Fabrication and Characterization of Collagen/PVA Dual-Layer Membranes for Periodontal Bone Regeneration

Abstract

Guided tissue regeneration (GTR) is a promising treatment for periodontal tissue defects, which generally uses a membrane to build a mechanical barrier from the gingival epithelium and hold space for the periodontal regeneration especially the tooth-supporting bone. However, existing membranes possess insufficient mechanical properties and limited bioactivity for periodontal bone regenerate. Herein, fish collagen and polyvinyl alcohol (Col/PVA) dual-layer membrane were developed via a combined freezing/thawing and layer coating method. This dual-layer membrane had a clear but contact boundary line between collagen and PVA layers, which were both hydrophilic. The dual membrane had an elongation at break of 193 ± 27% and would undergo an in vitro degradation duration of more than 17 days. Further cell experiments showed that compared with the PVA layer, the collagen layer not only presented good cytocompatibility with rat bone marrow-derived mesenchymal stem cells (BMSCs), but also promoted the osteogenic genes (RUNX2, ALP, OCN, and COL1) and protein (ALP) expression of BMSCs. Hence, the currently developed dual-layer membranes could be used as a stable barrier with a stable degradation rate and selectively favor the bone tissue to repopulate the periodontal defect. The membranes could meet the challenges encountered by GTR for superior defect repair, demonstrating great potential in clinical applications.

Development of Polyvinyl Alcohol/Kaolin Sponges Stimulated by Marjoram as Hemostatic, Antibacterial, and Antioxidant Dressings for Wound Healing Promotion

“…Furthermore, cross-sectional micrographs of the PVA/marjoram and PVA/marjoram/kaolin sponges exposed asymmetric neat structures with prominent three-dimensional interconnected networks as portrayed in Figure 4 a–g. Furthermore, the sponge composites showed porous sponge layers with evident lamellar structures comparable to previously applied PVA sponge composites in wound healing with remarkable performances [ 13 , 26 ]. The sponge with a dense structure, on the other hand, is not favorable for hemostatic wound dressings, which necessitates high conductivity to the wound for absorbing wound surplus exudate and interacting with blood to enhance the hemostatic reaction [ 26 , 27 ].…”

Hierarchical Intrafibrillarly Mineralized Collagen Membrane Promotes Guided Bone Regeneration and Regulates M2 Macrophage Polarization

“…However, among existing barrier materials for GBR, most absorbable membranes lack mechanical properties to maintain the space long enough for bone regeneration, whereas non-absorbable membranes do not degrade and require a second operation for their removal. Thus, both types of membranes are insufficient for clinical application (Zhou et al, 2021).…”

Rationally designed bioactive milk-derived protein scaffolds enhanced new bone formation

“…Second, to examine the influence of CPP and cytokines secreted from cultured RAW 264.7 cells on different scaffolds on the osteogenic differentiation rate, we performed in vitro osteogenic differentiation tests. Only the PVA group was exclused from the in vitro osteogenic differentiation tests because various in vitro and in vivo studies reported the inadequate osteogenic performance of PVA [ [39] , [40] , [41] ]. Before culturing bmMSCs with MDP or MDP-DOPA scaffolds, we collected conditioned media (CM) from RAW 264.7 cells cultured on each scaffold for 1 day ( Fig.…”