Bone regeneration capacities of alveolar bone mesenchymal stem cells sheet in rabbit calvarial bone defect

Liu, Yanan; Wang, Haifeng; Dou, Huixin; Tian, Bin; Li, Le; Jin, Luyuan; Zhang, Zhenting; Hu, Lei

doi:10.1177/2041731420930379

Cited by 32 publications

(30 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition to the collagen based artificial scaffolds or membranes, it is worthy to mention another promising type of scaffold, based more on the extracellular matrix as well as cells, named as “cell sheet” ( Chen et al, 2019 ; Lu et al, 2019 ). Cell sheet was efficaciously utilized in the regeneration of bone and periodontal ligament-like tissues as shown by recent works ( Dong et al, 2020 ; Liu Y. et al, 2020 ). Promising as it demonstrated to be, cell sheet manufacture requires high manipulation skills to ensure the quality control ( Chen et al, 2019 ), while the current artificial Col/PVA membrane offers the superiority in relatively easy manufacturing with controllability.…”

Section: Resultsmentioning

confidence: 99%

Fabrication and Characterization of Collagen/PVA Dual-Layer Membranes for Periodontal Bone Regeneration

Zhou

Chen

Ding

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Guided tissue regeneration (GTR) is a promising treatment for periodontal tissue defects, which generally uses a membrane to build a mechanical barrier from the gingival epithelium and hold space for the periodontal regeneration especially the tooth-supporting bone. However, existing membranes possess insufficient mechanical properties and limited bioactivity for periodontal bone regenerate. Herein, fish collagen and polyvinyl alcohol (Col/PVA) dual-layer membrane were developed via a combined freezing/thawing and layer coating method. This dual-layer membrane had a clear but contact boundary line between collagen and PVA layers, which were both hydrophilic. The dual membrane had an elongation at break of 193 ± 27% and would undergo an in vitro degradation duration of more than 17 days. Further cell experiments showed that compared with the PVA layer, the collagen layer not only presented good cytocompatibility with rat bone marrow-derived mesenchymal stem cells (BMSCs), but also promoted the osteogenic genes (RUNX2, ALP, OCN, and COL1) and protein (ALP) expression of BMSCs. Hence, the currently developed dual-layer membranes could be used as a stable barrier with a stable degradation rate and selectively favor the bone tissue to repopulate the periodontal defect. The membranes could meet the challenges encountered by GTR for superior defect repair, demonstrating great potential in clinical applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Fabrication and Characterization of Collagen/PVA Dual-Layer Membranes for Periodontal Bone Regeneration

Zhou

Chen

Ding

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…A recent study demonstrated that adult manidular skeletal stem cells activated an embryonic NC cell‐like gene regulatory program that is dependent on the focal adhesion kinase pathway during distraction osteogenesis, 120 enabling these cells to acquire a more plastic, developmental state that aids in jaw bone regeneration. In addition, alveolar bone‐derived MSCs 121 display high osteogenic potential and promote ectopic bone formation in vivo, 122–125 providing a more accessible MSC source compared with the iliac crest.…”

Section: Introductionmentioning

confidence: 99%

Neural crest-like stem cells for tissue regeneration

Soto

Ding

Wang

et al. 2021

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Neural crest stem cells (NCSCs) are a transient population of cells that arise during early vertebrate development and harbor stem cell properties, such as self‐renewal and multipotency. These cells form at the interface of non‐neuronal ectoderm and neural tube and undergo extensive migration whereupon they contribute to a diverse array of cell and tissue derivatives, ranging from craniofacial tissues to cells of the peripheral nervous system. Neural crest‐like stem cells (NCLSCs) can be derived from pluripotent stem cells, placental tissues, adult tissues, and somatic cell reprogramming. NCLSCs have a differentiation capability similar to NCSCs, and possess great potential for regenerative medicine applications. In this review, we present recent developments on the various approaches to derive NCLSCs and the therapeutic application of these cells for tissue regeneration.

show abstract

“…Although it has been reported that CHI3L1 is not synthesized in undifferentiated iliac MSCs [ 37 ], some undifferentiated MBMSCs synthesized and secreted CHI3L1 in this study. MBMSCs have lower chondrogenic and adipogenic differentiation capacity [ 9 ] and greater osteogenic differentiation capacity than iliac MSCs [ 38 , 39 ]. In addition, MBMSCs and iliac MSCs have been reported to have different gene expression according to DNA microarray analysis [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of chitinase-3-like protein 1 as an in vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells

Komabashiri

Suehiro

Ishii

et al. 2021

Regenerative Therapy

View full text Add to dashboard Cite

Introduction Maxillary/mandibular bone marrow stromal cells (MBMSCs) are a useful cell source for bone regeneration in the oral and maxillofacial region. To further ensure the clinical application of MBMSCs in bone regenerative therapy, it is important to determine the bone formation capacity of MBMSCs before transplantation. The aim of this study is to identify the molecular marker that determines the in vivo bone formation capacity of MBMSCs. Methods The cell growth, cell surface antigens, in vitro and in vivo bone formation capacity of MBMSCs were examined. The amount of chitinase-3-like protein 1 (CHI3L1) secreted into the conditioned medium was quantified. The effects of CHI3L1 on the cell growth and osteogenic differentiation potential of MBMSCs and on the cell growth and migration of vascular endothelial cells and fibroblasts were examined. Results The cell growth, and in vitro and in vivo bone formation capacity of the cells treated with different conditions were observed. MBMSCs that secreted a large amount of CHI3L1 into the conditioned medium tended to have low in vivo bone formation capacity, whereas MBMSCs that secreted a small amount of CHI3L1 had greater in vivo bone formation capacity. CHI3L1 promoted the migration of vascular endothelial cells, and the cell growth and migration of fibroblasts. Conclusion Our study indicates that the in vitro osteogenic differentiation capacity of MBMSCs and the in vivo bone formation capacities of MBMSCs were not necessarily correlated. The transplantation of high CHI3L1 secretory MBMSCs may suppress bone formation by inducing fibrosis at the site. These results suggest that the CHI3L1 secretion levels from MBMSCs may be used as a predictable marker of bone formation capacity in vivo .

show abstract

Bone regeneration capacities of alveolar bone mesenchymal stem cells sheet in rabbit calvarial bone defect

Cited by 32 publications

References 55 publications

Fabrication and Characterization of Collagen/PVA Dual-Layer Membranes for Periodontal Bone Regeneration

Fabrication and Characterization of Collagen/PVA Dual-Layer Membranes for Periodontal Bone Regeneration

Neural crest-like stem cells for tissue regeneration

Efficacy of chitinase-3-like protein 1 as an in vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells

Contact Info

Product

Resources

About