Bone Morphogenetic Proteins

Carreira, Ana Cláudia Oliveira; Zambuzzi, Willian Fernando; Rossi, Mariana Correa; Filho, Renato Astorino; Sogayar, Mari Cleide; Granjeiro, José Mauro

doi:10.1016/bs.vh.2015.06.002

Cited by 68 publications

(19 citation statements)

References 94 publications

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“…Clinical trials have confirmed the efficacy of FDA-approved recombinant human BMP-2 and BMP-7. 13 However, the release of BMPs is not controlled, which leads to local soft-tissue edema, hematoma, heterotopic ossification, and other complications, thus more account of its safety needs to be taken. 14 , 15 Therefore, whether BMP-2 can be released in a controlled manner is the key factor.…”

Section: Introductionmentioning

confidence: 99%

<p>Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model</p>

Wu¹,

Gao²,

Xu³

2020

IJN

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Purpose Developmental dysplasia of the hip (DDH) can increase the pressure between the joints, which causes secondary hip osteoarthritis. The aim of the present study was to fabricate poly(D, L-lactic acid)-poly(ethylene glycol)-poly(D, L-lactic acid) (PELA) electrospun fibrous scaffolds, immobilized with bone morphogenetic protein-2 (BMP-2), to repair the acetabulum defects. Methods The characteristics of PELA electrospun were analyzed using scanning electron microscopy, the release kinetics of BMP-2 in vitro were analyzed using enzyme-linked immunosorbent assays. Human mesenchymal stem cells (hMSCs) were used for in vitro experiments, the biocompatibility of the electrospinning materials was investigated using a cell counting kit-8 (CCK-8) kit, and osteogenic differentiation was tested via alkaline phosphatase (ALP) activity and relative gene expression. Eighteen miniature pig animal models were used in the in vivo experiment. The pigs were sacrificed at 24 weeks after surgery, and the reconstructed acetabulum was evaluated using histological sections. Results Structural analysis revealed that the diameter of the PELA electrospun fiber was 0.8195 ± 0.16 μm. The PELA electrospun fiber materials showed good hydrophilicity and biocompatibility and could continuously release BMP-2 within 27 days: 16.07 ± 0.11 ng of BMP-2 was released from the scaffold. A total of sixteen implants fully filled the defects, and hematoxylin and eosin staining and Goldner’s trichrome staining showed that the simple tendon group (T group) was mostly fibrous tissues, lots of fibroblasts and small amounts of chondrocytes were observed in the polydopamine-coated electrospun fiber group (DP group). The DP plus BMP-2 (DPB) group showed a large number of chondrocytes and partial new bone tissues. Conclusion PELA electrospun fibrous scaffolds are good sustained-release carriers, which can not only induce implant differentiation into cartilage and bone but also are completely degraded without toxicity. Therefore, the material can be used for bone and cartilage regeneration.

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Section: Introductionmentioning

confidence: 99%

<p>Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model</p>

Wu¹,

Gao²,

Xu³

2020

IJN

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“…Alternative techniques based on gene transduction using naked DNA or viral vectors for the expression of BMPs can be option for the development of self-stimulated implants that steadily express growth factors at the site of the lesion, in large and small mammals [29, 35–37]. However, the injection of AdBMP2 and Ad-BMP7 in the site of an osteotomy in 450 kg equines to induce osteogenesis after a 16-week evaluation did not show differences between the control and the transduced groups [38].…”

Section: Discussionmentioning

confidence: 99%

Implant Composed of Demineralized Bone and Mesenchymal Stem Cells Genetically Modified with AdBMP2/AdBMP7 for the Regeneration of Bone Fractures in Ovis aries

Hernandez-Hurtado

Borrego‐Soto

Marino-Martínez

et al. 2016

Stem Cells International

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Adipose-derived mesenchymal stem cells (ADMSCs) are inducible to an osteogenic phenotype by the bone morphogenetic proteins (BMPs). This facilitates the generation of implants for bone tissue regeneration. This study evaluated the in vitro osteogenic differentiation of ADMSCs transduced individually and in combination with adenoviral vectors expressing BMP2 and BMP7. Moreover, the effectiveness of the implant containing ADMSCs transduced with the adenoviral vectors AdBMP2/AdBMP7 and embedded in demineralized bone matrix (DBM) was tested in a model of tibial fracture in sheep. This graft was compared to ewes implanted with untransduced ADMSCs embedded in the same matrix and with injured but untreated animals. In vivo results showed accelerated osteogenesis in the group treated with the AdBMP2/AdBMP7 transduced ADMSC graft, which also showed improved restoration of the normal bone morphology.

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“…As an effective osteoinductive cytokine [ 1 ], bone morphogenetic protein-2 (BMP-2) is commonly applied to promote osteogenesis clinically [ 2 ]. In 2002, the FDA approved the use of the recombinant human bone morphogenetic protein-2 (rhBMP-2) for anterior lumbar interbody fusion [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Zanthoxylum nitidum extract attenuates BMP-2-induced inflammation and hyperpermeability

Luo

et al. 2020

Bioscience Reports

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Bone morphogenetic protein-2 (BMP-2) is commonly applied in spinal surgery to augment spinal fusion. Nevertheless, its pro-inflammatory potential could induce dangerous side effects such as vascular hyper-permeability, posing the need for manners against this condition. This study aims to investigate the protective effect of Zanthoxylum nitidum (ZN) on BMP-2 related hyperpermeability and inflammation on the human umbilical vein endothelial cells (HUVECs). The results revealed that, in a concentration-dependent manner, BMP-2 enhanced the production of pro-inflammatory cytokines, including interleukin (IL)-1α, IL-1β, and tumor necrosis factor-α, which were, however, suppressed by ZN. ZN inhibited BMP-2-induced inflammatory response by suppressing the phosphorylation of NF-κBp65 and IκB, and the abnormal nuclear translocation of p65. Moreover, the inhibited expression intercellular tight junction protein VE-cadherin and Occludin caused by BMP-2 was blocked by ZN. The hyper-permeability of HUVECs induced by BMP-2, as expressed as the higher fluorescent intensity of dextran, was also reversed by ZN. Overall, these findings demonstrated that ZN antagonized BMP2-induced inflammation and hyperpermeability. It could be a therapeutic candidate for the treatment of BMP-2-induced side effects during spinal fusion.

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Bone Morphogenetic Proteins

Cited by 68 publications

References 94 publications

<p>Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model</p>

<p>Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model</p>

Implant Composed of Demineralized Bone and Mesenchymal Stem Cells Genetically Modified with AdBMP2/AdBMP7 for the Regeneration of Bone Fractures in Ovis aries

Zanthoxylum nitidum extract attenuates BMP-2-induced inflammation and hyperpermeability

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