Bone Marrow Stimulation Technique Augmented by an Ultrapurified Alginate Gel Enhances Cartilage Repair in a Canine Model

Baba, Rikiya; Onodera, Tomohiro; Matsuoka, Morito; Hontani, Kazutoshi; Joutoku, Zenta; Matsubara, Shin; Homan, Kentaro; Iwasaki, Norimasa

doi:10.1177/0363546518770436

Cited by 15 publications

(27 citation statements)

References 52 publications

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“…Although it is difficult to compare the results of our study with this one because of different factors, the stiffness of regenerated tissue in the UPAL gel group was almost identical to that in our 2 previous studies. 6,7 Nonetheless, we and the authors of the other study agree that longer duration may improve the stiffness of regenerated tissue.…”

Section: Discussionsupporting

confidence: 57%

Effects of Ultra-Purified Alginate Gel Implantation on Meniscal Defects in Rabbits

et al. 2018

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Background: Many tissue-engineered methods for meniscal repair have been studied, but their utility remains unclear. Hypothesis: Implantation of low-endotoxin, ultra-purified alginate (UPAL) gel without cells could induce fibrocartilage regeneration on meniscal defects in rabbits. Study Design: Controlled laboratory study. Methods: Forty-two mature Japanese White rabbits were divided into 2 groups of 21 animals each. In each animal, a cylindrical defect measuring 2 mm in diameter was created with a biopsy punch on the anterior horn of the medial meniscus. In the control group, no treatment was applied on the left medial meniscal defect. In the UPAL gel group, the right medial meniscal defect was injected with the UPAL gel and gelated by a CaCl2 solution. Samples were evaluated at 3, 6, and 12 weeks postoperatively. For biomechanical evaluation, 6 additional samples from intact animals were used for comparison. Results: The macroscopic score was significantly greater in the UPAL gel group than in the control group at 3 weeks (mean ± SE: 5.6 ± 0.82 vs 3.4 ± 0.83, P = .010), 6 weeks (5.9 ± 0.72 vs 2.5 ± 0.75, P = .026), and 12 weeks (5.2 ± 1.21 vs 1.0 ± 0.63, P = .020). The histological score was significantly greater in the UPAL group than in the control group at 3 weeks (2.1 ± 0.31 vs 1.2 ± 0.25, P = .029) and 12 weeks (2.2 ± 0.55 vs 0.3 ± 0.21, P = .016). The mean stiffness of the reparative tissue in the UPAL gel group was significantly greater than that in the control group at 6 weeks (24.325 ± 3.920 N/mm vs 8.723 ± 1.190 N/mm, P = .006) and at 12 weeks (27.804 ± 6.169 N/mm vs not applicable [because of rupture]). Conclusion: The UPAL gel enhanced the spontaneous repair of fibrocartilage tissues in a cylindrical meniscal defect in rabbits. Clinical Relevance: These results imply that the acellular UPAL gel may improve the repair of traumatic meniscal injuries.

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Section: Discussionsupporting

confidence: 57%

Effects of Ultra-Purified Alginate Gel Implantation on Meniscal Defects in Rabbits

et al. 2018

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“…As far as results were concerned, Quiang et al [43] and Baba et al [45] found similar trends between histological and biomechanical analyses; both works measured the cartilage stiffness by means of the indentation test, whereas in the work of Quiang, histomorphometry was conducted by measuring the 3D bone volume by microCT, and in the work of Baba, semiquantitative Niederauer and Changoor scores were adopted.…”

Section: Resultsmentioning

confidence: 93%

Current Trends in the Evaluation of Osteochondral Lesion Treatments: Histology, Histomorphometry, and Biomechanics in Preclinical Models

Maglio

Brogini

Pagani

et al. 2019

BioMed Research International

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Osteochondral lesions (OCs) are typically of traumatic origins but are also caused by degenerative conditions, in primis osteoarthritis (OA). On the other side, OC lesions themselves, getting worse over time, can lead to OA, indicating that chondral and OC defects represent a risk factor for the onset of the pathology. Many animal models have been set up for years for the study of OC regeneration, being successfully employed to test different treatment strategies, from biomaterials and cells to physical and biological adjuvant therapies. These studies rely on a plethora of post-explant investigations ranging from histological and histomorphometric analyses to biomechanical ones. The present review aims to analyze the methods employed for the evaluation of OC treatments in each animal model by screening literature data within the last 10 years. According to the selected research criteria performed in two databases, 60 works were included. Data revealed that lapine (50% of studies) and ovine (23% of studies) models are predominant, and knee joints are the most used anatomical locations for creating OC defects. Analyses are mostly conducted on paraffin-embedded samples in order to perform histological/histomorphometric analyses by applying semiquantitative scoring systems and on fresh samples in order to perform biomechanical investigations by indentation tests on articular cartilage. Instead, a great heterogeneity is pointed out in terms of OC defect dimensions and animal's age. The choice of experimental times is generally adequate for the animal models adopted, although few studies adopt very long experimental times. Improvements in data reporting and in standardization of protocols would be desirable for a better comparison of results and for ethical reasons related to appropriate and successful animal experimentation.

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“…[10][11][12] Low durability of the repaired cartilage is attributed to the new cartilage being fibrocartilage-like, which has inferior mechanical properties to articular hyaline cartilage. 13,14 Gels, such as alginate, have also been used have been used in modified AMIC trials in animal studies, 15 however, hydro-gels provide limited initial support due to inferior mechanical properties and further modifications, e.g. incorporation of matrix derived molecules such as collagen Type I or II, are often required for endogenous stem cell recruitment.…”

Section: Introductionmentioning

confidence: 99%

Scaffold channel size influences stem cell differentiation pathway in 3-D printed silica hybrid scaffolds for cartilage regeneration

Tallia

et al. 2020

Biomater. Sci.

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Bone Marrow Stimulation Technique Augmented by an Ultrapurified Alginate Gel Enhances Cartilage Repair in a Canine Model

Abstract: The data support the clinical reality of 1-step minimally invasive cartilage-reparative medicine with UPAL gel without harvesting donor cells.

Cited by 15 publications

References 52 publications

Effects of Ultra-Purified Alginate Gel Implantation on Meniscal Defects in Rabbits

Effects of Ultra-Purified Alginate Gel Implantation on Meniscal Defects in Rabbits

Current Trends in the Evaluation of Osteochondral Lesion Treatments: Histology, Histomorphometry, and Biomechanics in Preclinical Models

Scaffold channel size influences stem cell differentiation pathway in 3-D printed silica hybrid scaffolds for cartilage regeneration

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