrasted with that of well-characterized phenotypically pure populations of NIH3T3 fibroblast and MC3T3-E1 osteoblast cell lines. Our results demonstrate that the heterogeneous populations of periosteal cells and NIH3T3 fibroblasts have the ability to express both osteoblast-like and adipocyte-like markers with similar potential. This raises the question of whether fibroblasts within the periosteum may, in fact, have the potential to behave like progenitor cells and play a role in the tissue's multilineage potential or whether there are true stem cells within the periosteum. Further, this study suggests that expanded periosteal cultures may be a source for tissue engineering applications without extensive enrichment or sorting by molecular markers. Thus, this study lays the groundwork for future investigations that will more deeply enumerate the cellular sources and molecular events governing periosteal cell differentiation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Periosteum as a Cellular Source for Functional Tissue Engineering

Arnsdorf

Jones

Carter

et al. 2009

Tissue Engineering Part A

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“…The internalization of fluorescein isothiocyanate (FITC)-labeled CMCht/ PAMAM dendrimer nanoparticles was also investigated using both an osteoblastic cell line (SaOs-2) and RBMSCs as target cells. These types of cells are widely used in tissue engineering approaches [16,17] and are simple in-vitro systems for the assessment of the internalization efficiency of fluorescent-labeled molecules. It is our particular interest to engineer these nanoparticles to find applications in the intracellular controlled delivery of biological agents, including differentiation factors or genetic material, to modulate stem cell behavior while avoiding undesired secondary effects.…”

Section: Introductionmentioning

confidence: 99%

Surface Engineered Carboxymethylchitosan/Poly(amidoamine) Dendrimer Nanoparticles for Intracellular Targeting

Oliveira

Kotobuki

Marques

et al. 2008

Adv Funct Materials

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Novel highly branched biodegradable macromolecular systems have been developed by grafting carboxymethylchitosan (CMCht) onto low generation poly(amidoamine) (PAMAM) dendrimers. Such structures organize into sphere‐like nanoparticles that are proposed to be used as carriers to deliver bioactive molecules aimed at controlling the behavior of stem cells, namely their proliferation and differentiation. The nanoparticles did not exhibit significant cytotoxicity in the range of concentrations below 1 mg mL−1, and fluorescent probe labeled nanoparticles were found to be internalized with highly efficiency by both human osteoblast‐like cells and rat bone marrow stromal cells, under fluorescence‐activated cell sorting and fluorescence microscopy analyses. Dexamethasone (Dex) has been incorporated into CMCht/PAMAM dendrimer nanoparticles and release rates were determined by high performance liquid chromatography. Moreover, the biochemical data demonstrates that the Dex‐loaded CMCht/PAMAM dendrimer nanoparticles promote the osteogenic differentiation of rat bone marrow stromal cells, in vitro. The nanoparticles exhibit interesting physicochemical and biological properties and have great potential to be used in fundamental cell biology studies as well as in a variety of biomedical applications, including tissue engineering and regenerative medicine.

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“…AKP activity is one of the important markers of osteoblast-like cells differentiation and maturity, and reflects the calcification ability of cells and tissues [6].…”

mentioning

confidence: 99%

Effect of Copper on Levels of Collagen and Alkaline Phosphatase Activity from Chondrocytes in Newborn Piglets In Vitro

Yuan

Wang

Zhu

et al. 2011

Biol Trace Elem Res

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The effects of different concentrations of copper on collagen content and alkaline phosphatase (AKP) activity from chondrocytes in newborn piglets were measured. Chondrocytes were cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 μmol/L copper in a 12-well culture plate. Collagen content and AKP activity from the chondrocyte extracellular matrix increased significantly in the culture media with 15.6, 31.2, and 62.5 μmol/L copper and was the highest at 31.2 μmol/L copper (P < 0.05). Thus, the results indicated that copper could promote AKP activity and collagen production by chondrocytes.

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Bone tissue engineering by primary osteoblast-like cells in a monolayer system and 3-dimensional collagen gel

Cited by 59 publications

References 36 publications

The Periosteum as a Cellular Source for Functional Tissue Engineering

The Periosteum as a Cellular Source for Functional Tissue Engineering

Surface Engineered Carboxymethylchitosan/Poly(amidoamine) Dendrimer Nanoparticles for Intracellular Targeting

Effect of Copper on Levels of Collagen and Alkaline Phosphatase Activity from Chondrocytes in Newborn Piglets In Vitro

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