Bone regeneration by basic fibroblast growth factor complexed with biodegradable hydrogels

Tabata, Yasuhiko; Yamada, Keisuke; Miyamoto, Susumu; Nagata, Isamu; Kikuchi, Hiroe; Aoyama, Ikuo; Tamura, Makoto; Ikada, Yoshito

doi:10.1016/s0142-9612(98)00233-6

Cited by 202 publications

(142 citation statements)

References 24 publications

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“…bFGF has been reported to promote bone healing (even with a single injection of bFGF) in various bone fracture models of various animals [19,20,27]. The use of a bFGF in combination with a carrier may result in more potent bone regeneration than a single injection [22,26]. For example, gelatin incorporating 30 µg bFGF was shown to produce more regenerated bone than gelatin alone in mandibular bone defects in dogs [18].…”

Section: Discussionmentioning

confidence: 99%

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Changes in Bone Regeneration by Trehalose Coating and Basic Fibroblast Growth Factor after Implantation of Tailor-Made Bone Implants in Dogs

Choi

Lee

Igawa

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. In this study, we aimed to determine the effect of trehalose coating and the optimal dose of basic fibroblast growth factor (bFGF), an osteoinductive protein, loaded onto tailor-made bone implants for implant-induced bone formation in vivo. We fabricated tailor-made α-tricalcium phosphate bone implants (11 mm diameter with 2 parallel cylindrical holes). bFGF 0, 1, 10, 100 or 200 µg/implant was incorporated into implants with and without a trehalose coating, and these were subsequently implanted into dogs to correct temporal bone defects of the same size and shape. Four weeks after implantation, we analyzed the bone implants and surrounding tissues by using microcomputed tomography imaging and histological analyses, as well as gross evaluation. No significant difference in new bone formation was observed between implants with and without a trehalose coating at any of the bFGF doses. Bone implants with 100 and 200 µg bFGF showed significantly more new bone formation at the implant site and within the cylindrical holes of the implants than those without bFGF (P<0.05). However, heterotopic bone formation on the skull near the implant was observed in the group that received 200 µg bFGF. These results suggest that 100 µg bFGF is the optimal dose for this implant in dogs, and that the trehalose coating may not be necessary in vivo, probably due to the presence of blood proteins and electrolytes at the implant site. KEY WORDS: bFGF, bone graft, in vivo, osteoinductive, tailor-made bone implant.

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Section: Discussionmentioning

confidence: 99%

“…A single injection of bFGF increases bone mass [29], but an injection of bFGF alone into bone defects was found to induce significantly less bone formation than bFGF injected in combination with a carrier [26]. Therefore, various carriers were studied to improve the efficacy of bFGF by controlling its release rate [2,10].…”

mentioning

confidence: 99%

Changes in Bone Regeneration by Trehalose Coating and Basic Fibroblast Growth Factor after Implantation of Tailor-Made Bone Implants in Dogs

Choi

Lee

Igawa

et al. 2013

The Journal of Veterinary Medical Science

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“…Therefore it is necessary to develop a drugdelivery system to gradually release bFGF in vivo and extend the biological activity. Recently, studies have shown that biodegradable gelatin hydrogels could incorporate bFGF and release it sustainedly in vivo to induce regeneration and reconstruction of host tissues 8,9 .…”

Section: Introductionmentioning

confidence: 99%

Reconstruction of abdominal wall defects using small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor

et al. 2014

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. Design of the study, critical revision. ABSTRACT PURPOSE:To construct a new biomaterial-small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor, and to evaluate the new biomaterials for the reconstruction of abdominal wall defects. METHODS:Thirty six Sprague-Dawley rats were used in the animal experiments and randomly divided into three groups. The new biomaterial was constructed by combining small intestinal submucosa with gelatin hydrogel for basic fibroblast growth factor release. Abdominal wall defects were created in rats, and repaired using the new biomaterials (group B), compared with small intestinal submucosa (group S) and ULTRAPRO TM mesh (group P). Six rats in each group were sacrificed at three and eight weeks postoperatively to examine the gross effects, inflammatory responses, collagen deposition and neovascularization. RESULTS:After implantation, mild adhesion was caused in groups B and S. Group B promoted more neovascularization than group S at three weeks after implantation, and induced significantly more amount of collagen deposition and better collagen organization than groups S and P at eight weeks after implantation. CONCLUSION:Small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor could promote better regeneration and remodeling of host tissues for the reconstruction of abdominal wall defects.Key words: Abdominal Wall. Biocompatible Materials. Intestinal, Fibroblast Growth Factor 2. Rats. Reconstruction of abdominal wall defects using small intestinal submucosa coated withgelatin hydrogel incorporating basic fibroblast growth factor

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“…During the early bone healing stage, the defect spaces at the interface between the implant and graft material (HA or xenogeneic bone) were filled with blood clots that were soon replaced by fibrous granulation tissue. These fibrous tissue sites gave rise to bone under stimulation by bone morphogenetic protein and other growth factors 18,19) . poly(P) appears to enhance bone regeneration by facilitating this process.…”

Section: Discussionmentioning

confidence: 99%

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

et al. 2014

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Inorganic polyphosphate (poly(P)) is recognized as a therapeutic agent that promotes fibroblast growth factor and enhances osteogenic differentiation, and in vivo, when adsorbed onto interconnected porous calcium hydroxyapatite (IP-CHA) enhances bone regeneration. The present study focused on the effect of poly(P) adsorbed onto IP-CHA granules (Poly(P)/IP-CHA) in guided bone regeneration (GBR). Dental implants were placed into the edentulous mandibular areas of five Beagle-Labrador hybrid dogs with screw expose on the buccal side, and then bone defects were filled Poly(P)/IP-CHA (test) or IP-CHA (control). After 12 weeks, histological evaluation and histomorphometrical analysis were performed. Newly-bone formation around exposed implant screw was clearly detected in the test-group. The ratio for regenerated bone height in the test group versus the control-group was 85.6±20.2 and 62.6±23.8, respectively, with no significant difference, while, that for bone implant contact was significantly higher (67.9±11.8 and 48.8±14.1, respectively). These findings indicate that Poly(P)/IP-CHA enhances bone regeneration in GBR.

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Bone regeneration by basic fibroblast growth factor complexed with biodegradable hydrogels

Cited by 202 publications

References 24 publications

Changes in Bone Regeneration by Trehalose Coating and Basic Fibroblast Growth Factor after Implantation of Tailor-Made Bone Implants in Dogs

Changes in Bone Regeneration by Trehalose Coating and Basic Fibroblast Growth Factor after Implantation of Tailor-Made Bone Implants in Dogs

Reconstruction of abdominal wall defects using small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

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