Bone-protecting effect of Rubus coreanus by dual regulation of osteoblasts and osteoclasts

Abstract

The present data provide the first direct in vivo evidence that RCM has a bone-protecting effect caused by estrogen deficiency, without undesirable side effects on the uterus and other solid organs. The beneficial effect of RCM may be mediated, at least in part, by dual regulation of the enhancement of osteoblast function and induction of osteoclast apoptosis.

Effects of Different Varieties of Maca <i>(Lepidium meyenii)</i> on Bone Structure in Ovariectomized Rats

“…In fact, bone mass is regulated by a dynamic and complex process called remodeling, characterized by a balance between osteoclastic bone resorption and osteoblastic bone formation [33]. Polyphenolic compounds play a bone-protective role by both reducing osteoclastic resorption and increasing osteoblastic activity [34][35][36][37]. This action can be related to the antioxidant capacity of polyphenols [38].…”

Effect of Rubus coreanus extracts on diabetic osteoporosis by simultaneous regulation of osteoblasts and osteoclasts

“…Treatment of the osteoblastic cell line MC3T3-E1 with RCM caused an increase in the expression of intracellular bone ALP. 14,15 Semiquantitative RT-PCR revealed increased OCN expression in the STZ + RCM and OVX + STZ + RCM groups. OCN is considered a specific bone marker of osteoblastic differentiation and activation.…”

“…The prepared RCM extracts were dissolved in saline and orally administered to the STZ + RCM and OVX + STZ + RCM groups at concentrations of 400 mg/kg, 5 days per week for 6 weeks. The dose of RCM was according to that used in the study by Do et al 14 and determined after the pilot study. All abnormal clinical signs and general behavioral changes were observed once a day, and body weights were measured at the day of dosing (day 0) and every week during the experimental period.…”

“…To date, only efficacy studies using an ovariectomized rat model and osteoblastic cell line have been reported. 14,15 Moreover, it has been reported that bisphosphonate or hormone therapies for the treatment of osteoporosis have some adverse effects such as osteonecrosis of the jaw and atrial fibrillation. Therefore, research focusing on natural substances to discover alternative medicine therapies rather than chemicals has increased.…”

Ethyl-3,4-Dihydroxybenzoate with a Dual Function of Induction of Osteogenic Differentiation and Inhibition of Osteoclast Differentiation for Bone Tissue Engineering

“…1), and the extract has recently been shown to have a bone protecting-effect on postmenopausal osteoporosis in ovariectomized rats. 10 EDHB reduces collagen production in keloid fibroblasts 11 and smooth muscle cells, 12 and it inhibits myoblast differentiation. 13 Also, EDHB regulates heme oxygenase-1 mRNA expression through hypoxia-inducible factor-1a-independent mechanisms 14 ; however, the dual function of EDHB of inducing osteoblastic differentiation and inhibiting osteoclast differentiation has not been previously evaluated and warrants further evaluation.…”