Bone regeneration with BMP-2 and hydroxyapatite in critical-size calvarial defects in rats

Notodihardjo, Frederik Zefanya; Kakudo, Natsuko; Kushida, Satoshi; Suzuki, Kenji; Kusumoto, Kenji

doi:10.1016/j.jcms.2011.04.008

Cited by 62 publications

(39 citation statements)

References 35 publications

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“…However, both Biomaterial and Biomaterial + Cells groups presented similar behavior: in both groups, it was possible to verify cells and new bone formation into natural pores of the granules, this last similar to those described in the literature (22). These results are interesting because these materials can be used as scaffold to osteoblasts in addition to promoting the recruitment of cells from the host tissue (22,23).…”

Section: Discussionsupporting

confidence: 75%

“…However, a recent study reported no significant improvement after the addition of cells (osteoblasts and osteoprogenitor cells) and/or growth factors when compared with the biomaterial alone (21), results that are similar to the present ones. Moreover, most studies in the literature have shown that the addition of cells, in combination or not with growth factors and transported by a biomaterial may favor the process of bone formation (22). Some studies have reported an increase in bone formation, accelerated by these components (cells and growth factors) in the initial period or shorter (1-2 weeks) (23).…”

Section: Discussionmentioning

confidence: 99%

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Exploring anorganic bovine bone granules as osteoblast carriers for bone bioengineering: a study in rat critical-size calvarial defects

Zambuzzi¹,

Fernandes

Iano³

et al. 2012

Braz. Dent. J.

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It is known that current trends on bone bioengineering seek ideal scaffolds and explore innovative methods to restore tissue function. In this way, the objective of this study was to evaluate the behavior of anorganic bovine bone as osteoblast carrier in critical-size calvarial defects. MC3T3-E1 osteoblast cells (1x10 5 cells/well) were cultured on granules of anorganic bovine bone in 24-well plates and after 24 h these granules were implanted into rat critical-size calvarial defects (group Biomaterial + Cells). In addition, other groups were established with different fillings of the defect: Blood Clot (negative control); Autogenous Bone (positive control); Biomaterial (only granules) and Cells (only MC3T3-E1 cells). After 30 days, the animals were euthanized and the calvaria were technically processed in order to allow histological and morphometric analysis. It was possible to detect blood vessels, connective tissue and newly formed bone in all groups. Particularly in the Biomaterial + Cells group, it was possible to observe a profile of biological events between the positive control group (autogenous bone) and the group in which only anorganic bovine granules were implanted. Altogether, the results of the present study showed that granules of anorganic bovine bone can be used as carrier to osteoblasts and that adding growth factors at the moment of implantation should maximize these results.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Exploring anorganic bovine bone granules as osteoblast carriers for bone bioengineering: a study in rat critical-size calvarial defects

Zambuzzi¹,

Fernandes

Iano³

et al. 2012

Braz. Dent. J.

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“…HA-chitosan scaffolds have shown good cytocompatibilty with osteoblasts which is an indication for HA to be used in bone tissue engineering applications [27, 28]. Additionally, substantial research has shown that presence of HA in bone scaffolds could improve the differentiation of pre-osteoblasts, human mesenchymal stem cells and vascular smooth muscle cells to osteoblasts in the presence of osteogenic factors [29-31]. …”

Section: Introductionmentioning

confidence: 99%

Injectable porous nano-hydroxyapatite/chitosan/tripolyphosphate scaffolds with improved compressive strength for bone regeneration

