Bone marrow stromal cells induce myeloid and lymphoid development of the sorted hematopoietic stem cells in vitro

Okuyama, Rin; Koguma, Masahito; Yanai, Nobuaki; Obinata, Masuo

doi:10.1182/blood.v86.7.2590.bloodjournal8672590

Cited by 4 publications

(5 citation statements)

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“…Similar to our findings in canine long-term marrow culture, Okuyama et al have described a biphasic development of myeloid (first wave) and lymphoid (second wave) cells in an in Vitro model in which mouse purified stem cells were cultured over a stromal cell line (35). Appearance of myeloid and lymphoid cells has been also reported in the SCID-hu system (36).…”

Section: Discussionsupporting

confidence: 89%

“…It is likely Tissue Antigens 1998: 51: 141-155 that in myelopoiesis, the hematopoietic cells must adhere to the stromal cells through VLA-4-VCAM-1 interaction, but their continuous interaction is not required for further proliferation and differentiation of the hematopoietic cells. However, their continuous interaction may be required for lymphopoiesis (35).…”

Section: Discussionmentioning

confidence: 99%

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The expression and differentiation pattern of cell antigens and adhesion molecules on the nonadherent cell population in canine long‐term marrow culture: a biphasic development of myeloid and lymphoid cells

et al. 1998

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During maturation of normal hematopoietic progenitors, there appears to be differentiation‐dependent expression of adhesion receptors, which may contribute to the homing and lodging of stem progenitor cells into the marrow and for the eventual release of mature effector cells from the marrow cavity. Using a model of long‐term marrow culture, we studied the expression pattern of different lineage cell antigens and cell adhesion molecules on the nonadherent cells in canine long‐term marrow culture. CD4+ cells became a major proportion of both the small and large cell subsets by day 8 of culture. Small CD4+ cells, the majority of which are negative for other T‐cell antigens during the first 2 weeks of culture, express low levels of CD4 (CD41°) and coexpress granulocytic and/or monocytic markers. These CD41° cells have progenitor potential as measured by the long‐term culture‐initiating cell assay and differentiate into myeloid (first wave) and lymphoid (second wave) cells. The T cells, which appear in 2‐week‐old long‐term marrow culture, respond to Con A but not to allo‐antigen. At the same time, most of the large cells are CD14+, CD11b+, DLA‐DR+ and CD4lo+, while granulocytes are not observed. This phenotypic pattern closely resembles that found on myelomonocytic cells developing from fetal thymic and fetal liver CD34+ precursors in a model of human fetal thymic organ culture. The cell adhesion molecules ‐ CD44, CD18, l‐selectin, CDlla‐c as well as VLAα4, dramatically increase from the first week of long‐term marrow culture, while ICAM‐1 and a new β2 cell adhesion molecule, αdβ2, increased slightly from the third week on. In the large (“monocytic”) cell population, αdβ2 was exclusively expressed by CD4+ cells. The differentiation pattern of T‐cell antigens and adhesion molecules seen in the canine long‐term marrow culture appear to mimic those required for developmental interactions between leukocytes and endothelial cells; that is, an early expression of L‐selectin and CD44, followed by the integrins, and later on by ICAM‐1 and αdβ2. Our data support the view that in a model of canine long‐term marrow culture hematopoietic precursors retain their intrinsic developmental potential.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

The expression and differentiation pattern of cell antigens and adhesion molecules on the nonadherent cell population in canine long‐term marrow culture: a biphasic development of myeloid and lymphoid cells

et al. 1998

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“…Yolk sac cells were inoculated into 0.8% methylcellulose and cultured in the presence of erythropoietin (2 U/ml) and stem cell factor (SCF; 50 ng/ml) (Okuyama et al, 1995), and the numbers of colonies (colonyforming unit-erythroid, CFU-E) formed were counted.…”

Section: Colony Assaymentioning

confidence: 99%

Heme deficiency in erythroid lineage causes differentiation arrest and cytoplasmic iron overload

Nakajima

Takahashi

Harigae

et al. 1999

EMBO J

101

100

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Erythroid 5-aminolevulinate synthase (ALAS-E) catalyzes the first step of heme biosynthesis in erythroid cells. Mutation of human ALAS-E causes the disorder X-linked sideroblastic anemia. To examine the roles of heme during hematopoiesis, we disrupted the mouse ALAS-E gene. ALAS-E-null embryos showed no hemoglobinized cells and died by embryonic day 11.5, indicating that ALAS-E is the principal isozyme contributing to erythroid heme biosynthesis. In the ALAS-E-null mutant embryos, erythroid differentiation was arrested, and an abnormal hematopoietic cell fraction emerged that accumulated a large amount of iron diffusely in the cytoplasm. In contrast, we found typical ring sideroblasts that accumulated iron mostly in mitochondria in adult mice chimeric for ALAS-E-null mutant cells, indicating that the mode of iron accumulation caused by the lack of ALAS-E is different in primitive and definitive erythroid cells. These results demonstrate that ALAS-E, and hence heme supply, is necessary for differentiation and iron metabolism of erythroid cells.

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“…Examination of the supporting ability of two typical stromal cell lines showed that TBR59 can induce cobblestone formations committed to myelopoiesis and lymphopoiesis of the sorted stem cells (Okuyama et al 1995a). Another cell line, TBR31-1 was shown to specifically induce B-lymphopoiesis from the same sorted stem cell population.…”

Section: Bone Marrow Stromal Cell Lines That Support Growth and Diffementioning

confidence: 99%

Conditionally immortalized cell lines with differentiated functions established from temperature‐sensitive T‐antigen transgenic mice

Obinata

1997

Genes to Cells

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A variety of cell lines with differentiated functions are required to study tissue functions at cellular and molecular levels. Using transgenic mice harbouring ubiquitously expressing the temperaturesensitive T-antigen (ts T-antigen) gene of simian virus 40 (SV40), many cell lines were generated. The properties of these established lines suggested that their growth was dependent on T-antigen, and that they retained some of the differentiated functions of each particular tissue. A possible use of these cell lines for tissue functions in vitro is discussed.

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Bone marrow stromal cells induce myeloid and lymphoid development of the sorted hematopoietic stem cells in vitro

Cited by 4 publications

References 0 publications

The expression and differentiation pattern of cell antigens and adhesion molecules on the nonadherent cell population in canine long‐term marrow culture: a biphasic development of myeloid and lymphoid cells

The expression and differentiation pattern of cell antigens and adhesion molecules on the nonadherent cell population in canine long‐term marrow culture: a biphasic development of myeloid and lymphoid cells

Heme deficiency in erythroid lineage causes differentiation arrest and cytoplasmic iron overload

Conditionally immortalized cell lines with differentiated functions established from temperature‐sensitive T‐antigen transgenic mice

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