Bone marrow stem cells-derived extracellular matrix is a promising material

Wang, Xiaoyan; Chen, Guanghua; Huang, Chao; Tu, Hualei; Zou, Jilong; Yan, Jinglong

doi:10.18632/oncotarget.21683

Cited by 9 publications

(5 citation statements)

References 37 publications

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“…Accordingly, many studies have evidenced the capacity of specific ECMs, from tissue or cells in culture, to direct stem cell fate toward particular cell lineages. [ 25,33,63–66 ] In this line, interesting results have also been reported by using stepwise‐mimicking matrices derived from stem cells, as it presents a composition that reproduce better the environment and contain adequate factors necessaries for tissue development. [ 22,33 ] Here, cell differentiation could also explain the minimal contraction observed in mdECM hydrogels, slightly more noticeable in the higher concentration (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Development of a Biomimetic Hydrogel Based on Predifferentiated Mesenchymal Stem‐Cell‐Derived ECM for Cartilage Tissue Engineering

Antich

Jiménez

Vicente

et al. 2021

Adv Healthcare Materials

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The use of decellularized extracellular matrix (dECM) as a biomaterial has been an important step forward for the development of functional tissue constructs. In addition to tissues and organs, cell cultures are gaining a lot of attention as an alternative source of dECM. In this work, a novel biomimetic hydrogel is developed based on dECM obtained from mesenchymal stem cells (mdECM) for cartilage tissue engineering. To this end, cells are seeded under specific culture conditions to generate an early chondrogenic extracellular matrix (ECM) providing cues and elements necessary for cartilage development. The composition is determined by quantitative, histological, and mass spectrometry techniques. Moreover, the decellularization process is evaluated by measuring the DNA content and compositional analyses, and the hydrogel is formulated at different concentrations (3% and 6% w/v). Results show that mdECM derived hydrogels possess excellent biocompatibility and suitable physicochemical and mechanical properties for their injectability. Furthermore, it is evidenced that this hydrogel is able to induce chondrogenesis of mesenchymal stem cells (MSCs) without supplemental factors and, furthermore, to form hyaline cartilage-like tissue after in vivo implantation. These findings demonstrate for the first time the potential of this hydrogel based on mdECM for applications in cartilage repair and regeneration.

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Section: Discussionmentioning

confidence: 99%

Development of a Biomimetic Hydrogel Based on Predifferentiated Mesenchymal Stem‐Cell‐Derived ECM for Cartilage Tissue Engineering

Antich

Jiménez

Vicente

et al. 2021

Adv Healthcare Materials

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“…The extraction method for ECM derived from BMSCs is the same as in a previous study. 11 P3 BMSCs were subjected to continued culture under conditions containing ascorbic acid (50 μg/mL) and ascorbate-2-phosphate (150 μg/mL) in a sterile cell culture bottle. After the cells achieved confluence, they were not passaged for 3–4 weeks.…”

Section: Methodsmentioning

confidence: 99%

“…[8][9][10] Stem cells first secrete ECM, which accumulates and matures to form a three-dimensional network structure with abundant organic components, and these components allow the ECM to promote multidirectional differentiation and be applied in tissue regeneration. 11 However, the mechanical properties of BMSC-ECM are poor and the scaffolds produced are not strong enough; therefore, they can only serve as fillers and cannot provide effective support when applied in vivo, thereby limiting their application in bone regeneration. 1 It is necessary for the scaffold to provide 3D cell-growth microenvironments and appropriate synergistic cell-guidance cues to mimic native tissues.…”

Section: Introductionmentioning

confidence: 99%

<p>3D-HA Scaffold Functionalized by Extracellular Matrix of Stem Cells Promotes Bone Repair</p>