Uswatta

Okeke

Jayasuriya

2016

Materials Science and Engineering: C

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In this study we have fabricated porous injectable spherical scaffolds using chitosan biopolymer, sodium tripolyphosphate (TPP) and nano-hydroxyapatite (nHA). TPP was primarily used as an ionic crosslinker to crosslink nHA/chitosan droplets. We hypothesized that incorporating nHA into chitosan could support osteoconduction by emulating the mineralized cortical bone structure, and improve the Ultimate Compressive Strength (UCS) of the scaffolds. We prepared chitosan solutions with 0.5%, 1% and 2% (w/v) nHA concentration and used simple coacervation and lyophilization techniques to obtain spherical scaffolds. Lyophilized spherical scaffolds had a mean diameter of 1.33 mm (n=25). Further, portion from each group lyophilized scaffolds were soaked and dried to obtain Lyophilized Soaked and Dried (LSD) scaffolds. LSD scaffolds had a mean diameter of 0.93 mm (n=25) which is promising property for the injectability. Scanning Electron Microscopy images showed porous surface morphology and interconnected pore structures inside the scaffolds. Lyophilized and LSD scaffolds had surface pores less than 10 and 2 µm, respectively. 2% nHA/chitosan LSD scaffolds exhibited UCS of 8.59 MPa compared to UCS of 2% nHA/chitosan lyophilized scaffolds at 3.93 MPa. Standardize UCS values were 79.98 MPa and 357 MPa for 2% nHA/chitosan lyophilized and LSD particles respectively. One-way ANOVA results showed a significant increase (p < 0.001) in UCS of 1% and 2% nHA/chitosan lyophilized scaffolds compared to 0% and 0.5% nHA/chitosan lyophilized scaffolds. Moreover, 2% nHA LSD scaffolds had significantly increased (p < 0.005) their mean UCS by 120% compared to 2% nHA lyophilized scaffolds. In a drawback, all scaffolds have lost their mechanical properties by 95% on the 2nd day when fully immersed in phosphate buffered saline. Additionally live and dead cell assay showed no cytotoxicity and excellent osteoblast attachment to both lyophilized and LSD scaffolds at the end of 14th day of in vitro studies. 2% nHA/chitosan scaffolds showed higher osteoblast attachment than 0% nHA/chitosan scaffolds.

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“…[20][21][22][23] Furthermore, in the control group, the healing was characterized with the collagen fibers and high amounts of fibroblasts in the connective tissue. Bone formation was observed only in the edges of defect.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Allograft Combined with Ozone Therapy on Regeneration of Calvarial Defects in Rats

Toker

Özdemir²,

Kuzu³

et al. 2017

Cumhuriyet Dental Journal

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Objective: Ozone accelerates wound healing and increases oxygen supply. The purpose of this study was to investigate the effect of ozone therapy combined with bone allograft on bone regeneration in rats with calvarial defects, histomorphometrically and histopathologically.Material-Method: Twenty four male Wistar rats were used in this study. A 5 mm diameter critical-size defects were created in all rats using a trephine bur. The rats were divided into 4 groups; empty defect (control) (n=6), ozone application into the control defect (control+ ozone) group (n=6), defect filled with allograft (Allograft) group (n=6), ozone combined with allograft application (allograft + ozone) group (n=6). Gaseous ozone administered at 80% density for 14 days. The animals were euthanized at 8-week. Total bone area was measured histomorphometrically and osteoclast and osteoblast number were analyzed histopathologically. Results:The osteoblast numbers increased in allograft + ozone group compared to the allograft group (p<0.05). The control defects showed significantly less new bone formation than those of the allograft groups at 8 weeks (p<0.05). Total bone area was significantly higher in allograft+ozone group than those of the allograft group (p<0.05).Conclusions: Within the limitations of this study, we suggested that allograft+ozone combination increased osteoblast number and new bone area. However, further studies are needed to investigate the mechanisms of action of ozone on bone regeneration.

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Bone regeneration with BMP-2 and hydroxyapatite in critical-size calvarial defects in rats

Cited by 62 publications

References 35 publications

Exploring anorganic bovine bone granules as osteoblast carriers for bone bioengineering: a study in rat critical-size calvarial defects

Exploring anorganic bovine bone granules as osteoblast carriers for bone bioengineering: a study in rat critical-size calvarial defects

Injectable porous nano-hydroxyapatite/chitosan/tripolyphosphate scaffolds with improved compressive strength for bone regeneration

The Effects of Allograft Combined with Ozone Therapy on Regeneration of Calvarial Defects in Rats

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