Effect of Copper on Levels of Collagen and Alkaline Phosphatase Activity from Chondrocytes in Newborn Piglets In Vitro

Yuan, Xue‐Feng; Wang, Jianguo; Zhu, Xiaoyan; Zhang, Zhigang; Ai, Yongxing; Sun, Guozhe; Wang, Zhe; Liu, Zhaoxi

doi:10.1007/s12011-011-9151-5

Cited by 23 publications

(8 citation statements)

References 37 publications

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“…(Yassin et al, 2015;Chen et al, 2020). Furthermore, copper supplementation in meals has been shown to reduce the severity of osteoarthritis and other developing cartilage lesions, owing to improved collagen cross-linking and increased collagen type II production (Yuan et al, 2011). Also, the obtained results agreed with previous reviews as it shows reduction in aggrecan immunostaining intensity was less pronounced in both mild and severe osteoarthritis treated group.…”

Section: Discussionsupporting

confidence: 83%

Ameliorative Effect of Copper Albumin Complex on Proteoglycan in Mono-iodoacetat Induced Osteoarthritis Rat Model

Shabaan

Mostafa

El‐Mahdy

et al. 2022

SVU-International Journal of Veterinary Sciences

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Osteoarthritis (OA) is a degenerative disorder involving the joint, including cartilage and synovial fluids. Recent studies have sought to find curative therapeutics to decrease the adverse effects of OA and relieve associated pain. Globally, knee osteoarthritis (KOA) is the most common type of arthritis. The present study aimed to evaluate the anti-inflammatory effect copper albumen complex (cu-albumin complex) for the treatment of mono-iodoacetate (MIA)induced KOA in Albino rats. A total of 50 adult male albino rats were involved and divided as follows; 10 rats were kept normal as negative control; 20 arthritic rats were kept untreated (positive control), and 20 arthritic rats were treated with cu-albumin complex orally for a month. Treated and untreated arthritic rats were divided equally (10 rats each) into mild and severe groups according to the severity of signs. The intra-articular injection of MIA in the right knee joint was used for induction of osteoarthritis. Using Mankin grading score, the results demonstrated that the treated groups had a better histological appearance than the control positive group. Additionally, except for a few shrunken chondrocytes, the mildly treated group showed less degenerative alterations and appeared virtually normal. While the severe treated group showed increased cellularity with decreased degenerated chondrocytes. It concluded that balanced copper consumption has a positive impact on the prevention and treatment of KOA.

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Section: Discussionsupporting

confidence: 83%

Ameliorative Effect of Copper Albumin Complex on Proteoglycan in Mono-iodoacetat Induced Osteoarthritis Rat Model

Shabaan

Mostafa

El‐Mahdy

et al. 2022

SVU-International Journal of Veterinary Sciences

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“…Supplementation of dietary copper has been reported to reduce the severity of osteochondrosis and other developmental cartilage lesions, which may result from enhanced collagen cross-linking and increased collagen II synthesis (Knight et al, 1990;Hurtig et al, 1993;Yuan et al, 2011). The chondroprotection efficacy of copper may be attributable to the anti-catabolic effects of Cu 2+ , which abrogates the degradation of cartilage matrix proteoglycan via inhibition of nitric oxide release (Pasqualicchio et al, 1996).…”

Section: Chondroprotective Effects Of Copper In Cartilagementioning

confidence: 99%

Molecular Insights Into Lysyl Oxidases in Cartilage Regeneration and Rejuvenation

Lin

2020

Front. Bioeng. Biotechnol.

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Articular cartilage remains among the most difficult tissues to regenerate due to its poor self-repair capacity. The lysyl oxidase family (LOX; also termed as protein-lysine 6oxidase), mainly consists of lysyl oxidase (LO) and lysyl oxidase-like 1-4 (LOXL1-LOXL4), has been traditionally defined as cuproenzymes that are essential for stabilization of extracellular matrix, particularly cross-linking of collagen and elastin. LOX is essential in the musculoskeletal system, particularly cartilage. LOXs-mediated collagen cross-links are essential for the functional integrity of articular cartilage. Appropriate modulation of the expression or activity of certain LOX members selectively may become potential promising strategy for cartilage repair. In the current review, we summarized the advances of LOX in cartilage homeostasis and functioning, as well as copper-mediated activation of LOX through hypoxia-responsive signaling axis during recent decades. Also, the molecular signaling network governing LOX expression has been summarized, indicating that appropriate modulation of hypoxia-responsive-signaling-directed LOX expression through manipulation of bioavailability of copper and oxygen is promising for further clinical implications of cartilage regeneration, which has emerged as a potential therapeutic approach for cartilage rejuvenation in tissue engineering and regenerative medicine. Therefore, targeted regulation of copper-mediated hypoxiaresponsive signalling axis for selective modulation of LOX expression may become potential effective therapeutics for enhanced cartilage regeneration and rejuvenation in future clinical implications.

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“…Gupta [63] believed that copper inhibited acid phosphatase activity and alkaline phosphatase activity, and in turn greatly inhibited RNA synthesis. Yuan's study [64] indicated that copper could promote AKP activity. The significant difference was observed after eight days' incubation and the highest AKP value was obtained in the culture media with 31.2 μmol L −1 (4.99 mg L −1 ) copper ions; and furthermore Cu ion reduced the AKP [64].…”

Section: Discussionmentioning

confidence: 99%

The antibacterial properties and biocompatibility of a Ti–Cu sintered alloy for biomedical application

et al. 2014

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The antibacterial activity, the cytotoxicity and the cell function of a sintered Ti-10 wt% Cu alloy were investigated in order to assess the suitability of the alloy for biomedical application. The antibacterial activity of the alloy was investigated by a plate-count method and the cytotoxicity was studied by examining the MG63 cell response by CCK8 assessment. The cell function was monitored by measuring the AKP activity. The Cu ion released from the Ti-Cu alloy was also measured by an inductively coupled plasma spectrometer at different immersion durations. The results show that the antibacterial rates of the alloy against Escherichia coli and Staphylococcus aureus increase with an increase in the incubation duration. After 7 h of incubation, the alloy showed an antibacterial rate of 91.66% against S. aureus and 99. 01% against E. coli. With a further extension of incubation time to 24 h, the antibacterial rate increased to 100% against S. aureus and 99.93% against E. coli. No cytotoxicity was observed on the alloy by a CKK8 test during three days of incubation in comparison with commercially available pure titanium (cp-Ti). AKP test results showed a significantly high AKP value (p = 0.001 < 0.01) on the Ti-Cu alloy on day 1. The Cu ion release was thought to contribute to the strong antibacterial property, but the Cu ion did not lead to cell cytotoxicity. Strong antibacterial activity and good cell biocompatibility suggest that the Ti-Cu alloy could reduce bacterial infection and have a potential application as an implant material.

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Effect of Copper on Levels of Collagen and Alkaline Phosphatase Activity from Chondrocytes in Newborn Piglets In Vitro

Cited by 23 publications

References 37 publications

Ameliorative Effect of Copper Albumin Complex on Proteoglycan in Mono-iodoacetat Induced Osteoarthritis Rat Model

Ameliorative Effect of Copper Albumin Complex on Proteoglycan in Mono-iodoacetat Induced Osteoarthritis Rat Model

Molecular Insights Into Lysyl Oxidases in Cartilage Regeneration and Rejuvenation

The antibacterial properties and biocompatibility of a Ti–Cu sintered alloy for biomedical application

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