Dedifferentiated fat cells differentiate into osteoblasts in titanium fiber mesh

Kishimoto, Naotaka; Momota, Yoshihiro; Hashimoto, Yoshiya; Ando, Kayoko; Ômasa, Takeshi; Kotani, Junichiro

doi:10.1007/s10616-012-9456-z

Cited by 33 publications

(30 citation statements)

References 25 publications

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“…When examined for their differentiation potentials in vitro, DFAT cells exhibit differentiation similar to their ASC counterparts. For example, all-trans retinoic acid can induce the osteogenic differentiation of DFAT cells [399] and increased osteocalcin expression and calcium deposition is observed when DFAT cells are seeded onto titanium fiber meshes [400]. Moreover, osteogenically induced DFAT cells are also capable of forming osteoid when subcutaneously implanted in athymic mice [396].…”

Section: Dedifferentiated Fat Cells (Dfat)mentioning

confidence: 99%

Adipose-Derived Stem Cells in Tissue Regeneration: A Review

2013

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In 2001, researchers at the University of California, Los Angeles, described the isolation of a new population of adult stem cells from liposuctioned adipose tissue. These stem cells, now known as adipose-derived stem cells or ADSCs, have gone on to become one of the most popular adult stem cells populations in the fields of stem cell research and regenerative medicine. As of today, thousands of research and clinical articles have been published using ASCs, describing their possible pluripotency in vitro, their uses in regenerative animal models, and their application to the clinic. This paper outlines the progress made in the ASC field since their initial description in 2001, describing their mesodermal, ectodermal, and endodermal potentials both in vitro and in vivo, their use in mediating inflammation and vascularization during tissue regeneration, and their potential for reprogramming into induced pluripotent cells.

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Section: Dedifferentiated Fat Cells (Dfat)mentioning

confidence: 99%

Adipose-Derived Stem Cells in Tissue Regeneration: A Review

2013

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“…Furthermore, subcutaneous transplanted DFAT cells formed ectopic osteoid tissue in C57BL/6N mice in vivo only with all- trans retinoic acid. Kishimoto et al 43 demonstrated rabbit derived DFAT cells differentiated into osteoblasts in a rigid scaffold consisting of titanium fiber mesh, providing an experimental basis for bone regeneration therapy.…”

Section: Multipotent Capacity Of Dfat Cellsmentioning

confidence: 99%

Dedifferentiated adipocyte-derived progeny cells (DFAT cells)

et al. 2013

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Analyses of mature adipocytes have shown that they possess a reprogramming ability in vitro, which is associated with dedifferentiation. The subsequent dedifferentiated fat cells (DFAT cells) are multipotent and can differentiate into adipocytes and other cell types as well. Mature adipocytes can be easily obtained by biopsy, and the cloned progeny cells are homogeneous in vitro. Therefore, DFAT cells (a new type of stem cell) may provide an excellent source of cells for tissue regeneration, engineering and disease treatment. The dedifferentiation of mature adipocytes, the multipotent capacity of DFAT cells and comparisons and contrasts with mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPS) are discussed in this review.

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“…Among recent studies to explore DFAT cells, specific purification steps to insure the purity of the initial culture of mature adipocytes has seldom been reported [4][5][6][7][8][9][10]. Indeed, 96%-100% purity of isolated mature adipocyte samples utilizing fluorescence activated cell sorting (FACS), immunostaining or confocal microscopy was reported in several studies [1,[11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%