Bone regeneration using collagen type I vitrigel with bone morphogenetic protein-2

Zhao, Jiyuan; Shinkai, Masashige; Takezawa, Toshiaki; Ohba, Shinsuke; Chung, Ung‐il; Nagamune, Teruyuki

doi:10.1016/j.jbiosc.2008.10.007

Cited by 51 publications

(34 citation statements)

References 25 publications

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“…6 (c)). Expression of OCN, a marker of late stage osteoblastic differentiation 14) , was not observed on days 7 in hMSCs cultured on the BAp and HAp membranes. However, by days 14, hMSCs cultured on the BAp membrane had higher OCN expression than did hMSCs cultured on the HAp membrane (Fig.…”

Section: Resultsmentioning

confidence: 86%

“…Real-time RT-PCR analysis showed that hMSCs cultured expressed the osteogenic marker runx2, which is known to be an early stage marker of osteogenesis. OCN is recognized as a marker of late stage osteoblastic differentiation 14) . In osteoblastic cells, transcription of the bone-specific OCN gene is principally regulated by the runx2 transcription factor 22) .…”

Section: Discussionmentioning

confidence: 99%

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A novel membrane-type apatite scaffold engineered by pulsed laser ablation

et al. 2015

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Cell sheet technology is a scaffold-free method for tissue reconstruction. A sheet-shaped scaffold would be suitable for the regeneration of periodontal membrane. We designed a stem cell sheet combining human mesenchymal stromal cells (hMSCs) and a 10-µm thick biological apatite (BAp) membrane fabricated with an ArF pulsed laser ablation for periodontal regeneration. X-ray diffraction showed that crystalline hydroxyapatite (HAp) was present in BAp and HAp membranes after post-annealing. Energy dispersive analysis of the BAp membrane revealed peaks of Na and Mg in addition to Ca and P. Approximately 3×10 4 hMSCs were cultured on BAp and HAP membranes for 7 and 14 days. From in vitro assays, hMSCs grew faster and had higher osteoblast differentiation when cultured on the BAp membrane than did the cell culture on the HAp membrane. Stem cell sheets combined with a BAp membrane may have potential applications in guided bone regeneration and osteoconductive scaffolds.

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Section: Resultsmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

A novel membrane-type apatite scaffold engineered by pulsed laser ablation

et al. 2015

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“…DNA content, as an indicator of cell proliferation, also increased. DFAT cells differentiated into osteoblasts in TFM because osteocalcin (Zhao et al 2009) and Fig. 3 Scanning electron microscopy analysis.…”

Section: Discussionmentioning

confidence: 99%

Dedifferentiated fat cells differentiate into osteoblasts in titanium fiber mesh

et al. 2012

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Mature adipocyte-derived dedifferentiated fat (DFAT) cells rapidly differentiate into osteoblasts under three-dimensional culture conditions. However, it has not been demonstrated that DFAT cells can differentiate into osteoblasts in a rigid scaffold consisting of titanium fiber mesh (TFM). We examined the proliferation and osteogenic differentiation ability of DFAT cells using TFM as a scaffold. DFAT cells derived from rabbit subcutaneous fat were seeded into TFM and cultured in osteogenic medium containing dexamethasone, L-ascorbic acid 2-phosphate and b-glycerophosphate for 14 days. In scanning electron microscopy (SEM) analysis, well-spread cells covered the titanium fibers on day 3, and appeared to increase in number from day 3 to 7. Numerous globular accretions were found and almost completely covered the fibers on day 14. Cell proliferation, as measured by DNA content in the TFM, was significantly higher on day 7 compared with that of day 1. Osteocalcin and calcium content in the TFM were significantly higher on day 14 compared to those of days 1, 3, and 7, indicating DFAT cells differentiated into osteoblasts. We theorize that globular accretions observed in SEM analysis may be calcified matrix resulting from osteocalcin secreted by osteoblasts binding calcium contained in fetal bovine serum. In this study, we demonstrated that DFAT cells differentiate into osteoblasts and deposit mineralized matrices in TFM. Therefore, the combination of DFAT cells and TFM may be an attractive option for bone tissue engineering.

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“…One alternative that is designed to extend the sustained release of GFs and enhance the mechanical properties is obtained by converting a soft, turbid disk of type I collagen gel into a strong, transparent vitrigel membrane using a concept for the vitrification of heat-denatured protein membranes [54][55][56]. Gelatin is a denatured collagen that is currently used in GFDS.…”

Section: Natural Polymersmentioning

confidence: 99%