Chi¹,

Chen²,

He³

et al. 2020

IJN

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Background and Purpose The extracellular matrix (ECM) derived from bone marrow mesenchymal stem cells (BMSCs) has been used in regenerative medicine because of its good biological activity; however, its poor mechanical properties limit its application in bone regeneration. The purpose of this study is to construct a three dimensional-printed hydroxyapatite (3D-HA)/BMSC-ECM composite scaffold that not only has biological activity but also sufficient mechanical strength and reasonably distributed spatial structure. Methods A BMSC-ECM was first extracted and formed into micron-sized particles, and then the ECM particles were modified onto the surface of 3D-HA scaffolds using an innovative linking method to generate composite 3D-HA/BMSC-ECM scaffolds. The 3D-HA scaffolds were used as the control group. The basic properties, biocompatibility and osteogenesis ability of both scaffolds were tested in vitro. Finally, a critical skull defect rat model was created and the osteogenesis effect of the scaffolds was evaluated in vivo. Results The compressive modulus of the composite scaffolds reached 9.45±0.32 MPa, which was similar to that of the 3D-HA scaffolds ( p >0.05). The pore size of the two scaffolds was 305±47 um and 315±34 um ( p >0.05), respectively. A CCK-8 assay indicated that the scaffolds did not have cytotoxicity. The composite scaffolds had good cell adhesion ability, with a cell adhesion rate of up to 76.00±6.17% after culturing for 7 hours, while that of the 3D-HA scaffolds was 51.85±4.77% ( p <0.01). In addition, the composite scaffold displayed higher alkaline phosphatase (ALP) activity, osteogenesis-related mRNA expression, and calcium nodule formation, thus confirming that the composite scaffolds had good osteogenic activity. The composite scaffolds exhibited good bone repair in vivo and were superior to the 3D-HA scaffolds. Conclusion We conclude that BMSC-ECM is a good osteogenic material and that the composite scaffolds have good osteogenic ability, which provides a new method and concept for the repair of bone defects.

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“…Physically mixing mECM with other materials is another method to generate hybrid scaffolds. Homogenized mECM was suspended in chitosan and gelatin solution in proper proportions and then crosslinked to form a composite scaffold, which promoted MSCs attachment, proliferation, and osteogenic differentiation in vitro [122].…”

Section: Physically Mixing Premade Mecm With Other Scaffoldsmentioning

confidence: 99%

Mesenchymal stem cell-derived extracellular matrix (mECM): a bioactive and versatile scaffold for musculoskeletal tissue engineering

Zhang¹,

Liu²,

Clark³

et al. 2020

Biomed. Mater.

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Mesenchymal stem cell-derived extracellular matrix (mECM) has received increased attention in the fields of tissue engineering and scaffold-assisted regeneration. mECM exhibits many unique characteristics, such as robust bioactivity, biocompatibility, ease of use, and the potential for autologous tissue engineering. As the use of mECM has increased in musculoskeletal tissue engineering, it should be noted that mECM generated from current methods has inherited insufficiencies, such as low mechanical properties and lack of internal architecture. In this review, we first summarize the development and use of mECM as a scaffold for musculoskeletal tissue regeneration and highlight our current progress on moving this technology toward clinical application. Then we review recent methods to improve the properties of mECM that will overcome current weaknesses. Lastly, we propose future studies that will pave the road for mECM application in regenerating tissues in humans.

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Bone marrow stem cells-derived extracellular matrix is a promising material

Cited by 9 publications

References 37 publications

Development of a Biomimetic Hydrogel Based on Predifferentiated Mesenchymal Stem‐Cell‐Derived ECM for Cartilage Tissue Engineering

Development of a Biomimetic Hydrogel Based on Predifferentiated Mesenchymal Stem‐Cell‐Derived ECM for Cartilage Tissue Engineering

<p>3D-HA Scaffold Functionalized by Extracellular Matrix of Stem Cells Promotes Bone Repair</p>

Mesenchymal stem cell-derived extracellular matrix (mECM): a bioactive and versatile scaffold for musculoskeletal tissue engineering

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