Effect of Rubus coreanus extracts on diabetic osteoporosis by simultaneous regulation of osteoblasts and osteoclasts

Choi, Chan-Woong; Lee, HyeRim; Lim, HaeKyoung; Park, So‐Hyun; Lee, Jiwon; Do, Sun-Hee

doi:10.1097/gme.0b013e31824b1cc5

Cited by 23 publications

(16 citation statements)

References 43 publications

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“…CRL-2593, Manassas, USA) were maintained in α-minimum essential medium (α-MEM; Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Gibco) and 1% antibiotics (Gibco). Differentiation induction medium was prepared by adding 5 mM A-glycerol phosphate (Sigma-Aldrich, St. Louis, MO, USA) and 50 ng/mL vitamin C (Sigma-Aldrich) to the culture medium [20], [21].…”

Section: Methodsmentioning

confidence: 99%

Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo

Lee

Kim

et al. 2013

PLoS ONE

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Porous calcium phosphate ceramics are used in orthopedic and craniofacial applications to treat bone loss, or in dental applications to replace missing teeth. The implantation of these materials, however, does not induce stem cell differentiation, so suitable additional materials such as porous calcium phosphate discs are needed to influence physicochemical responses or structural changes. Rabbit adipose-derived stem cells (ADSC) and mouse osteoblastic cells (MC3T3-E1) were evaluated in vitro by the MTT assay, semi-quantitative RT-PCR, and immunoblotting using cells cultured in medium supplemented with extracts from bioceramics, including calcium metaphosphate (CMP), hydroxyapatite (HA) and collagen-grafted HA (HA-col). In vivo evaluation of the bone forming capacity of these bioceramics in rat models using femur defects and intramuscular implants for 12 weeks was performed. Histological analysis showed that newly formed stromal-rich tissues were observed in all the implanted regions and that the implants showed positive immunoreaction against type I collagen and alkaline phosphatase (ALP). The intramuscular implant region, in particular, showed strong positive immunoreactivity for both type I collagen and ALP, which was further confirmed by mRNA expression and immunoblotting results, indicating that each bioceramic material enhanced osteogenesis stimulation. These results support our hypothesis that smart bioceramics can induce osteoconduction and osteoinduction in vivo, although mature bone formation, including lacunae, osteocytes, and mineralization, was not prominent until 12 weeks after implantation.

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Section: Methodsmentioning

confidence: 99%

Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo

Lee

Kim

et al. 2013

PLoS ONE

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“…OC is responsible for osteolysis and dissolution of the bone matrix via bone resorption, leading to lacuna formation and bone reconstruction Zhang et al, 2006). Recently, it was demonstrated that the functions of OB and OC showed correlative dependence (Choi et al, 2012;Yamashita et al, 2012). OB could regulate OC maturation and activation by secreting interleukin and other cytokines (Quan et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

IL-23 promotes osteoclastogenesis in osteoblast-osteoclast co-culture system

Kang¹,

Zhang²

2014

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to determine the effect of IL-23 on the activity and proliferation of osteoclasts (OC) in co-culture with osteoblasts (OB). OB and OC were individually separated from the skull and femoral bone of a SD rat. OB-OC co-culture with IL-23 added was designed as the experimental group, while the OB-OC co-culture without IL-23 was the control group. In the experimental group, five different concentrations of IL-23 were added, and the cells were then cultured for 24, 48 and 72 h. For each concentration at these three time points, cell proliferation, tartrate-resistant acid phosphatase (TRAP) activity and the lacunae in the bone slices were evaluated, compared with control group at the same time points. Compared to the control group, proliferation and TRAP activity of OC were significantly increased at 24, 48 and 72 h with addition of 0.5 to 10 ng/mL IL-23 (P < 0.05). In addition, a dose-and time-dependent correlation between the effect of IL-23 and osteoclastogenesis was noticed though the comparison. Moreover, the area of lacunar resorption in each experimental group was significantly larger than in the control group (P

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“…Diabetic osteoporosis (DOP) is a kind of systemic and metabolic bone disease that is prone to fractures [15,16]. With the increasing prevalence of diabetes, diabetic osteoporosis has become one of the main reasons that decline the life quality of diabetic patients [17]. Liuwei Dihuang Decoction consists of six herbal drugs including Rehmanniae Radix Praeparata, Moutan Cortex, Dioscoreae Rhizoma, Corni Fructus, Alismatis Rhizoma, and Poria [18].…”

Section: Introductionmentioning

confidence: 99%

Preparation and application of polyvinyl alcohol‐decorated cell membrane chromatography for screening anti‐osteoporosis components from Liuwei Dihuang decoction‐containing serum

Wang²,

Xu³

et al. 2020

J of Separation Science

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Cell membrane chromatography is a powerful tool for screening active components from complex matrices. New cell membrane carriers need to be developed to increase the coverage of cell membranes on the surface of stationary phases, thereby improving cell membrane chromatographic retention. In this work, we prepared polyvinyl alcohol-poly dimethyl diallyl ammonium chloride modified silica gel as a cell membrane carrier. Osteoblast cell was used as cell membrane source, which was widely used to evaluate the osteogenic activity of drugs. The new cell membrane chromatographic stationary phase was used to screen anti-osteoporosis components in Liuwei Dihuang decoction-containing serum. The chemical structures of the new modified materials were characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, and elemental analysis characterization.Compared with the common cell membrane column, the cell membrane coverage of this modified material was increased by 30%, and thus provided a stronger retention effect in positive drugs. Nineteen metabolites in rat serum samples were retained on the cell membrane chromatography and identified by ultra-high-performance liquid chromatography/time-of-flight mass spectrometry. Among those, four components (morroniside, catalpol, loganin, and acteoside) were selected for in vitro pharmacodynamics validation. They significantly increased the osteoblast proliferation. The new modified material was successfully applied to screen anti-osteoporosis components from Liuwei Dihuang Decoction-containing serum. K E Y W O R D Sosteoblast cell membrane chromatography, polyvinyl alcohol-decorated, metabolites, rat serum, traditional Chinese medicineArticle Related Abbreviations: CMC, cell membrane chromatography; OM, osteoblast cell membrane; PDAC, poly dimethyl diallyl ammonium chloride; PVA, polyvinyl alcohol; TCM, traditional Chinese medicine.

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Effect of Rubus coreanus extracts on diabetic osteoporosis by simultaneous regulation of osteoblasts and osteoclasts

Cited by 23 publications

References 43 publications

Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo

Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo

IL-23 promotes osteoclastogenesis in osteoblast-osteoclast co-culture system

Preparation and application of polyvinyl alcohol‐decorated cell membrane chromatography for screening anti‐osteoporosis components from Liuwei Dihuang decoction‐containing serum

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